Cross-species hybridization approaches have been used for genetic analysis of species
that do not have complete genome arrays available. The study used the cross-species
DNA-DNA hybridization approach to characterize Ximenia americana biological
processes. Preliminary micro-array studies done in the Department of Chemistry and
Biochemistry at the University of Namibia has created X. americana DNA-DNA
hybridization to a well-known Arabidopsis thaliana genechip (ATH1). A. thaliana
probe-pairs that hybridised to the X. americana genomic DNA on the basis of the
perfect-match (PM) probe signal were selected and analysed using a cel file parser script
to generate a new high density probe mask files. These files effectively represent the
first ever X. americana DNA-DNA hybridisation data. This study conducted gene
ontology analysis of X. americana/A. thaliana hybridisation data. Such gene ontology
analysis demonstrated that abiotic stress response genes are over-represented in relative
comparison to model species A. thaliana under natural conditions. This observation was
independently confirmed with PCR amplification of the following orthologous genes
using X. americana genomic DNA: AT4G15910.1, SAD2, HXK1, ACC and ERF/AP2.
Given the lack of genomic sequence information in X. americana background, primers
for genomic amplification was design using A. thaliana genomic sequence information.
Primers where designed to yield 100 bp genomic PCR product. Each of the selected
genes was successfully amplified hence giving evidence of homology within primer
binding sites. Nonetheless, the genomic amplification of these key abiotic factors in X.
americana confirms the type of responses that supports the adaptation of X. americana
under natural conditions that are stress related (heat and drought stress). Although
transcript levels of this important abiotic response factors could not be measured in
absolute or relative terms, the study demonstrated inherent presence of such genes at the
genomic level of X. americana. Further studies will be required to demonstrate that
some sort of modification of any of these or other abiotic response factors within the
genome of X. americana provides the key basis for its adaptation to the relatively dry
and hot climatic conditions.
SSA, R (2021). Genetic Analysis Of Ximenia Americana Under Natural Conditions. Afribary.com: Retrieved May 10, 2021, from https://afribary.com/works/genetic-analysis-of-ximenia-americana-under-natural-conditions
Research, SSA. "Genetic Analysis Of Ximenia Americana Under Natural Conditions" Afribary.com. Afribary.com, 27 Apr. 2021, https://afribary.com/works/genetic-analysis-of-ximenia-americana-under-natural-conditions . Accessed 10 May. 2021.
Research, SSA. "Genetic Analysis Of Ximenia Americana Under Natural Conditions". Afribary.com, Afribary.com, 27 Apr. 2021. Web. 10 May. 2021. < https://afribary.com/works/genetic-analysis-of-ximenia-americana-under-natural-conditions >.
Research, SSA. "Genetic Analysis Of Ximenia Americana Under Natural Conditions" Afribary.com (2021). Accessed May 10, 2021. https://afribary.com/works/genetic-analysis-of-ximenia-americana-under-natural-conditions