Microbiology Research Papers/Topics

MICROBIAL CONTAMINATIONS OF MEAT AND ASSOCIATED RISK FACTORS IN BEKOJI TOWN, ARSI ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: The consumption of raw meat has associated with cultural practices and widespread raw beef consumption habit that can be a potential source for food borne illnesses in Ethiopia. Therefore, the present study was intended to assess microbiological contamination and associated risk factors in Bekoji town. A cross sectional study was employed whereby a simple random sampling of Butcher shops in Bekoji Town. The cattle (muscle, liver, and intestine) meat samples were collected aseptical...

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC FUNGI FROM FENNEL (Foeniculum vulgare Mill.) LEAF AND SEED

Abstract: Antioxidant and antimicrobial properties are the biological activity of natural compoundsisolated from medicinal plants that are rich source of novel drugs. Endophytic fungi are a potential source of novel bioactive compounds. The present study investigates antimicrobial and antioxidant properties of endophytic fungi from fennel (Insilali) seeds and leaves. The antioxidant activity was conducted based on determination of phenolic content, 1, 1-diphenyl-2-picrylhydrazyl and hydrogen...

ANALYSIS OF CHEMICAL COMPOSITION OF CACTUS PEAR (OPUNTIA FICUS-INDICA L.) CLADODE EXTRACT AND ITS ROLES IN THE PREPARATION OF FUNGAL CULTURE MEDIA

Abstract: The present study was aimed at analyzing the chemical composition of the crude extract of cactus pear (Opuntia ficus-indica L.) cladode and examine its suitability for fungal culture media. For this purpose cactus pear cladode samples were collected from Haramaya University campus Model School area. The chemical composition analysis was done according to standard procedures. The growth of five fungal species (R. stoloinfer ATCC 1052, P. digitatum ATCC 2560, A. niger ATCC 1232, T. a...

ISOLATION OF MICROORGANISMS (FUNGI) FROM KOCHO AND BULLA FOR ANTIOXIDANT ACTIVITIES

Abstract: Enset (Ensete ventricosum (Welw.) Cheesman) belongs to the family Musaceae, and is one of the oldest cultivated herbaceous and monocarpic banana-like food security crops in Ethiopia. Microbes which colonize living internal tissues of plants without causing any harm to their host can be used for testing their antioxidant potential under controlled conditions. These microbes protect their hosts from adverse conditions by secreting bioactive secondary metabolites. Therefor, the presen...

PHYSIOCHEMICAL AND MICROBIAL QUALITY OF DRINKING WATER, A CASE OF NEGELE ARSI WEREDA, WEST ARSI ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: Water is natural resources and is essentially to sustain life. Poor drinking water quality has been linked to several diseases of man particularly in developing countries, where availability of potable water is a big challenge. Thus, this study was conducted to assess the biological and physico-chemical quality of drinking water at NegaleArsiWoreda.A total of 72 water samples were collected from deep well water. The analysis demonstrated that all the water samples in the study area...

SCREENING AND CHARACTERIZATION KERATINASE ENZYME FROM BACTERIA IN KERATINOUS WAST

Abstract: Microbial keratinases are keratinolytic enzymes widely used in many industrial processes and management of wastes. This study was conducted with the aim of isolation of efficient keratinase producing bacteria form the soils of chicken feather-dumping sites and human hair-dumping places, determining optimum keratinase production conditions and partially characterizing the stability and activity of keratinase with regards to some physicochemical parameters. Plating of soil sample sus...

PRODUCTION OF CELLULASE USING FUNGAL ISOLATES FROM KOCHO (Ensete ventricosum (Welw.) Cheesman)

Abstract: Cellulases are the type of enzymes which catalyze the hydrolysis of cellulose and related oligosaccharide derivatives, is considered a potential tool for industrial saccharification of cellulosic biomass. The production of bio-based products and bioenergy from less costly renewable lignocellulosic materials would bring benefits to the local economy, environment, and national energy security. The processed enset (kocho and bulla) are rich in carbohydrates, and fiber and some mineral...

