Microbiology Research Papers/Topics

Occurrence and Multiple Antibiotic Susceptibility Profile of Bacterial Pathogens Isolated from Patients with Chronic Respiratory Diseases

Abstract Objectives: The present study reported the antibiotic susceptibility profile and pattern of pathogens isolated from in- and out-patients with respiratory tracts infections. Methods: Sputum and throat swab samples were collected aseptically from subjects and cultured on the appropriate bacteriological media. Bacterial isolates were identified using biochemical tests and the identity of the isolate was later confirmed using Analytical Profile Index 20E kit. Antimicrobial susceptibilit...

Evaluation of Inhibitory Zone Diameter (IZD) of crude Spondias mombin (Linn.) extracts (root, leaf, and stem bark) against thirty infectious clinical and environmental isolates.

Abstract The purpose of this work is to evaluate the Inhibitory Zone Diameter (IZD) of crude Spondias mombin extracts against thirty infectious clinical and environmental organisms. The root, leaf and stem-bark of S. mombin were harvested and air-dried. The dried S. mombin was milled into powdered form using manual grinder. Powdered S. mombin (1kg) each of the different S. mombin parts was extracted with 3 L of 70% (v/v) ethanol, ethyl acetate and distilled water for 72 h at room temperature....

Chewing Sticks and Oral Health Care in Owo Local Government, Ondo State

-the aim of this study is to carried out the Antimicrobial and phytochemical effect on five medicinal plants used as chewing sticks in Owo local government of Ondo State, Nigeria. Methods- The methods used for this study are antimicrobial assay, and phytochemical screening. The  aqueous, ethanol and  ethyl acetate  extracts  of  the  leaves,  bark  and  roots  of  Distemonantus bentaminaitus , Anogeissus leiocarpus, Garcina kola, Piper guineense and Zanthoxylun Lessmamul for the po...

Evaluation of antimicrobial activities of crude ethyl

The purpose of this research work is to determine the antimicrobial activities of crude ethyl acetate and ethanolic extracts of Morinda lucida leaf, stem, and bark growing in Adekunle Ajasin University Botanical Garden against selected clinical isolates. The plant Morindal lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in color, flowers are w...

Antibacterial and phytochemical properties of five chewing sticks

The antimicrobial activities of the ethanolic, Aqueous and Ethyl acetate extracts of five Nigerian medicinal plant used as chewing sticks were tested against five microorganism, three bacteria and two fungi. The five medicinal plant tested are Vitellaria paradoxa, Vitex doniana, Sarcorcephalus latiolia, Vernonia amygdalina, Olax subcorpidica and the microorganisns are Staphylococcus aureus, Neisseria sieca, Streptococcus mutans and Lactobacillus salivarius and Actinomycetes viscosus. Out of t...

The Study of Antimicrobial Activity of Partially Purified Ethyl acetate Extracts of Bridelia ferruginea on Clinical Isolates

Bridelia ferruginea is commonly grown in Western Tropical Africa. The purpose of this research work is to determine the antimicrobial activities, qualitative and quantitative phytochemical screening, elemental composition, proximate analysis and anti-nutrient composition of purified fraction of Bridelia ferruginea extracts. The organisms used for this research are Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 35659, Pseudomonas aeruginosaATCC 25619,a...

PREVALENCE OF UROPATHOGENIC ESCHERICHIA COLI & STAPHYLOCOCCUS AUREUS AMONG PATIENTS WITH CASES OF URINARY TRACT INFECTIONS ATTENDING BENUE STATE UNIVERSITY TEACHING HOSPITAL, MAKURDI

ABSTRACT In the present study, we investigated the prevalence of few bacterial organisms associated with urinary tracts infection and their antimicrobial susceptibility patterns. Total of Ninety (90) midstream urine samples were collected from outpatients with respect to their age group, gender and marital status attending health facility in Benue State University Teaching Hospital Makurdi. Each 0.1ml of urine sample was cultured on MacConkey agar and colonies showing lactose fermentation we...

Production of protease enzyme from fish guts using Pseudomonas fluorescens, Enterobacter cloacae and Bacillus megaterium.

