Research Papers/Topics in Medical Lab Science and Tech

The Effect of Ocimum Tenuiflorum (Nchuanwu) Leaf Extract on Hematolgical Parameters of Immumnosuppressed Albino Rats.

ABSTRACT The effect of Ocimum tenuiflorum on hematological parameters in immunosuppressed albino rats was investigated in this study. The aqueous (AE) and methanol extract (ME) of the leaf were obtained. Acute toxicity study was done to determine the LD50 of the leaf extract. Rats of mixed sexes, aged 2- 3months, weighing 150 to 240 grams were used. The rats were divided into 8 groups A-H of four rats each. Group A served as normal control. Immunesuppression was induced using 3mg/kg bodyweig...

Assessment of Biochemical Markers of Iron and Some Haematological Parameters on Blood Donors in Enugu State

ABSTRACT Iron deficiency is one of the most common of the nutritional deficiencies. The eventual consequence of iron deficiency is iron deficiency anemia; where the body stores of iron have been depleted and the body is unable to maintain level of hemoglobin in the blood. Blood donation leads to a substantial iron loss, as about 0.5mg iron is lost per each milliliter of blood donated. It is evident that haemoglobin level alone is inadequate for detecting blood donors with iron deficiency with...

Effects of Oral Administration of Leaf Extract of Uvaria Chamae (Mmimi Ohia) in Albino Wistar Rats

ABSTRACT Uvaria chamae P. Beauv is known to have various medicinal and therapeutic properties. The Biochemical and toxicological effects of the leaf extract were assessed in this study using Albino wistar rats. Acute toxicity test was performed on the rats to determine the LD50.Sub acute toxicity test was also carried out by oral administration of different doses of ethanolic extract of U. chamae leaf (EEUC) on different groups of rats for thirty (30) days. Twenty (20) male albino wistar rats...

Measurement of Prothrombin Time and Activated Partial Thromboplastin Time in Sudanese Sickle Cell Anemia Patients in Khartoum State

Abstract This is a case control study aimed to measure levels of, Prothombin time (PT), activated partial Thromboplastin time (APTT), platelet count in patients with homozygous sickle cell disease (HbSS), sickle cell trait (HbAS) and normal controls (HbAA). The study period from August to October 2019, in Khartoum State in the Sickle Cell Clinic of Gaffer Ibn Auf Children Specialized Hospital and Al-ribat University hospital. The study population comprises two groups of children in different ...

Phytochemical Screening and Determination of Trace Element in Gliricidia Leaf

ABSTRACTS: This paper discuss the elemental composition and concentration of Gliricidia sepium leaves by energy dispersive x-ray fluorescence.  The element present are Cl, K, Ca, Mn, Fe, Ni, Cu, Zn. Also discuss on  Phytochemicals of the leaves wish contain alkaloids, tannins ,and saponins. The used analytical method, the experiment setup and the procedure of the sample preparation are presented.   TABLE OF CONTENT Title page Certification Dedication Acknowledgment Abstract Table of...

Immobilized Enzymes for Industrial Applications

TABLE OF CONTENTS CHAPTER ONE 1.0      Introduction 1.1      Enzymes 1.2      Classification of Enzymes 1.3      Factors affecting enzymes activities 1.4      Kinetic of Enzymes catalyzed reactions CHAPTER TWO 2.0      Immobilization process 2.1    Immobilized Enzymes 2.2    Immobilization techniques and support materials 2.3    Inhibition of Enzymes   2.4    Enzymes Mechanisms CHAPTER THREE 3.0      Industrial Applications of Immobilized...

Molecular Detection Of Mycobacterium Bovis In Cattle Milk And Abattoir Staff In Enugu

ABSTRACT The study was carried out to detect Mycobacterium bovis in cattle milk and abattoir staff in Enugu. Blood samples were collected from 50 subjects at Artisan and Ogbete market abattoirs with 25 samples from each location. Fifty Milk samples were collected from cattle at Fulani settlements which comprised of 13 from Gariki, 16 from 9th Mile and 21 from Monarch in Enugu. Milk samples were cultured on Lowenstein Jensen medium and incubated for 8weeks at 37o C. DNA was extracted from bot...

