Research Papers/Topics in Biochemistry

Biochemical Diversity Of Tea Germplasm And Potential For Processing Of Diversified Tea Products

ABSTRACT Tea industry in Kenya forms the largest agribusiness contributing up to 4% of the country’s gross domestic product and over 26% of total foreign exchange earnings. However, significant revenue is lost when tea is sold in undiversified form. Therefore, this study aimed at determining the biochemical diversity in Kenyan tea clones and their potential for diverse product development. Samples were obtained from 197 tea clones conserved in Kericho and Kangaita substation and assayed fo...

Characterization And Antimicrobial Activity Of Tropical Basidiomycetes Fungi And Their Secondary Metabolites

ABSTRACT Microbial infections remain to be a major global public health challenge. In Kenya, these infections cause significant morbidity and mortality. Their devastating effects are majorly attributed to antimicrobial drug resistance. Nonetheless, the biodiversity and ecology of tropical Basidiomycetes fungi is an untapped source of potential bioactive compounds. Therefore, the current study aimed to isolate and test crude extracts and antimicrobial compounds from Basidiomycetes against sel...

Chemical Compositions And Antimicrobial Activity Of Amaranthus Hybridus, Amaranthus Caudatus, Amaranthus Spinosus And Corriandrum Sativum.

ABSTRACT Different communities in Kenya use a wide variety of indigenous plants for food. About 850 species of plants are used for food. To this group belong Amaranthus species that has important nutrients and a rich array of biologically active secondary metabolites. Corriandrum sativum is a leafy spice/ herb that contain volatile oils from which it derives its flavour and aroma and also antimicrobial properties. Essential oils from fresh leaves of C. sativum were extracted by hydro distill...

Induced Proteins Profile In The Haemolymph Of Desert Locust (Schistocerca Gregaria) Following A Trypanosomatid Flagellate (Trypanosoma Brucei Brucei) Challenge

ABSTRACT Innate immunity has a key role in the control of microbial infections in both vertebrates and invertebrates. In insects, including vectors that transmit parasites that cause major diseases such as trypanosomosis, leishmaniasis and filariasis, antimicrobial peptides and agglutinins form an important component of innate immunity and participate in regulating parasite development. In this study, induced haemolymph peptides from a non-vector, nonheamatophagous insect, Schistocerca grega...

Analysis Of Total Hla-G Levels And Its Isoforms In Placental Malaria

ABSTRACT The present study examined the total, membrane bound and soluble HLA-G in infected and uninfected placentas using quantitative real time PCR, ELISA and immunohistochemistry. The total HLA-G transcripts differed significantly (p = 0.009) between infected and uninfected primigravidae. The membrane bound HLA-G transcripts were significantly more abundant than the soluble HLA-G in infected placentas (p = 0.04) in all mothers. At the protein level infected placentas had higher mean level...

Nutritional Characterisation Of Baobab (Adansonia Digitata L.) Fruits Based On Africa Geographical Regions

ABSTRACT Baobab (Adansonia digitata L.) is an indigenous fruit tree occurring in the savannah drylands of sub-Saharan Africa. A vast population of people in this region suffers from hidden hunger and malnutrition. Baobab fruits are a source of micronutrients, and their utilization in local diets has potential to improve health of the rural poor. The present study was conducted to determine the nutritional characteristics of baobab pulp and seeds across Africa geographical regions. Mature baob...

Determination Of Benzo[A]Pyrene Levels And Establishment Of Limit Of Detection In Smoked And Oil Fried Lates Niloticus

ABSTRACT Polycyclic aromatic hydrocarbons (PAHs) are a large group of organic compounds included in the European Union and US Environmental Protection Agency (USEPA) priority pollutant list because of their mutagenic and carcinogenic properties. Several studies have shown that exposure to benzo[A]pyrene (BaP), a member of the PAHs increases the risk of cancer. PAHs have been the subject of much concern in the recent years due to their toxic potential. They are known as highly stable contamina...

Antidiabetic And Wound Healing Properties Of Selected Kenyan Tea Cultivars

ABSTRACT Diabetes mellitus is a metabolic disorder of multiple etiology, characterized by chronic hyperglycemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action or both. It is associated with significant morbidity and mortality due to cardiovascular complications as a result of imbalance in lipid metabolism. Diabetic foot ulcers (DFUs) are estimated to occur in 15% of all patients with diabetes and precede 84% of all dia...

