ABSTRACT 40 nasal samples were collected from apparently healthy males students at Eguare Secondary School, Ekpoma, whose ages fell within the ranges of 15-20 years were selected and screened for Staphylococcus aureus and Candida albicans using Mannitol Salt Agar and Sabouraud dextrose agar respectively. A total of 15 (37.5%) samples were positive of S. aureus while no growth was observed for Candida albicans . The disk diffusion method (Kirby-Bauer) on nutrient agar wa...
ABSTRACT The results obtained from this research work shows that land snails sold in Ekpoma market contains some pathogenic microorganisms. A total of fifty samples were bought from 10 different local sellers (five snail samples each) in Ekpoma old market and the new market. The total viable count of bacteria ranged from 2.2 x 108 cfu/g - 8.2 x 108 cfu/g. The lowest was recorded in sample I having a total bacteria count of 2.2 x 108 cfu/g and the highest count was observed in sample D obtai...
ABSTRACT Nunu is a spontaneously fermented yoghurt-like milk product consumed as a staple food commodity invirtually all parts of the Country. Its production and consumption gives way to much food security and economic benefits to the rural people. However, the process characteristics result in products which are not appealing to many people, have very short shelf-life and could have food safety concerns. In a framework of research to improve the product quality and increase consumption, a st...
ABSTRACTThe isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic media for cellulolytic count. The pour plate method was employed. Colonial morphology, gram staining and biochemical tests were used for the identificat...
ABSTRACT Microorganismsare essential players in the very existence of all living thing. They play avery important part in the production and biodegradation of organic foodsubstances in which Manihot esculentais an important player. This project evaluated the mycoflora and bacterialflora of steep cassava with the view of identifying those fungi and bacteriaspecies found with steeped cassava. This research work employed gram stainingreaction or technique and Lactophenol Cotton Blue Stain to...
ABSTRACT . Microbial quality ofcommercially prepared yoghurt sold in umuahia was evaluated. Ten(10) differentbrands of Yoghurt was purchased randomly from hawkers and beverage stores inUmuahia urban and analyzed in the laboratory. Six (6) of the samples whereregistered by NAFDAC [ Sample A, B, C, D, E and F,] while four(4) others werenot registered [ Sample G, H, I, J.]. total viable count (TVC) of the samplesranged from 3.25x102 (sample B) to 5.0x104 (sample F) forregistered samples (A t...
ABSTRACT The antibiotic susceptibility pattern of six Gram negative pathogenic bacteria; Escherichia coli, Pseudomonas, Klebsiella, Salmonella, Shigella and Enterococcus was evaluated. The test organisms were subjected to antibiotic susceptibility test using the Kirby Bauer method. A multi-disc containing eight (8) antibiotics; Ceftrazidime (30ug), Cefuroxime (30ug), Gentamicin (10ug), Ceftriaxone (30ug), Ofloxacin (5ug), Ciprofloxacin (5ug), Augmentin (30ug), Nitrofurantoin (300ug), was used...
ABSTRACT Microbial analysis was carried out on six samples of toasted bread bought from three different kiosks in Unilorin walkway. The microbial population of each sample was determined and related to a variety of physiochemical parameters that could have contributed to their population. These parameters included the pH which varied from 5.37 – 5.54, the moisture content which varied from 10% – 30%. The bacteria counts ranged from 0.3 X 105 – 4.9 X 105 cfu/g while the fungal counts ran...
antibacterial of bitter kla
ABSTRACTThe susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin, ampicillin, clarithomycin and augumentin are resistant. Escherichia coli, Klebsiella spp, and Staphylococcus aureus were sensitive to 50%, 70% and 60% ...
ABSTRACTSome locally available fruits (garden egg, orange, banana, guava, avocado, pawpaw and pineapple).sold in Oye Emene Enugu, Enugu State was examined for parasitological contamination. A total of 87 samples were examined using sedimentation and concentration methods. 11(12.6%) of the 87 fruits were positive for intestinal parasites microscopically. Among these fruits, guava had the highest number of intestinal parasites which was 6 (35.3% positive and the lowest was banana 2(8%). Parasit...
ABSTRACTSafety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green), were randomly purchased from five different vendors in Ogbete main market Enugu. The samples were transported in ice to the laboratory. The samples were bacteriologically analyzed using pour plate technique and sub-culture. Pour plate techniques was done by carrying out serial dilution of the sample after which the first tube and the last tube...
ABSTRACTA total of 50 blood (30 pregnant and 20 HIV) samples were obtained from pregnant women and PLWHA attending clinic at UNTH Ituku-ozalla. Samples were screened for hepatitis c viral infection using the rapid one step hepatitis C virus test strip. Two (6.66%) pregnant women were positive and four (20%) HIV patients were positive for Hepatitis C, giving an overall prevalence rate of (26.7%). The infection was the same in male (3) and in female (3). Those aged 23-34 years recorded a higher...
ABSTRACTEffect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. ...
ABSTRACTComparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determine their levels of contamination of bacteria. The sources of water examined are tap water, well water and stream water. The pour plate method was used to examine the water samples. Bacteria isolated from the water samples include coliforms especially Escherichia coli. Stream water and well water were found to contain bacterial isolates unlike the tap water t...