Research Papers/Topics in Medical Lab Science And Tech
Detection of Bcr-Abl 1 Fusion Gene in Saliva of Chronic Myeloid Leukemic Patients
ABSTRACT The fusion between the “breakpoint cluster region” gene (BCR) and Abelson gene (ABL1) is the molecular hallmark of most cases of chronic myeloid leukaemia and some cases of acute lymphoblastic leukaemia. The objective of this study was to detect BCR-ABL1 in saliva of known CML patients collected in Oragene-RNA kit (DNA Genotek, Canada) and to see if mRNA could be preserved for a longer period in the saliva samples than in blood samples. A comparison of the BCR-ABL ratios in...
Coagulation Profile of Non-Diabetic Hypertensive Patients in Enugu Urban Enugu State
Abstract Fifty (50) Non-diabetic Hypertensive Patients in the medical out-patient (MOP) department of University of Nigeria Teaching Hospital (UNTH), Ituku Ozalla, Enugu, Nigeria aged 40-78 years comprising 25 males and 25 females were investigated. Also fifty age and sex-matched apparently healthy subjects served as the control group. The following variables were determined using standard methods: prothrombin Time, Activated partial Thromboplastin Time, Fibrinogen Assay, platelet Coun...
ALTERNATIVE STAINING OF KIDNEY TISSUE SECTIONS WITH EXTRACTS OF TUMERIC (Curcuma Longa) AND ZOBO (Hibiscus Sabdariffa)
AbstractMost stains used in histopathology are synthetic, toxic and costly for use. Hence, this study was carried out at the Histopathology Laboratory of Imo State University to find out the probability of replacing routine toxic and costly stains for nature, non-toxic, less costly and effective stains. Sixteen (16) kidney tissue sections were used for this study obtained from the Federal Medical Center, Owerri of which was divided into two groups of eight (8) kidney sections respectively. Th...
Prevalence of Pseudomonas Aeruginosa in Hospitalised Patients in Kubwa General Hospital, Abuja, FCT
ABSTRACT The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10) each of urine, skin lesions and pus. And also three environmental samples (tap water, sand and flower) were collected. The samples obtai...
Extraction and Thermal Degradation of Caffeine Extracted From Cola Nut
ABSTRACT Caffeine is an odorless, slightly bitter organic compound found mostly in beverages such as coffee or tea and in chocolate. It is the most widely used “mind-altering drug” in the world, though it is considered safe and is mostly unregulated. Caffeine acts as a mild stimulant on the nervous system, aiding in alertness. Caffeine is also included in many weight loss supplements due to its reputation of acting as an appetite suppressant and in sti...
DETERMINATION AND COMPARISON OF APPARENT CUBIC EXPANSITIVITY OF GLYCERINE AND HONEY
This project work investigated the apparent cubic expansitivity of glycerine and honey and they were compared. The mass of glycerine and honey before heating were recorded as 75g both respectively, while after heating they were reduced to 65.50g and 71.00g respectively. The glycerine and honey were both heated on a Bunsen burner. The initial and final tempreture of the liquids were recorded (for glycerine and honey) was 31.00ºC and 99.00ºC for glycerine and 31.00ºC and 100ºC for hon...
THE EFFECT OF TEMPERATURE AND COOKING TIME ON THE PROTEIN CONTENT OF THREE SPECIES OF COWPEA
ABSTRACT The study titled "The effect of temperature and cooking time on the protein content of three species of cowpea" is aimed at determining and comparing temperature effect on the protein content of cowpea. Three different species of cowpea was obtained at tudun wada market. The cowpea sample was cooked at different temperature and cooking time. The cooked sample was oven dried and the protein level of the three sample was determined. The result showed that Vigna textilis (Red...
PROJECT ON CONSTRUCTION AND TESTING OF A LIGHT DIMMER SWITCH
This project (light Dimmer Switch) is base on adjusting the voltage which gets to the lamp. The project use an alternating current as an input at the rate 240v from the socket outlet. The circuit is mainly used to control the desired brightness of the lamp of 100watts, by limiting the flow of current through the lamp by their equivalent resistance, which the capacitor used to store same charges when the variable resistor is varied. The project dimmer switch can be used in so many place...
