Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS), which are involved in the pathogenesis of oxidative stress-related disorders like diabetes, nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological disorders when they attack biochemical molecules like proteins, lipids and nucleic acid. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Natural based antioxidants such as catalase, glutathione, superoxide dismutase, vitamin C and E in the body are known to quench free radicals. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemiclas are currently pursued as an alternative and complimentary drug. Strychnos henningsii and Ficus sycomorous are used in Africa as traditional medicine for treatment of various ailments including rheumatism, syphilis, gastro intestinal pain, snake bites, abdominal pain diabetes as well as anaesthology, healing of wounds and as a mouth antiseptic. This study was designed to evaluate in-vitro antioxidant activities of DCM leaf extract of S. henningsii and stem bark extract of F. sycomorus using 1,1-diphenly-2picrylhydrazyl (DPPH), Hydrogen peroxide and Ferric reducing power assays. The antioxidants activities were assayed against ascorbic acid as a reference drug. Total flavonoids and phenolic compound were determined by Folin–Ciocalteu reaction. The results obtained showed both extracts significantly (p<0.05) exhibited antioxidants activities at different concentrations tested. The DCM leaf extract of S. henningsii and stem bark extract of F. sycomorus scavenge hydrogen peroxide radicals (H2O2) in a dose dependent manner. The half maximal percentage inhibition (IC50) of S. henningsii and F. sycomorus was 0.325 mg/ml and 0.330 mg/ml, respectively. The two extracts also scavenged DPPH in a dose dependent manner. Their IC50 value was 0.068mg/ml for S. henningsii and 0.062mg/ml for F. sycomorus. Both DCM leaf and stem bark extract of S. henningsii and F. sycomorus were found to have strong ferric reducing power in a dose dependent manner. Both the total phenolic and flavonoid content of DCM stem bark extract of F. sycomorus was lower than the leaf extract of S. henningsii. Preliminary phytochemical screening showed that both extracts possess saponins, flavonoids, phenols, steroids, alkaloids and cardiac glycoside however terpenoid were found to be absent in S. henningsii. Therefore, the results in this study showed both extracts possess secondary metabolites which are associated with antioxidants activities. The present study therefore recommends a further screening to enhance their ultimate application in management of oxidative stress-related disorders.
AfroAsia, R (2021). In Vitro Antioxidant Activities Of Dichloromethane Extracts Of Strychnos Henningsii G. And Ficus Sycomorus L.. Afribary.com: Retrieved June 18, 2021, from https://afribary.com/works/in-vitro-antioxidant-activities-of-dichloromethane-extracts-of-strychnos-henningsii-g-and-ficus-sycomorus-l
Research, AfroAsia. "In Vitro Antioxidant Activities Of Dichloromethane Extracts Of Strychnos Henningsii G. And Ficus Sycomorus L." Afribary.com. Afribary.com, 06 Jun. 2021, https://afribary.com/works/in-vitro-antioxidant-activities-of-dichloromethane-extracts-of-strychnos-henningsii-g-and-ficus-sycomorus-l . Accessed 18 Jun. 2021.
Research, AfroAsia. "In Vitro Antioxidant Activities Of Dichloromethane Extracts Of Strychnos Henningsii G. And Ficus Sycomorus L.". Afribary.com, Afribary.com, 06 Jun. 2021. Web. 18 Jun. 2021. < https://afribary.com/works/in-vitro-antioxidant-activities-of-dichloromethane-extracts-of-strychnos-henningsii-g-and-ficus-sycomorus-l >.
Research, AfroAsia. "In Vitro Antioxidant Activities Of Dichloromethane Extracts Of Strychnos Henningsii G. And Ficus Sycomorus L." Afribary.com (2021). Accessed June 18, 2021. https://afribary.com/works/in-vitro-antioxidant-activities-of-dichloromethane-extracts-of-strychnos-henningsii-g-and-ficus-sycomorus-l