Research Papers/Topics in Biochemistry

Analysis Of Low Molecular Weight Compounds Produced By Indigenous Wood Decay Fungi

ABSTRACTOver the years, natural products have played a major role in the search for novel drugsor drug candidates. Secondary metabolites from nature especially those of fungal originexhibit unique biological activities and research continue to meet the keen interestswhich have potential pharmaceutical value. Fungi constitute an important source ofsecondary metabolites such as penicillin. The present study analyzed the biologicalactivities of a variety of compound mixtures from wood decay fung...

Investigations Into The Mechanisms Of Anti-Mycobacterial Drug Resistance Using Antipsychotic Compounds

ABSTRACTThe continual emergence of drug resistant strains of Mycobacterium tuberculosis has caused global public health concerns. This project establishes basis for deciphering diverse resistance mechanisms in mycobacteria which would lead to the development of novel therapeutic options. Unique classes of antipsychotic compounds have been found to possess antifungal and anti-mycobacterial activities. The study sought to use antipsychotic compounds and a panel of phenotype modifying compounds ...

Genetic And Biochemical Characterization Of Pearl Millet Mutant Lines Using Simple Sequence Repeat Markers In Namibia

ABSTRACT Pearl millet is a staple food and fodder crop of marginal areas of sub-Saharan Africa, South Asia and the Americas. Although pearl millet is stable food for 70 % of Namibian population, its productivity is very low (low yield, susceptible to diseases/insect and prolonged reproductive cycle) and has a limited genetic diversity. For the purpose of widening gene diversity, developing high yielding and drought resistant pearl millet, the Kangara and Okashana 2 were mutated using Co...

Proteolytic Activity In Adult Haemonchus Contortos

ABSTRACT , Proteolytic activity of theJQ.qOOxgsupernate of homegenates ef adult Haemonchus contortus has been studied with respect to substrates susceptible to its proteolytic activity, and the effects of factors such as temperature, pH, substrate concentration, extract concentration, dibasic metal ions, detergents, heat and some specific inhibitors of proteolytic activity. The supernate had two pH optima, 5.6 with haemoglobii as substrate, and 7.8 using L-alanyl - p-nitroanilide. It...

Examination of Human Skin Surfaces For The Detection of Mycobacterium Ulcerans

ABSTRACT Mycobacterium ulcerans, causes Buruli ulcer (BU), a necrotizing skin disease endemic in 33 countries globally. Its environmental reservoir and mode of transmission are unknown. Portaels hypothesized that prior skin contamination followed by penetration through existing skin abrasion or trauma may be a possible route through which M. ulcerans is transmitted to humans. Comparative epidemiological and case control studies have outlined some risk activities associated with BU that...

The Effect of Croton Membranaceus on DIHYDROTESTOSTERONE Levels in Blood And its Synthesis by Prostatic 5a-Redutase in The Rat

ABSTRACT Alcoholic extract of Croton membranaceus Mull. Arg is used at the Centre for Scientific Research into Plant Medicine at Mampong Akwapim to treat retention of urine due to benign prostatic hyperplasia (BPH). The effect of the extract on rat steroid 5areductase activity in invo was investigated using a radioimmunoassay technique to quantitate dihydrotestosterone (DHT) levels in blood. The effects of the extract and two unidentified alkaloidal isolates of the plant, provisionally...

Anti-Inflammatory Medicinal Plants As Anti-Oxidants And Inhibitors Of Proinflammatory Eicosanoid Biosynthesis

ABSTRACT Arachidonic acid (AA) metabolism that leads to the production of both anti- and pro-inflammatory eicosanoids is a standard assay used to investigate the basis for the therapeutic action of anti-inflammatory medicinal plants. Earlier investigations have established the efficacy of some herbal preparations in terms of their ability to increase the amounts of anti-inflammatory eicosanoids. Desm.od.ium adscendens and Parkitina sp. ('Tina A' ) increased PGE2 and PGI2 synthesis an...