Determination Of Antioxidant And Metal Chelating Properties Of Tea (Camelia Sinensis) In Ameliorating Cadmium Induced Toxicity In Male Wistar Ratsc

ABSTRACT Cadmium (Cd) is a ubiquitous environmental and industrial pollutantwhose exact toxicity mechanisms still remain elusive. However, cumulative data has implicated bioaccumulation as well as free radical generation in the biochemical and molecular mechanisms of cadmium induced toxicity. Chelation therapy with calcium disodium ethylenediamine tetra acetic acid (CaNa2EDTA), British Anti Lewisite (BAL), meso 2, 3-dimercaptosuccinic acid (DMSA) is so far among the best known treatment again...

Production Of Infective Trypanosoma Brucei Brucei Expressing Green Fluorescent Protein For Localisation Of Trypanosomes In Brain Of Infected Rodents

ABSTRACT Human African trypanosomosis (HAT) is caused by subspecies of T. brucei found in East, Central and West Africa, with T. b. rhodesiense and T. b .gambiense causing sleeping sickness in human through the bite of a tsetse fly of the genera Glossina. The treatment of CNS infection is difficult and at the same time the biology of late stage trypanosome infection is poorly understood. In addition, the parasites hideout in the brain and their mode of survival and multiplication in the brain...

Development Of A Novel Dna Isolation Protocol And Determination Of The Transmission Mode Of Banana Streak Virus By Putative Vectors In Kisii

ABSTRACT Banana is an important crop in East Africa. In the tropics, banana and plantains are a major source of carbohydrate for about 400 million people of whom 200 million are from East Africa. In Kenya, the banana serves both as a subsistence crop as well as a cash crop. The production of banana in Kenya is however constrained by viral diseases especially the Banana Streak Disease (BSD) which is caused by the Banana Streak Virus (BSV). Breeding between A and B-genomes containing banana ha...

Determination Of Antibacterial Activity Of Extracts From Markhamia Lutea, Zanthoxylum Gilletii And Their Endophytes Against Common Bean Bacterial Pathogens

ABSTRACT Common bean (Phaseolus vulgaris L.) is largely consumed among various communities in Kenya. However, its productivity is gradually declining due to infections such as common bacterial blight and halo blight. Zanthoxylum gilletii and Markhamia lutea have been used traditionally in the management of various human bacterial pathogens. In addition, plants are inhabited by fungal endophytes that produce bioactive secondary metabolites. This study therefore, was to determine the bioactivit...

Purification And Characterization Of A Cyclooxygenase-Like Enzyme From Trypanosoma Brucei

 ABSTRACT The protozoan hemoflagellate Trypanosoma brucei is the causative agent of African trypanosomosis in humans and nagana in domestic animals. The human disease is a health concern in many African countries. The infection of mammalian-host by African trypanosomes is characterised by an up-regulation of prostaglandin (PG) production in the plasma and cerebrospinal fluid (CSF), which modulates the host responses and causes symptoms of the infection. It has been shown that the protozoan ...

Molecular Characterisation Of Tsetse-Endosymbiont Interactions In Kenyan Populations Of Glossina Austeni And Glossina Pallidipes Tsetse Flies

ABSTRACT Tsetse flies (Diptera: Glossinidae), which are the major vectors of human and animal trypanosomes, harbour bacterial gut symbionts Wigglesworthia glossinidia, Wolbachia pipientis and Sodalis glossinidius in close association with trypanosomes. The objective of this study was to determine the prevalence, temporal variation and strain diversity of these endosymbionts in G. austeni and G. pallidipes from the Kenyan coast. The co-relation between symbiont and trypanosomes infection wa...

Determination Of Antioxidant And Metal Chelating Properties Of Tea (Camelia Sinensis) In Ameliorating Cadmium Induced Toxicity In Male Wistar Rats.

ABSTRACT Cadmium (Cd) is a ubiquitous environmental and industrial pollutantwhose exact toxicity mechanisms still remain elusive. However, cumulative data has implicated bioaccumulation as well as free radical generation in the biochemical and molecular mechanisms of cadmium induced toxicity. Chelation therapy with calcium disodium ethylenediamine tetra acetic acid (CaNa2EDTA), British Anti Lewisite (BAL), meso 2, 3-dimercaptosuccinic acid (DMSA) is so far among the best known treatment again...


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