Physico-Chemical And Bacteriological Properties Of Water Supply Sited Around 9th Mile Brewery, Enugu State

ABSTRACT Analysis of the physico-chemical and bacteriological properties of Ajalli stream, Ngwo Spring water, Umuagba Ime-Owa River, Abonuzu stream and Ibute Amaeke hand dug well water all around 9th mile brewery in Enugu State were investigated between June 2009 and May 2010 to assess the extent of pollution of the water due to effluent discharges from the brewery industries around the studied area. These sites were investigated during the dry season and rainy season of the research period....

The Occurrence Of Hepatitis C Virus Infection In Apparently Healthy Subjects In Enugu Metropolis, Nigeria

ABSTRACT It is widely known that hepatitis C virus (HCV) slowly and progressively damages the liver while the human host may be unaware of the infection. The aim of this study was to investigate the prevalence of HCV in apparently healthy individuals in Enugu metropolis. This is important because it will explain the prevailing liver disease experienced in the society. A total of 214 apparently healthy individuals were recruited from Enugu North, Enugu South and Enugu East for this study. The...

Studies On The Extra-Human Distribution Of Enterohaemorrhagic Escherichia Coli From Borno And Enugu States Of Nigeria

ABSTRACT Enterohaemorrhagic Escherichia coli (EHEC) a causative agent of haemolytic uremic Syndrome (HUS) and haemorrhagic colitis especially in children, believed to originate from cattle is increasing with the day in parts of Nigeria. In order to ascertain the probable sources to assist a control regimen, laboratory analysis was carried out using standard bacteriological techniques for the isolation and identification of Escherichia coli (E.coli) and EHEC.A study was done on the distributi...

Detection of Bcr-Abl 1 Fusion Gene in Saliva of Chronic Myeloid Leukemic Patients

ABSTRACT The fusion between the “breakpoint cluster region” gene (BCR) and Abelson gene (ABL1) is the molecular hallmark of most cases of chronic myeloid leukaemia and some cases of acute lymphoblastic leukaemia. The objective of this study was to detect BCR-ABL1 in saliva of known CML patients collected in Oragene-RNA kit (DNA Genotek, Canada) and to see if mRNA could be preserved for a longer period in the saliva samples than in blood samples. A comparison of the BCR-ABL ratios in bloo...

Coagulation Profile of Non-Diabetic Hypertensive Patients in Enugu Urban Enugu State

Abstract Fifty (50) Non-diabetic Hypertensive Patients in the medical out-patient (MOP) department of University of Nigeria Teaching Hospital (UNTH), Ituku Ozalla, Enugu, Nigeria aged 40-78 years comprising 25 males and 25 females were investigated. Also fifty age and sex-matched apparently healthy subjects served as the control group. The following variables were determined using standard methods: prothrombin Time, Activated partial Thromboplastin Time, Fibrinogen Assay, platelet Count, Fas...

ALTERNATIVE STAINING OF KIDNEY TISSUE SECTIONS WITH EXTRACTS OF TUMERIC (Curcuma Longa) AND ZOBO (Hibiscus Sabdariffa)

Abstract Most stains used in histopathology are synthetic, toxic and costly for use. Hence, this study was carried out at the Histopathology Laboratory of Imo State University to find out the probability of replacing routine toxic and costly stains for nature, non-toxic, less costly and effective stains. Sixteen (16) kidney tissue sections were used for this study obtained from the Federal Medical Center, Owerri of which was divided into two groups of eight (8) kidney sections respectively. T...

Prevalence of Pseudomonas Aeruginosa in Hospitalised Patients in Kubwa General Hospital, Abuja, FCT

                                      ABSTRACT The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10) each of urine, skin lesions and pus. And also three environmental samples (tap water, sand and flower) were collected. The samples obtained were cult...


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