Research Papers/Topics in Applied Microbiology

Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas

Oryza sativa husks and peels of Musa paradisiaca were pretreated by hydrothermal and acid fermentation using cassava mill effluent, and co-digested in a laboratory scale reactor maintained at 45 ± 2 ⁰C for 42 days. Five digesters were used for the experiment and each digester contained equal proportion of Oryza sativa husks and Musa paradisiaca in the ratio of 1:1; and cow dung (the inoculum) was added across. Substrate in Digester X (Control) was not pretreated. Digester A contained Oryza...

Evaluation of the phytochemical composition and antimicrobial activity of lemongrass (Cymbopogon citratus) against some human pathogenic microorganisms

ABSTRACTAntibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus (lemon grass) against selected microbial pathogens using chloroform and methanol as extracting solvents were assessed. The minimum inhibitory, bacteric...

Hepatitis B infection

INTRODUCTION        Hepatitis B is a viral disease process caused by the Hepatitis B virus (HBV). Hepatitis B virus is a major cause of acute and chronic hepatitis worldwide and is more prevalence in developing countries. More than 2 billion people are infected with HBV worldwide while some 280million are chronic carriers, harbouring the virus in their liver. The disease is responsible for 80% of all cases of primary liver cases, which is one of leadin...

Bacteriological analysis of flooded soil in Benin areas, Edo State

ABSTRACT Microorganisms are important components in the structure and function of organic matter in soil, hence this work was done to identify bacterial communities present in flooded soil. Soil samples were collected from eight different areas within Benin metropolis, Edo State, Nigeria. The samples were cultured on using pour plate method, identification of the main bacterial groups was carried out based on cultural, morphology and biochemical test. The results obtained showed the to...

Physicochemicls and microbial quality of local fermented milk

ABSTRACTCulturally, consumption of local fermented skimmed milk (nono/tsala) is common in Northern Nigeria and many other parts of Africa and it is sometimes associated with microbial diseases due to poor hygienic practice. Hence, this study was conducted with the objectives of assessing the microbial quality and some physicochemical parameters, a purposive sampling was used to collect a total of 12 different samples from Dutse local government, Jigawa state. The results obtained showed that ...

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC   A RESEARCH PROJECT   ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in the two set of egg sample  carried out showed the mean total bacterial count as 0.33x102 – 3.6 x 102 ...

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS   A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 107 to 11.5 X108cfu/g for unsealed and 1.5 X107 to 9.2 x 107cfu/g  for sealed. The following bacterial isolates were identified these include Bacillus sp, klebsiellssp, staphylococcus sp Escherichia sp, Lactrbacillussp, proteussp an...

ANTIVIRAL EFFECT OF MANGIFERA INDICA AND THYMUS VULGARIS LEAVES EXTRACT ON NEWCASTLE DISEASE VIRUS

Abstract BackgroundNewcastle disease  is a highly contagious disease of birds caused by Paramyxovirus. Birds mostly affected by Paramyxovirus virus includes; chickens, turkeys, geese, ducks and guinea fowls. Newcastle disease however, remains a worldwide problem. There are three major strains of Newcastle disease virus and they are velogenic, mesogenic and lentogenic strains respectively. These viral strains reflects the severity of the disease pathotypes. In the medical laboratory,  these...

MICROBIAL QUALITY OF READY TO EAT FOODS AND SOME VEGETABLES SOLD AT AFOR-ULE MARKET, ABA.

Bacteria are group of microorganisms all of which lack a distinct nuclear membrane (and hence are considered more primitive than animal and plant cells) and of most which have a cell wall of unique composition. Most bacteria are unicellular; the cells maybe spherical (coccu), rod-shaped (bacillus), spiral (spirillum), comma-shaped (vibrio) or corkscrew-shaped (spierocheate). Generally, they range in size between 0.5 and 5um. (Elizabeth and Martin, 2003). Food is any substance that p...

Phytochemical Screening and Evaluation of Antioxidant and Proximate Properties of Morinda lucida Ethanolic Extract

Morinda lucida is a tropical West African evergreen shrub or small to medium sized rainforest tree, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. This study was designed to carry out the preliminary phytochemical screening and evaluation antioxidant and proximate properties of ethanol extracts prepared from the bark, leaves and root of M. lucida . It revealed the presence of saponin, tannins, a...

Phytochemical screening and antimicrobial properties of partially purified ethyl acetate

Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms used for the study are Escherichia coli, staphylococcus aureus, Candida albicans and Klebsiella pneumonia. The leaf and bark were extracted using one e...

Phytochemical Composition and Antimicrobial Activity of Daniella oliveri Extracts

Daniella oliveri (Caesalpiniacea) is a plant found in the Amazon region and other parts of South America and Africa. The tree may reach a height of 100 feet and trunk diameter of 4 feet. Daniella oliveri (Rolfe) Fabiaceae is commonly known as Ilorin balsam (eepo-iya) or Copaihu africana . It is an indigenous African tree, found extensively in Benin, Cameroon, Gambia and Nigeria. The basic objective of this project is to determine the antimicrobial activity and phytochemical composition of Dan...

Evaluation of Antibacterial Potency of Crude Ethyl Acetate and Ethanol Extracts of Morinda lucida

The plant Morinda lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in colour, flowers are white in colour, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The purpose of this work is to determine the antimicrobial properties of      Morinda lucida against Mycobacterium species, a very virulent and infectious organism, isolated...

Elemental Composition, Evaluation of Anti-nutrients, and Antioxidant Potentials of Morinda lucida

Morinda lucida is a tropical West African tree of medium-size, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. The preliminary phytochemical screening of different extracts prepared from bark, leaves and seeds of M. lucida revealed the presence of Saponin, Tannins, Alkaloids, Flavonoids, and Cardiac glucosides. Furthermore, it also reveals presence of Minerals like Sodium, Potassium, Calcium, Iro...

Evaluation of Antimicrobial Activity, Phytochemical Screening and Gas Chromatography-Mass/ Spectrophotometric Profile of Essential Oil from Persea americana & Citrus sinensis

AbstractThis study aims to determine the phytochemical characteristics, structural determination of bioactive component from the leaf extract  using GCMs and experimentally investigation of the antibacterial activity of essential oil from Persea americana and Citrus sinensis leaf against seven bacteria (Gram-positive and Gram-negative) which are Staphylococcus aureus , Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia, Escherichia coli, Proteus mirabilis and Pseudomonasaeruginosa...

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