Research Papers/Topics in Medical Lab Science and Tech

Phytochemical Screening and Determination of Trace Element in Gliricidia Leaf

ABSTRACTS:This paper discuss the elemental composition and concentration of Gliricidia sepium leaves by energy dispersive x-ray fluorescence.  The element present are Cl, K, Ca, Mn, Fe, Ni, Cu, Zn. Also discuss on  Phytochemicals of the leaves wish contain alkaloids, tannins ,and saponins. The used analytical method, the experiment setup and the procedure of the sample preparation are presented.  TABLE OF CONTENTTitle pageCertificationDedicationAcknowledgment...

Immobilized Enzymes for Industrial Applications

TABLE OF CONTENTS CHAPTER ONE 1.0      Introduction 1.1      Enzymes 1.2      Classification of Enzymes 1.3      Factors affecting enzymes activities 1.4      Kinetic of Enzymes catalyzed reactions CHAPTER TWO 2.0      Immobilization process 2.1    Immobilized Enzymes 2.2 &nbs...

Anti-Plasmodial Property of Moringa Oleifera Seed Extract on Swiss Mice

 TABLE OF CONTENTS Contents                                                                             &nb...

Molecular Detection Of Mycobacterium Bovis In Cattle Milk And Abattoir Staff In Enugu

ABSTRACT The study was carried out to detect Mycobacterium bovis in cattle milk and abattoir staff in Enugu. Blood samples were collected from 50 subjects at Artisan and Ogbete market abattoirs with 25 samples from each location. Fifty Milk samples were collected from cattle at Fulani settlements which comprised of 13 from Gariki, 16 from 9th Mile and 21 from Monarch in Enugu. Milk samples were cultured on Lowenstein Jensen medium and incubated for 8weeks at 37o C. DNA was extracted fr...

Physico-Chemical And Bacteriological Properties Of Water Supply Sited Around 9th Mile Brewery, Enugu State

ABSTRACT Analysis of the physico-chemical and bacteriological properties of Ajalli stream, Ngwo Spring water, Umuagba Ime-Owa River, Abonuzu stream and Ibute Amaeke hand dug well water all around 9th mile brewery in Enugu State were investigated between June 2009 and May 2010 to assess the extent of pollution of the water due to effluent discharges from the brewery industries around the studied area. These sites were investigated during the dry season and rainy season of the research p...

The Occurrence Of Hepatitis C Virus Infection In Apparently Healthy Subjects In Enugu Metropolis, Nigeria

ABSTRACT It is widely known that hepatitis C virus (HCV) slowly and progressively damages the liver while the human host may be unaware of the infection. The aim of this study was to investigate the prevalence of HCV in apparently healthy individuals in Enugu metropolis. This is important because it will explain the prevailing liver disease experienced in the society. A total of 214 apparently healthy individuals were recruited from Enugu North, Enugu South and Enugu East for this study...

Studies On The Extra-Human Distribution Of Enterohaemorrhagic Escherichia Coli From Borno And Enugu States Of Nigeria

ABSTRACT Enterohaemorrhagic Escherichia coli (EHEC) a causative agent of haemolytic uremic Syndrome (HUS) and haemorrhagic colitis especially in children, believed to originate from cattle is increasing with the day in parts of Nigeria. In order to ascertain the probable sources to assist a control regimen, laboratory analysis was carried out using standard bacteriological techniques for the isolation and identification of Escherichia coli (E.coli) and EHEC.A study was done on the distr...

Detection of Bcr-Abl 1 Fusion Gene in Saliva of Chronic Myeloid Leukemic Patients

ABSTRACT The fusion between the “breakpoint cluster region” gene (BCR) and Abelson gene (ABL1) is the molecular hallmark of most cases of chronic myeloid leukaemia and some cases of acute lymphoblastic leukaemia. The objective of this study was to detect BCR-ABL1 in saliva of known CML patients collected in Oragene-RNA kit (DNA Genotek, Canada) and to see if mRNA could be preserved for a longer period in the saliva samples than in blood samples. A comparison of the BCR-ABL ratios in...

Coagulation Profile of Non-Diabetic Hypertensive Patients in Enugu Urban Enugu State

Abstract Fifty (50) Non-diabetic Hypertensive Patients in the medical out-patient (MOP) department of University of Nigeria Teaching Hospital (UNTH), Ituku Ozalla, Enugu, Nigeria aged 40-78 years comprising 25 males and 25 females were investigated. Also fifty age and sex-matched apparently healthy subjects served as the control group. The following variables were determined using standard methods: prothrombin Time, Activated partial Thromboplastin Time, Fibrinogen Assay, platelet Coun...

ALTERNATIVE STAINING OF KIDNEY TISSUE SECTIONS WITH EXTRACTS OF TUMERIC (Curcuma Longa) AND ZOBO (Hibiscus Sabdariffa)

AbstractMost stains used in histopathology are synthetic, toxic and costly for use. Hence, this study was carried out at the Histopathology Laboratory of Imo State University to find out the probability of replacing routine toxic and costly stains for nature, non-toxic, less costly and effective stains. Sixteen (16) kidney tissue sections were used for this study obtained from the Federal Medical Center, Owerri of which was divided into two groups of eight (8) kidney sections respectively. Th...

Prevalence of Pseudomonas Aeruginosa in Hospitalised Patients in Kubwa General Hospital, Abuja, FCT

                                      ABSTRACT The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10) each of urine, skin lesions and pus. And also three environmental samples (tap water, sand and flower) were collected. The samples obtai...

Extraction and Thermal Degradation of Caffeine Extracted From Cola Nut

ABSTRACT     Caffeine is an odorless, slightly bitter organic compound found mostly in beverages such as coffee or tea and in chocolate.  It is the most widely used “mind-altering drug” in the world, though it is considered safe and is mostly unregulated.  Caffeine acts as a mild stimulant on the nervous system, aiding in alertness.  Caffeine is also included in many weight loss supplements due to its reputation of acting as an appetite suppressant and in sti...

DESIGN AND CONSTRUCTION OF ALCOHOL DETECTOR

ABSTRACT An alcohol detector: - is a electronic device that detect alcohol in the breath of a person that has consumed the liquire. In this project, the design involved the hardware module and software module. The hardware module consist of sensor circuit (MQ – 3), the pic (Microcontroller circuit), the L.C.D. Circuit and the alarm circuit. The software module include, the programming on plc microcontroller that sends the information to the encoder using C – programming, the sensor detec...

The cost of treatment of sickle cell anaemia

ABSTRACTHealth care is a necessity and a basic human need. Households or individuals often derived various strategies to cope with large health expenditure. Sickle-cell Anaemia is the most frequent haemoglobin disorder and one of the world’s greatest cause of morbidity and mortality mostly in sub-Saharan Africa. This research work title the cost of treatment of sickle cell anaemia (A case study of General Hospital Katsina) was carried out in General Hospital Katsina, Katsina state Nigeria ...

ANTIBACTERIAL ACTIVITY OF HONEY ON Streptococcus pyogenes, Escherichia coli AND Staphylococcus aureus ISOLATED FROM BURN WOUNDS OF PATIENTS ATTENDING ST. MARY’S HOSPITAL LACOR

The growing threat and spread of antibiotic resistance by a wide range of common pathogens has led to increased investigations into traditional medicinal plants as alternatives. Honey has been used as a medicine since ancient times in many cultures and is still used in ‘folk medicine’. The moist environment of chronic wounds is an ideal growth medium for bacteria thereby causing delay in healing. Honey is commonly used as a topical antibacterial agent in most cases to complement th...


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