ABSTRACT
Alcoholic extract of Croton membranaceus Mull. Arg is used at the Centre for Scientific Research into Plant Medicine at Mampong Akwapim to treat retention of urine due to benign prostatic hyperplasia (BPH). The effect of the extract on rat steroid 5areductase activity in invo was investigated using a radioimmunoassay technique to quantitate dihydrotestosterone (DHT) levels in blood. The effects of the extract and two unidentified alkaloidal isolates of the plant, provisionally referred to as CM-1 and CM-3, were investigated in vitro as potential inhibitors of 5a-reductase activity using a thin layer chromatographic technique to quantitate DHT formed from testosterone. The results show that the extract may not lower rat serum DHT levels significantly in vivo but both the extract and the isolated CM-1 inhibited the enzyme activity in vitro at the concentrations used. The other isolate, CM-3, did not inhibit the enzyme at the concentration used. The results obtained suggest that inhibition of 5a-reductase activity may not be the major mechanism by which the extract exerts its action in BPH patients.
ABOAGYE, F (2021). The Effect of Croton Membranaceus on DIHYDROTESTOSTERONE Levels in Blood And its Synthesis by Prostatic 5a-Redutase in The Rat. Afribary. Retrieved from https://afribary.com/works/the-effect-of-croton-membranaceus-on-dihydrotestosterone-levels-in-blood-and-its-synthesis-by-prostatic-5a-redutase-in-the-rat
ABOAGYE, FREDERICK "The Effect of Croton Membranaceus on DIHYDROTESTOSTERONE Levels in Blood And its Synthesis by Prostatic 5a-Redutase in The Rat" Afribary. Afribary, 17 Apr. 2021, https://afribary.com/works/the-effect-of-croton-membranaceus-on-dihydrotestosterone-levels-in-blood-and-its-synthesis-by-prostatic-5a-redutase-in-the-rat. Accessed 25 Apr. 2024.
ABOAGYE, FREDERICK . "The Effect of Croton Membranaceus on DIHYDROTESTOSTERONE Levels in Blood And its Synthesis by Prostatic 5a-Redutase in The Rat". Afribary, Afribary, 17 Apr. 2021. Web. 25 Apr. 2024. < https://afribary.com/works/the-effect-of-croton-membranaceus-on-dihydrotestosterone-levels-in-blood-and-its-synthesis-by-prostatic-5a-redutase-in-the-rat >.
ABOAGYE, FREDERICK . "The Effect of Croton Membranaceus on DIHYDROTESTOSTERONE Levels in Blood And its Synthesis by Prostatic 5a-Redutase in The Rat" Afribary (2021). Accessed April 25, 2024. https://afribary.com/works/the-effect-of-croton-membranaceus-on-dihydrotestosterone-levels-in-blood-and-its-synthesis-by-prostatic-5a-redutase-in-the-rat