ABSTRACT
Ten phages were isolated randomly from sewage sources and
disposal points on the University of Ghana,Legon campus, purified and
characterised as delivery systems for the resistance marker genes,
Strr and Benr. The phages were found to have proteins of relative
molecular weights ranging from 9,000 - 100,000. Eight phages were
found to be morphologically related to the tailed phages. The other
two were tailless phages. All the coliphages isolated were
morphologically related to the T-even phages, whilst Sf RBCL 15 and
Sd RBCL 5 were related to the P-phages. The tailless phages were
found to be morphologically related to the p phages (06 and 0X174
phages). The coliphages were found to be closely related to each
other. They did exhibit some detectable cross reactivity to Sd RBCL
5, Sd RBCL 2 3 and Sf RBCL 15. The coliphages were not related to the
S. tvphi phages.
Selected pathogenic bacteria from the Noguchi Memorial Institute
for Medical Research and the Medical School of the university of
Ghana were screened for the presence of the antibiotic resistance
gene marker. All the bacteria, except three, were found to be highly
resistant to the marker antibiotics used : Salmonella Group D was
relatively more sensitive to all three antibiotics ; Staphylococcus
aureus was sensitive to Benzylpenicillin and Salmonella typhi was
sensitive to Tetracycline.
The isolated phages with the exception of St RBCL 2 0 exhibited
the ability to transduce the resistance marker genes from one
bacterium to sensitive bacteria at a high frequency.
OWUSU-BINEY, A (2021). Phage - Mediated Transfer Of Genetic Material. Afribary. Retrieved from https://afribary.com/works/phage-mediated-transfer-of-genetic-material
OWUSU-BINEY, ALEX "Phage - Mediated Transfer Of Genetic Material" Afribary. Afribary, 08 Apr. 2021, https://afribary.com/works/phage-mediated-transfer-of-genetic-material. Accessed 15 Nov. 2024.
OWUSU-BINEY, ALEX . "Phage - Mediated Transfer Of Genetic Material". Afribary, Afribary, 08 Apr. 2021. Web. 15 Nov. 2024. < https://afribary.com/works/phage-mediated-transfer-of-genetic-material >.
OWUSU-BINEY, ALEX . "Phage - Mediated Transfer Of Genetic Material" Afribary (2021). Accessed November 15, 2024. https://afribary.com/works/phage-mediated-transfer-of-genetic-material