Chlorpyrifos [CPF; O,O-diethyl O-(3,5,6-trichloro-2-pyridinyl) phosphorothionate] is a widely used organophosphate pesticide that is known to be toxic to the environment and to living beings. It affects several organs of the body especially the brain leading to impairment of normal functioning of the body. Diphenyl diselenide (DPDS), an organsoselenium compound is the simplest of the synthetic diaryldiselenides. Studies have shown that DPDS exhibits anti-inflammatory and antioxidant effects if administered in pharmacological doses. This present study was aimed at investigating the neurotoxic effects of CPF on the midbrain of male Wistar rats and the possible counter effects by DPDS.
This project work was carried out using a total of sixty (60) male Wistar rats which were grouped into five cages of twelve rats each. The animals underwent acclimatization before being treated for 5 weeks. Group A rats were given corn-oil as control; Group B rats received DPDS (5mg/kg) only dissolved in corn-oil; Group C rats were treated with CPF (5mg/kg) only dissolved in corn-oil; Group D rats were co-exposed to CPF (5mg/kg) + DPDS (2.5mg/kg) while Group E rats were co-exposed to CPF (5mg/kg) + DPDS (5mg/kg) for the whole of the 5 weeks after which they were euthanized. The mid-brain was excised, homogenized and centrifuged. Markers of brain oxidative damage and histopathology were evaluated in the rats.
Superoxide dismutase (SOD) activity, catalase (CAT) activity, reduced glutathione (GSH) level, Glutathione Peroxidase (GPx) activity, glutathione-S-transferase (GST) activity and acetylcholinesterase (AChE) activity were significantly(p < 0.05) reduced in the mid-brain of the rats treated with chlorpyrifos (CPF) alone as compared to the control rats. Also, oxidative and inflammatory markers such as hydrogen peroxide (H2O2) level, lipid peroxidation (LPO) and myeloperoxidase (MPO) activity respectively were significantly increased in the same rats. However, the co-exposure with DPDS ameliorated the neurotoxic effects of CPF by increasing the antioxidant level and suppressing the oxidative stress biomarkers. Also, the weights of the midbrain of rats treated with CPF showed no significant change.
Summarily, DPDS reversed the neurotoxic effects in midbrain induced by CPF via the antioxidant and anti-inflammatory properties which it possesses.
Adeigbe, V (2020). CHEMOPROTECTIVE ROLES OF DIPHENYL DISELENIDE(DPDS) ON CHLORPYRIFOS(CPF)- INDUCED NEUROTOXICITY IN MID-BRAIN OF MALE RATS. Afribary.com: Retrieved November 27, 2020, from https://afribary.com/works/adeigbe-victors-project-work-feb-2018
Victor, Adeigbe. "CHEMOPROTECTIVE ROLES OF DIPHENYL DISELENIDE(DPDS) ON CHLORPYRIFOS(CPF)- INDUCED NEUROTOXICITY IN MID-BRAIN OF MALE RATS" Afribary.com. Afribary.com, 18 Jun. 2020, https://afribary.com/works/adeigbe-victors-project-work-feb-2018 . Accessed 27 Nov. 2020.
Victor, Adeigbe. "CHEMOPROTECTIVE ROLES OF DIPHENYL DISELENIDE(DPDS) ON CHLORPYRIFOS(CPF)- INDUCED NEUROTOXICITY IN MID-BRAIN OF MALE RATS". Afribary.com, Afribary.com, 18 Jun. 2020. Web. 27 Nov. 2020. < https://afribary.com/works/adeigbe-victors-project-work-feb-2018 >.
Victor, Adeigbe. "CHEMOPROTECTIVE ROLES OF DIPHENYL DISELENIDE(DPDS) ON CHLORPYRIFOS(CPF)- INDUCED NEUROTOXICITY IN MID-BRAIN OF MALE RATS" Afribary.com (2020). Accessed November 27, 2020. https://afribary.com/works/adeigbe-victors-project-work-feb-2018