Research Papers/Topics in Biochemistry

Effect of Chronic Exposure to Petroleum Hydrocarbon Pollution On Some Biochemical Parameters of Individuals Native to Ebocha, Niger Delta

ABSTRACT The impact of pollution due to gas flaring on individuals native to Ebocha in Niger Delta Area was investigated. One hundred and five apparently healthy individuals between 21-70years who consented for the study were screened, seventy five individuals from Ebocha and thirty from Nsukka-a control location with no history of petroleum hydrocarbon pollution. Standard analytical procedures were used in the determination of the concentration of ascorbic acid (AA) of the individuals (males...

Chemo protective Effect of Methanol Leaf Extract of Picralima Nitida On Carbon Tetrachloride-Induced Oxidative Stress in Albino Rats

ABSTRACT Among medicinal plants, Picralima nitida is well investigated and has been shown to possess renal and hepatoprotective activities. The present study was conducted to investigate the chemoprotective effect of methanol extract of Picralima nitida leaves on carbon tetrachloride-induced oxidative stress in albino rats. Thirty (30) Wistar albino rats of six (6) rats per group were used for this study and were divided into 5 groups. Group 1 rats were normal control; group 2 (negative contr...

Assessment of Phytochemical and Nutrient Compositions of Edible Yam Species in Southeastern Nigeria

ABSTRACT This study assessed the phytochemical and nutrient compositions of edible yam species Dioscorea rotundata 1, Dioscorea rotundata 2, Dioscorea rotundata 3, Dioscuri rotundata 4, Dioscorea Cayenensis, Dioscorea alata, Dioscorea dumentorum and Dioscorea bulbifera in Southeastern Nigeria. The phytochemical and nutrient (proximate, minerals, vitamins, fatty acids and amino acids) compositions of the yam tubers were investigated using standard analytical methods. 

Diagnostic Potential of Extant Antischistosoma Genus-Specific Monoclonal Antibodies

ABSTRACT The suitability o f five Schistosoma genus-specific monoclonal antibodies (MoAbs) (Sh3/34.10, Sh3/38.2, Sh4/14.3, Sh5/32.30 and Sh5/34.10) in detecting schistosome antigens in infected human and cattle was evaluated. These antibodies were employed in various diagnostic assays to diagnose human and animal schistosomiasis. Extant MoAb secreting hybridoma cells were first propagated in culture to produce the MoAbs. The culture supernatants containing secreted antibodies were analysed b...

The Effects of Cryptolepis Sanguinolenta Root Extracts On Plasmodium Falciparum Gametocyte Development

ABSTRACT Current efforts to eliminate malaria worldwide are soaring and one of the approaches to this is the mass screening of medicinal plants for their possible potential anti-malarial property. The current study conducted was to determine the effect of one such plant, Cryptolepis sanguinolenta on gametocyte development. In vitro growth inhibition assays on early and late gametocytes stage using ethanolic and aqueous extract of the C. sanguinolenta roots was performed. Enzyme activity assa...

Isolation and Characterization of Antifungal Agents Produced by Wood Decaying Fungi from Ghana

ABSTRACT Human systemic fungal infections have increased in the past decade due to the dramatic increase in immunocompromised patients hence the emergence of resistant fungal strains. It is therefore necessary to discover new antifungal compounds with novel mode of action. This study was aimed at isolating and characterizing antifungal agents from wood decaying fungi (WDF). The growth conditions for maximum production of bioactive agents from WDF were first tested. It was observed that minera...

Antimalarial And Antioxidant Effects Of Methanol And Flavonoid-Rich Extracts Of Adansonia Digitata Stem Bark On Plasmodium Berghei-Infected Mice

ABSTRACT Adansonia digitata has popular ethnomedicinal application in the treatment of malaria in sub-Saharan Africa. The present study sought to investigate the antimalarial and antioxidant effects of methanol and flavonoid-rich extracts of the stem bark on Plasmodium berghei-infected mice in vivo. Thirty-five male mice, weighing 18-20 g and randomly allocated into seven groups of five animals each were used. Group 1, which served as the positive control, was pretreated with 1 ml/kg of...

