Applied Microbiology Research Papers/Topics

Studies On Microbiological And Physico- Chemical Analyses Of Commercially And Laboratory Prepared Orange Juices

ABSTRACT Microbiological and physico-chemical analyses and sensory evaluation were carried out on commercially and freshly prepared orange juice (100%) in the laboratory and its shelf- life after the storage period of 90 days using various storage methods. At the end of the study, the result showed that the laboratory processed orange juice in terms of the nutritional composition when compared with the commercially processed orange juice had a better quality considering the parameters assess...

Microfluidics for Drug Screening and Paralysis of Parasites

The emergence of new drugs is often driven by the escalating resistance of parasites to existing drugs and the accessibility of more advanced technology platforms. Microfluidic devices have allowed for quicker testing of compounds, regulated sampling/sorting of entire animals, and automated behavioral pattern detection. In the majority of microfluidic devices, the effects of drugs on small animals (e.g. Caenorhabditis elegans)elegant are quantified by an endpoint, dose response curve that sho...

Extraction of DNA in Tomatoes and Garden Eggs Fruits

ABSTRACT This project is basically concerned with the extraction of DNA in tomatoes and garden egg fruit. An experiment on how to extract DNA on tomatoes and garden egg, isolation on it to see what DNA actually look like as well as importance of DNA extraction are consid3ered in this project. TABLE OF CONTENT Title page ……………………………………………………… i. Certification……………………………………………………..ii Dedication………………...

Prevalence of Dermatophytosis Among Primary School Children at Wuntin Dada Primary School Bauchi

DERMATOPHYTE are unique of fungi that effect the Keratinous tissues of lower animals and human Dermatophytoses is the medical term for fungal infection of the superficial layer of the skin, nail, hair and scalp.  The prevalence study Dermatophytoses in Wuntin Dada Primary School Bauchi is to determine the prevalence of the diseases, the different clinical types and actual species responsible for the infections.  Out of 950 pupils examined, 105 children were found to be with suspected Dermat...

Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas

Oryza sativa husks and peels of Musa paradisiaca were pretreated by hydrothermal and acid fermentation using cassava mill effluent, and co-digested in a laboratory scale reactor maintained at 45 ± 2 ⁰C for 42 days. Five digesters were used for the experiment and each digester contained equal proportion of Oryza sativa husks and Musa paradisiaca in the ratio of 1:1; and cow dung (the inoculum) was added across. Substrate in Digester X (Control) was not pretreated. Digester A contained Oryza...

Evaluation of the phytochemical composition and antimicrobial activity of lemongrass (Cymbopogon citratus) against some human pathogenic microorganisms

ABSTRACT Antibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus (lemon grass) against selected microbial pathogens using chloroform and methanol as extracting solvents were assessed. The minimum inhibitory, bacteri...

Hepatitis B infection

INTRODUCTION         Hepatitis B is a viral disease process caused by the Hepatitis B virus (HBV). Hepatitis B virus is a major cause of acute and chronic hepatitis worldwide and is more prevalence in developing countries. More than 2 billion people are infected with HBV worldwide while some 280million are chronic carriers, harbouring the virus in their liver. The disease is responsible for 80% of all cases of primary liver cases, which is one of leading causes of death in Asia and Af...

Bacteriological analysis of flooded soil in Benin areas, Edo State

ABSTRACT  Microorganisms are important components in the structure and function of  organic matter in soil, hence this work was done to identify bacterial communities  present in flooded soil. Soil samples were collected from eight different areas  within Benin metropolis, Edo State, Nigeria. The samples were cultured on using  pour plate method, identification of the main bacterial groups was carried out  based on cultural, morphology and biochemical test. The results obtained showed ...

Physicochemicls and microbial quality of local fermented milk

ABSTRACT Culturally, consumption of local fermented skimmed milk (nono/tsala) is common in Northern Nigeria and many other parts of Africa and it is sometimes associated with microbial diseases due to poor hygienic practice. Hence, this study was conducted with the objectives of assessing the microbial quality and some physicochemical parameters, a purposive sampling was used to collect a total of 12 different samples from Dutse local government, Jigawa state. The results obtained showed that...

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC   A RESEARCH PROJECT   ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in the two set of egg sample  carried out showed the mean total bacterial count as 0.33x102 – 3.6 x 102 cfu/ml. While the antibiot...

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS   A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 107 to 11.5 X108cfu/g for unsealed and 1.5 X107 to 9.2 x 107cfu/g  for sealed. The following bacterial isolates were identified these include Bacillus sp, klebsiellssp, staphylococcus sp Escherichia sp, Lactrbacillussp, proteussp and Citrobacter...

ANTIVIRAL EFFECT OF MANGIFERA INDICA AND THYMUS VULGARIS LEAVES EXTRACT ON NEWCASTLE DISEASE VIRUS

Abstract  Background Newcastle disease  is a highly contagious disease of birds caused by Paramyxovirus. Birds mostly affected by Paramyxovirus virus includes; chickens, turkeys, geese, ducks and guinea fowls. Newcastle disease however, remains a worldwide problem. There are three major strains of Newcastle disease virus and they are velogenic, mesogenic and lentogenic strains respectively. These viral strains reflects the severity of the disease pathotypes. In the medical laboratory,  the...

MICROBIAL QUALITY OF READY TO EAT FOODS AND SOME VEGETABLES SOLD AT AFOR-ULE MARKET, ABA.

Bacteria are group of microorganisms all of which lack a distinct nuclear membrane (and hence are considered more primitive than animal and plant cells) and of most which have a cell wall of unique composition. Most bacteria are unicellular; the cells maybe spherical (coccu), rod-shaped (bacillus), spiral (spirillum), comma-shaped (vibrio) or corkscrew-shaped (spierocheate). Generally, they range in size between 0.5 and 5um. (Elizabeth and Martin, 2003). Food is any substance that people or ...

Phytochemical Screening and Evaluation of Antioxidant and Proximate Properties of Morinda lucida Ethanolic Extract

Morinda lucida is a tropical West African evergreen shrub or small to medium sized rainforest tree, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. This study was designed to carry out the preliminary phytochemical screening and evaluation antioxidant and proximate properties of ethanol extracts prepared from the bark, leaves and root of M. lucida . It revealed the presence of saponin, tannins, a...

Phytochemical screening and antimicrobial properties of partially purified ethyl acetate

Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms used for the study are Escherichia coli, staphylococcus aureus, Candida albicans and Klebsiella pneumonia. The leaf and bark were extracted using one e...


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