ABSTRACT
Waste generated by several agricultural activities has many alternative uses. Unfortunately, much of it is burnt in the open fields, resulting in environmental pollution. In view of the economic and ecological importance of agro wastes, microfungi were isolated from decaying agro wastes samples and screened for pectinolytic activity using a plate assay method containing pectin alone. The effects of carbon, nitrogenous sources and pH on pectinase production by the strains with maximum pectinolytic activities were investigated. Hyper- production of pectinase by these strains using ultra-violet irradiation (UV) as mutagen was also investigated. The selected mutants of Penicillium chrysogenum and Aspergillus niger were characterized using catabolite repression. The genetic variability between the wild and mutant strains was investigated using Random Amplified Polymorphic DNA (RAPD) primers. The polygalacturonase (Pga) produced by the mutant strains were partially purified and the enzyme properties were investigated. Five fungi strains were isolated they are: Aspergillus niger, Aspergillus clavetus, Penicillium chrysogenum, Fusarium species and Trichoderma harzianum. Pectinase production by these isolates showed that the highest pectinase activity index was observed in Aspergillus niger (A.n) followed by Penicillium chrysogenum (P.C). The growth profile of the A.n and P.c in various agro wastes studied showed that these fungi were able to grow in both simple sugar (metabolites) and various agro wastes.
ANTHONY, O (2021). Isolation And Molecular Characterization Of Pectinase From Penicillium Chrysogenum And Aspergillus Niger Grown On Some Agro Wastes In Lagos Nigeria. Afribary. Retrieved from https://afribary.com/works/isolation-and-molecular-characterization-of-pectinase-from-penicillium-chrysogenum-and-aspergillus-niger-grown-on-some-agro-wastes-in-lagos-nigeria
ANTHONY, OKAFOR "Isolation And Molecular Characterization Of Pectinase From Penicillium Chrysogenum And Aspergillus Niger Grown On Some Agro Wastes In Lagos Nigeria" Afribary. Afribary, 08 May. 2021, https://afribary.com/works/isolation-and-molecular-characterization-of-pectinase-from-penicillium-chrysogenum-and-aspergillus-niger-grown-on-some-agro-wastes-in-lagos-nigeria. Accessed 27 Apr. 2024.
ANTHONY, OKAFOR . "Isolation And Molecular Characterization Of Pectinase From Penicillium Chrysogenum And Aspergillus Niger Grown On Some Agro Wastes In Lagos Nigeria". Afribary, Afribary, 08 May. 2021. Web. 27 Apr. 2024. < https://afribary.com/works/isolation-and-molecular-characterization-of-pectinase-from-penicillium-chrysogenum-and-aspergillus-niger-grown-on-some-agro-wastes-in-lagos-nigeria >.
ANTHONY, OKAFOR . "Isolation And Molecular Characterization Of Pectinase From Penicillium Chrysogenum And Aspergillus Niger Grown On Some Agro Wastes In Lagos Nigeria" Afribary (2021). Accessed April 27, 2024. https://afribary.com/works/isolation-and-molecular-characterization-of-pectinase-from-penicillium-chrysogenum-and-aspergillus-niger-grown-on-some-agro-wastes-in-lagos-nigeria