Evaluation of the viability and survival rates of some selected pathogens

Artificially inoculated fruit juice is a very viable profit making sole business in Nigeria, the product is locally available in all market in different parts of the country, especially in Ondo state, using Ikare and Iwaro Akoko market, as the focal point of this present study. Fifty (50) samples of the inoculated fruit juice were purchase from the market in Iwaro and Ikare Akoko, Ondo State. The test organisms used for this research work are Staphylococcus aureus (ATCC) 25923, Salmonella spp (ATCC) 43971, and Escherichia coli 0157:H7 (ATCC) 27325, Pseudomonas aeruginosa (ATCC) 9027 and Aspergillus flavus (ATCC) 9804, 83435. Sterility test and survival rate of the test organisms were carefully carried out into the inoculated fruit juice which were purchase from the two market. It was observed that the survival rate of Staphylococcus aureus (ATCC) 25923, Pseudomonas aeruginosa (ATCC) 9027, Escherichia coli 0157:H7 (ATCC) 27325, Salmonella spp (ATCC) 43971 and Aspergillus flavus (ATCC) 9804, 83435 were conspicuously very high, Staphylococcus aureus(ATCC) 25923 were completely inhibited within ten(10) hours contact time and none of these microbes were viable for a period of hundred (100) hours in the fruit juices with preservatives except Aspergillus flavus (ATCC) 9804, 83435. It was also observed that the viability of of Aspergillus flavus (ATCC) 9804, 83435 may be due to presence of spores formation within the inoculated fruit juice. The purpose of this present study is to investigate the viability and survival rates of the microorganism in artificially inoculated fruit juice in Iwaro and Ikare market of Ondo state, Nigeria