Association Between Hepcidin, Insulin Resistance And CRP in Type 2 Diabetes Patients at The Korle-Bu Teaching Hospital

ABSTRACT Background: Diabetes Mellitus (DM) is a significant cause of morbidity and mortality and remains a global health challenge. Type 2 diabetes (T2DM) is a leading root of cardiovascular diseases worldwide. Growing evidence indicates a strong association between T2DM and high level of hepcidin; a major regulator of iron homeostasis. Iron overload is an important risk factor that plays a major role in pathogenesis of diabetes and its complications. Insulin resistance and elevated c-...

Maternal Serum Human Chorionic Gonadotropin And Magnesium As Biochemical Markers In Predicting Preeclampsia In Pregnant Ghanaian Women.

Preeclampsia is a multisystem disease of pregnancy of unknown cause. It is a maternal syndrome, which is characterized by increased blood pressure, edema, proteinuria or significant amount of protein in the urine of pregnant women and abnormal clotting, liver and renal functions all of which may be due to the release of placental toxic factors into the mother’s circulation. The only effective therapy to this complication is to facilitate the culteinment of the pregn...

Biochemical Toxicology Of Desmodium Adscendens

Aqueous crude extract of Desmodium adscendens, as dispensed at the Centre for Scientific Research into Plant Medicine (CSRPM), was freeze-dried into a powder and used in this study to evaluate its toxicological effects in rats. For acute toxicity studies, high doses of the freeze-dried extract, were administered orally as single doses to rats, and the behaviours of the animals and organ toxicity were noted. Low, medium and high doses of the plant extract, representing...

Characterizing Pathogenic Risk Factors Associated With Breast Cancer In Ghanaian Women

Cancer is a major health burden worldwide as a result of its high frequency of mortality and morbidity. The most frequent cancer globally among women is breast cancer with a high death rate of about 458,000 deaths per year. Breast cancer is a multi-factorial disease which acts in sequence or simultaneously to initiate and/or promote tumour growth (Ritchie et al., 2001). The origin of breast cancer is poorly understood but it has been associated with are risk factors such a...

Studies On The Distribution Of The Allotypic Variants Of The Igg Receptors (Fcyriia And Fcyrihb) And Their Association With Severe Clinical Malaria Among Ghanaian Children

ABSTRACTThe immunoglobulin G (IgG) receptors FcyRIIa and FcyRIIIb vary among differentethnic groups, and more importantly, are also known to be associated with eithersusceptibility to or protection from certain diseases. These variations are manifest asdifferences in the antigen-binding capabilities of the receptors. The genes encodingfor these receptors are polymorphic and these also vary among populations. Thesevariations can be investigated using molecular methods such as PCR. The aim of t...

Molecular Characterization of Rotaviruses From A SemiUrban Communication in The Central Region of Ghana

ABSTRACT Acute gastroenteritis (AGE) is an inflammation of the intestinal mucosa and is characterised by the onset of diarrhoea with or without vomiting, fever, and abdominal pain. Rotaviruses are the leading cause of AGE in infants and young children resulting in severe dehydration, which can eventually lead to hospitalisation and death. In Ghana, diarrhoea is implicated in the death of about 11% of all children under the age of 5 years, with rotavirus being the most responsible causat...

Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Bruei

ABSTRACT African trypanosomiasis still remains a lethal disease to both human and livestock. The disease persists due to limited drug availability, toxicity and emergence of drug resistance, hence the need to provide alternative forms of therapy. Studies have shown that the iron chelator deferoxamine exhibited anti-trypanosomal effects by inhibiting cell growth and interfering with the activity of some iron dependent enzymes. In this study, the in vitro effects of different phenolic acid...

CYP2C9, VKORC1 AND CYP4F2 Variant Frequencies in Patients on Either Low or High Stable Warfarin Maintenance Therapy in The Ghanaian Population

ABSTRACT Warfarin is the most commonly prescribed oral anticoagulant drug for reducing thromboembolic events that often give rise to stroke, deep vein thrombosis, pulmonary embolism, or serious coronary malfunctions. Warfarin has a narrow therapeutic/toxic ratio and genetic factors have been associated with inter-individual variability in warfarin dose / response in different ethnic populations. The initiation of this drug has been associated with one of the highest adverse event rates ...


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