Food industry wastes are an essential environmental contamination source. A large number of microorganisms including bacteria, yeast and fungi produce different types of enzymes like protease, lipase, chitinase, chitosan and ligninolytic enzyme having high biotechnological interest such as food processing, detergent, textile, pharmaceutical products, and medical therapy. Microbial synthesis of enzymes has been reported to be influenced by various factors such as carbon sources, nitrogen sourc...

Incidence of Staphylococcus aureus in Street Vended Food Sold in Calabar Municipality Nigeria

Street vended foods are the major source of food borne diseases in most parts of sub-Saharan Africa including Nigeria. In this study, six samples each of six different vended foods obtained in Calabar municipality were evaluated for bacteriological quality and presence of Staphylococcus aureususing standard microbiological techniques. Each food was examined macroscopically for evidence of spoilage, followed by culture isolation for Staphylococcus aureus. The organisms isolated were subjected ...

Multidrug Resistant Salmonella enterica Strains in South Western Nigeria: Prevalence and Susceptibility to Ceftriaxone

Aims: To investigate the prevalence and susceptibility pattern Salmonella enterica strains to ceftriaxone in southwestern Nigeria. Place and Duration of Study: Faculty of Pharmacy, University of Ibadan, Nigeria from November 2012-May 2013. Methodology: Isolates of Salmonella enterica were characterized by established standard cultural and biochemical tests and was screened in-vitro for their sensitivity to different antibiotics (ampicillin, amoxicillin, chloramphenicol, cotrimoxazole and ceft...

Microbiology of Meat Spoilage

This paper is titled 'Microbiology of Meat Spoilage'. It clearly explains those Spoilage microorganisms associated with the Spoilage of meat and meat products and also the preservative method to be used in preserving meat. On the basis of its high moisture content and presence of adequate nutrient, meat is regarded   as   the   most   perishable   product   (Magnus,   1986)   and   thereby   highly susceptible   to   spoilage   by   microbial proliferation.  Apart...

Effect of processing on micronutrients (calcium, iron, vitamin A, and zinc) in maize, bambara groundnut, cowpea, cattle bone, roselle calyces, palm oil, Brachystegia eurycoma and Althernanthe

Healthy mature seeds of bambara groundnut (Voandzeia subterranea Thouars), cowpea (Vigna unguiculata), cattle bone, roselle calyces (Hibiscus sabdariffa), red palm oil, yellow maize (Zea mays), and “achi†(Brachystegia eurycoma), were purchased from Ogige market, Nsukka, Nigeria. Methods The processing methods was based on the methods of Uvere et al., (2010). Production of maize-bambara and maize-cowpea food blends  The maize, bambara groundnut and cowpea seeds were cleaned by win...

Microbial Fuel Cell

Introduction Microbial fuel cells (MFCs) are bioreactors that convert chemical energy stored in the bonds of organic matters into electricity through biocatalysis of microorganisms (Potter, 1911; Cohen, 1931; Davis and Yarbrough, 1962; Moon et al., 2006). The schematic of a typical MFC is shown in Fig. 1. In the sketch below, the anodic and cathodic chamber are separated by a proton exchange membrane (PEM) (Wilkinson, 2000; Gil et al., 2003) that allows transport protons while blocking oxygen...

Norovirus

NOROVIRUSNorovirus (NoV), causing the winter vomiting disease, is today recognized as the most important agent of acute human gastroenteritisThe etiology of diarrheas can involve several agents, such as viruses, bacteria, and parasites. Bacterial agents are relatively more important in developing countries, whereas viral agents are more relevant in industrialized countries. The importance of these agents is related to hygiene and sanitation conditions for the population1. In 1972, a 27-nm vir...

Investigation on plastic degrading bacteria

                         Abstract This review lays emphasis on the research works carried out across the globe on plastic degrading bacteria. The field of Microbiology is proving to have immense importance in eradicating pollutants from the environment in the long run. One of the most worked on research is on bacterial species that can degrade biodegradable plastic. Biodegradable plastics are made of monomers which bond via ester or ether bonds. Plastics are considered to be one ...


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