DESIGN AND CONSTRUCTION OF ALCOHOL DETECTOR
ABSTRACT An alcohol detector: - is a electronic device that detect alcohol in the breath of a person that has consumed the liquire. In this project, the design involved the hardware module and software module. The hardware module consist of sensor circuit (MQ – 3), the pic (Microcontroller circuit), the L.C.D. Circuit and the alarm circuit. The software module include, the programming on plc microcontroller that sends the information to the encoder using C – programming, the ...
The cost of treatment of sickle cell anaemia
ABSTRACTHealth care is a necessity and a basic human need. Households or individuals often derived various strategies to cope with large health expenditure. Sickle-cell Anaemia is the most frequent haemoglobin disorder and one of the world’s greatest cause of morbidity and mortality mostly in sub-Saharan Africa. This research work title the cost of treatment of sickle cell anaemia (A case study of General Hospital Katsina) was carried out in General Hospital Katsina, Katsina state Nigeria ...
ANTIBACTERIAL ACTIVITY OF HONEY ON Streptococcus pyogenes, Escherichia coli AND Staphylococcus aureus ISOLATED FROM BURN WOUNDS OF PATIENTS ATTENDING ST. MARY’S HOSPITAL LACOR
The growing threat and spread of antibiotic resistance by a wide range of common pathogens has led to increased investigations into traditional medicinal plants as alternatives. Honey has been used as a medicine since ancient times in many cultures and is still used in ‘folk medicine’. The moist environment of chronic wounds is an ideal growth medium for bacteria thereby causing delay in healing. Honey is commonly used as a topical antibacterial agent in most cases to complement th...
PREVALENCE OF Plasmodium AND Salmonella INFECTIONS AMONG PREGNANT WOMEN IN ABA, ABIA STATE
ABSTRACTMalaria and typhoid fever are both endemic in the tropics and pregnant women constitute one of the high risk groups. The study on prevalence of Plasmodium and Salmonella infections among pregnant women in Aba, Abia State was carried out in Aba between June and July, 2016. Blood samples from 200 volunteered pregnant women who came for antenatal clinic with clinical signs in three selected health institution were collected and analyzed at microbiology laboratory, Abia State Polytechnic ...
Weight changes and histomorphological effects of marijuana on the brain of adult wistar rats
ABSTRACT The present study was conducted in Ekpoma, headquarters of Esan West Local Government area of Edo state. The aim of the study was to determine the effect of marijuana on the heart of wistar rats. The study involved twenty four growing wistar rats having weights between 250g-275g divided into 4 groups; control and groups 1-3, comprising of 6 rats each. The control group received only grower’s mash and distilled water while groups 1, 2 and 3 received 0.02mg/kg body weight...
Hepatoprotective Potential of Russelia Equisetiformis Plant Extract on Drug-Induced Hepatotoxicity in Experimental Models
Background: A large number of herbal medicines are widely in-use to offer relief from hepatic injury,although attempts are still ongoing globally to get scientific proofs for these orthodox remedies, there isstill paucity of data on Russelia equisetiformes. There are solitary reports of anti-inflammatory, analgesic,and antinociceptive properties. Meanwhile, inflammation is a hallmark of hepatic injury. Further study maypave way for next generation hepatoprotective medication.Methods: The expe...
DISTRIBUTION OF ABO, Rh (RHESUS) BLOOD GROUPING AND HEPATITIS B AMONG BLOOD DONORS WITH NATIONAL BLOOD TRANSFUSION SERVICE KADUNA
ABSTRACTABO and Rh blood group systems are of major clinical significance amongst the thirty (30) human blood group system. The Distribution of ABO, Rh blood grouping and hepatitis B virus among blood donors with National Blood Transfusion Service, Kaduna was carried out. Two hundred voluntary blood donors (200) were used. Questionnaire was used to obtain the respondents’ bio-demographic data. Microplate technique was used for ABO and Rh blood grouping tests and ELISA kit (Monolisa HBsAg UL...