CHARACTERISTIC ph oF HAEMOGLOBINS

ABSTRACT

The thermodynamics of the azide binding reactions of the hybrids of human and canine hemoglobin as well as the isolated a and ß polypeptide chains of human and canine hemoglobin have been studied at various pH's and temperatures. Plots of -AH° against pH, for all the species, show a distinct maximum. The pH at which the maximum value of -M° occurs is termed the 'characteristic pH' (pH

From the results, it is concluded that the value of pH , for the hemoglobin tetramer is an average of the pH^'s of the separate  Oi and ß polypeptide chains although the form of the curve of ~AH° against pH is a function of the tetramer and cannot be obtained by averaging -AH° values for the individual chains.

The Bohr effect of the two hybrids has also been measured and compared with that of the parent hemoglobin.

A study of the equilibrium reaction between oxyhemoglobin and carbon monoxide was carried out and the accompanying thermodynamic data assessed in terms of the mechanisms postulated for explaining ligand binding reactions of methemoglobin.