Influence Of Gallic Acid On Α-Amylase And Α-Glucosidase Inhibitory And Antioxidant Properties Of Acarbose

ABSTRACT Type 2 diabetes mellitus (T2DM) is a chronic progressive disease that has continued to be a global heath and economic burden. Acarbose is an antidiabetic drug, which acts by inhibiting alpha amylase and alpha glucosidase; while gallic acid is a simple phenolic acid that is widespread in plant foods and beverages such as tea and wine.This study therefore, sought to investigate the influence of gallic acid on α-amylase and α-glucosidase inhibitory and antioxidant properties of ...

Toxicity Of Single And Quatenary Mixture Of Antibiotics To Natural Microbial Community.

ABSTRACTThe toxicities of single antibiotics: ciprofloxacin, azithromycin, sulfathiazole and amoxicillinand their quaternary mixtures in different ratios were examined on three organismsStaphylococcus aureus, Bacillus cereus and Micrococcus luteus all isolated from Nworie Riverfrom three different points including the point of effluent discharge of a hospital. Toxicity ofantibiotics and mixtures was assessed using inhibition of total dehydrogenase assay method.Result obtained from the study s...

Effects of Extracts of The Anti-Asthmatic Plant Thonninga Sanguinea on Anaphylaxis in The Guinea Pig

ABSTRACT Thonningia sanguinea is one of the herbal preparations vised prophylactieally against asthma at the Centre for Scientific Research into Plant Medicine. Preparations of this plant are used with Desmodium adseendens which has been shown to be anti-anaphylactic. The anaphylactic reaction is a basic underlying phenomenon in asthma. In this work, the effects of T. eanguinea preparations were evaluated on some aspects of the disease. The method adopted was experimental anaphylaxis in...

Antidiabetic Properties of Total Phenol Content of Zanthoxylum zanthoxyloides Leaves on Albino Rats

ABSTRACT    In this study, we evaluated the effect of the total phenol extract of Zanthoxylum zanthoxyloides leaves on oral glucose tolerance, lipid profile, liver function, oxidative stress parameters, and pancreas histopathology in diabetic rats. Diabetes was induced in rats by administration of alloxan monohydrate in a dose of 130 mg/kg b/w. Total phenol content of Z. zanthoxyloides leaves was administered to rats at 100, 200 and 400 mg/kg b/w daily for 15 days. Blood gluco...

THE EFFECT OF TEMPERATURE ON GLUTATHIONE PEROXIDASE FROM FISH

Glutathione Peroxidase (GPx) family of enzymes plays important roles in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. This study was conducted to investigate the possible effects of temperature in vivo on Glutathione peroxidase (GPX) oxidative activity in fish tissues.An adult fully grown cat fish, (the African flath...

ANTI-TRYPANOSOMAL ACTIVITY OF N-HEXANE AND DICHLOROMETHANE EXTRACTS OF VITELLARIA PARADOXA LEAVES ON TRYPANOSOMA BRUCEI BRUCEI INFECTED MICE

African trypanosomosis is a vector-borne disease that is caused by a flagellated protozoon of the genus Trypanosoma. Large populations of human and domestic animals are at risk of infections with trypanosomes in vast areas of sub-Saharan Africa where the disease is endemic. Human infections with trypanosomes are referred to as Human African Trypanosomosis (HAT) while animal infections are known as African Animal Trypanosomosis (AAT). These infections are more prevalent in the rural areas (Ato...

Investigation of the Pancreatic Effect of Buccholzia Coricacea Formulated Diet in Sucrose Fed Pregnant Rats and their Offspring

ABSTRACTSeveral plants have been used in the treatment of various disorders without scientific basis. The present study examine the pancreatic effect of Buchholzia coriacea formulated diet in sucrose-fed pregnant rats and their offspring. Buchholzia coriacea seeds were obtained from Ogbete main market, Enugu state, identified, dried and grounded using a miller machine. 10% of the seed powder was used to formulate their diet. Thirty (240) adult female and six (6) adult male albino rats (180-25...

Hypolipidemic and Antioxidant Capacity of Methanol Leaf Extract of Kigelia Africana in Alloxan Induced Diabetic Albino Rats

ABSTRACTDiabetic mellitus is a metabolic disorder resulting from a defect in insulin secretion , insulin action or both . The deficiency of insulin leads to chronic hyperglycemia with disturbances of carbohydrate , fats  and protein metabolism. Kigelia Africana is a sausage tree known to have medicinal values. The aim of this work is to evaluate the hypolipidemic and antioxidant capacity of the plant , methanol leaf extract of kigelia Africana which was used for the study. The leaves wer...


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