ANTIOXIDANT AND ANTIMICROBIAL POTENTIALS OF ENDOPHYTIC FUNGIFROM(Lippia adoensis Hoechst.Var. Koseret)LEAF AND SEED

Abstract: Endophytic fungi represent a wide diversity of microbial adaptations that have evolved in special and unusual environments, making them a great source of study and research for new drugs for medical, industrial, and agriculture uses.The present study was undertaken to investigate antioxidant and antimicrobial potential of endophytic fungalextracts ofkoseret leaf and seeds.The leaf and seed samples collected from the home gardens were used for endophyte fungal extraction.Endophytes ...

Surveillance of antibiotic resistance determinants in Gaborone and Palapye wastewater treatment facilities

Abstract: Antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB) have been globally considered emerging environmental contaminants that pose a serious concern to human health and the ecosystem. The ubiquitous nature of ARB and ARGs among pathogens from wastewater effluents, indicate that wastewater treatment facilities are major reservoirs for antibiotic resistance determinants, thus contributing to the widespread dissemination of antibiotic resistance. The primary object...

Characterization of extended-spectrum-β lactamases (esbls) and other resistant genes encoding bacteria from a rural community settlement

Abstract: Antimicrobial resistance (AMR) is regarded as a global health threat, characterized by rising resistant bacteria and rapid development of resistance genes to existing antibiotics used in clinical and veterinary settings. AMR has negative impacts and affects human, animal, and the environment. In Botswana, Africa, AMR is not well understood, particularly in rural settings with poor sanitation. The misuse and overuse of commonly used antibiotics like ß-lactam antibiotics, combi...

A novel moderately thermophilic type Ib methanotroph isolated from an alkaline thermal spring in the Ethiopian Rift Valley

Abstract: Aerobic moderately thermophilic and thermophilic methane-oxidizing bacteria make a substantial contribution in the control of global warming through biological reduction of methane emissions and have a unique capability of utilizing methane as their sole carbon and energy source. Here, we report a novel moderately thermophilic Methylococcus-like Type Ib methanotroph recovered from an alkaline thermal spring (55.4 °C and pH 8.82) in the Ethiopian Rift Valley. The isolate, designate...

Prediction of genes that function in methanogenesis and CO2 pathways in extremophiles

Abstract: Gaet’ale (GAL) and Mud’ara (MUP) are two hypersaline ponds located in the Danakil Depression recharged by underground water from the surrounding highlands. These two ponds have different pH, salinity, and show variation in the concentration of many ionic components. Metagenomic analysis concludes that GAL is dominated by bacteria as in the case of the other hypersaline and acidic ponds in the Danakil Depression. However, Archaea dominated the ponds of MUP. In the current study,...

Susceptibilities of Candidatus Liberibacter asiaticus-infected and noninfected Diaphorina citri to entomopathogenic fungi and their detoxification enzyme activities under different temperatur

Abstract: Some entomopathogenic fungi species, Isaria fumosorosea, and Hirsutella citriformis were found to be efficient against the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). However, the susceptibility to these fungi increases when the psyllid infected with Candidatus Liberibacter asiaticus (Las), which is transmitted by D. citri and causes citrus greening disease. In this study, we examined the Las-infected and Las-uninfected D. citri susceptibility to entomopa...

Effects of seedling age on colonization patterns of Citrus limon plants by endophytic Beauveria bassiana and Metarhizium anisopliae and their influence on seedlings growth.

Abstract: The inoculation methods, the fungal strains, and several other factors are known to influent the success of fungal entomopathogens colonization in plants. The physiological status of the plant could also be another determinant. In the present study, the ability of three strains of Beauveria bassiana and one strain of Metarhizium anisopliae to successfully colonize Citrus limon plants and the influence of seedling age on endophytic colonization success was examined. Three, 4, and 6 ...

Susceptibility of Campylobacter strains to selected natural products and frontline antibiotics.

Abstract: Campylobacter species have developed resistance to existing antibiotics. The development of alternative therapies is, therefore, a necessity. This study evaluates the susceptibility of Campylobacter strains to selected natural products (NPs) and frontline antibiotics. Two C. jejuni strains (ATCC® 33560TM and MT947450) and two C. coli strains (ATCC® 33559TM and MT947451) were used. The antimicrobial potential of the NPs, including plant extracts, essential oils, and pure phytochem...


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