Research Papers/Topics in Biochemistry

Hybridoma Technology

  INTRODUCTION: Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies ). This process starts by injecting a mouse (or other mammal) with an antigen that provokes an immune response. A type of white blood cell, the B cell, produces antibodies that bind to the injected antigen. These antibody producing B-cells are then harvested from the mouse and, in turn, fused with immortal B cell cancer cells, a myeloma ,  to prod...

Garlic Bulb Prevents High Blood Pressure in Egg Yolk induced Hypertensive Rats

The aim of this study is to access the antihypertensivepotential of garlic juice in rats. To achieve this, 30 Wistar ratswere divided into three groups of 10 each. Rats in groups 1 and 2received aspecial prepared egg feed diet (24 egg yolkmixed with 1kg of normal rat diet) while those in group 3 received normalfeed diet and they served as the control group. Animals in group2 were administered 3 mL/100g body weight of undiluted garlicjuice simultaneously with the feeding. The animals were fedo...

Egg Yolk induces High Blood Pressure in Wistar Rats

Hypertension constitutes a major risk factor forseveral cardiovascular diseases such asatherosclerosis, heart failure, renal insufficiency,coronary artery disease and stroke. The risk factorincreases with age in both sexes. According to WHO,about one-third of the world’s population suffersfrom hypertension and the incidence has beenincreasing at a rapid rate due to life stylemodification. This study is aimed at investigating theeffect of egg yolk on blood pressure of Wistar rats. Toachieve ...

Effect of Consumption of Garri Processed by Traditional and Instant Mechanical Methods on Lipid Profile of Wistar Rats

Aim: This study focuses on the effect of consumption of garri processed by traditional and instantmechanical methods on the lipid profile of Wistar rats.Methods: Cassava samples were obtained from the International Institute of Tropical Agriculture(IITA), Ibadan and were processed using traditional and instant mechanical methods. Fifteen adultmale Wistar rats were purchased from the Animal Holding Unit of the Department of Physiology,University of Ibadan, Nigeria with body weight between 100 ...

Air Freshener induced Oxidative Stress and Adveresly affects Immunity

Recently, the use of ‘sunlight’ air freshener in Nigeria is on the increase. Some people use airfreshener in the office, car as well as in their homes, thereby constantly being exposed to it withoutconsideration of its adverse effect on human health. This study is therefore aimed at assessing theeffect of “sunlight” air freshener on oxidative stress biomarkers. Thirty (30) Wistar rats were dividedinto three groups of ten each and kept in different rooms. Rats in group 1 were not expos...

Effect of Common Household Insecticides Used in Nigeria on Rat Male Reproductive Hormones

Background: The use of household insecticides for the eradication of insects especially mosquitoesin Nigeria is increasing. These insecticides are used without consideration of their adverse effect onhuman health.Aim: This study was aimed at accessing the effect of common household insecticides used inNigeria on male reproductive hormones of Wistar rats.Methodology: Thirty male Wistar rats were divided into five groups of six each and kept in differentrooms. Rats in group 1 were exposed to Ra...

Consumption of Coconut (CocosnuciferaL.) WaterImproved Fertility Parameters in Male Wistar Rats

Background: The need for fertility stimulation in men and women cannot be overemphasizedespecially when a marriage is childless in some communities in Nigeria. While chemical methodsfor fertility stimulation may have some side effects and are not readily available, remedy fromnatural sources can be used.Aim: This present study is aimed to evaluate the effect of coconut water on fertility in male Wistarrats.Materials and Methods: Coconuts of about 8 months old were harvested from Apata area of...

Common Household Insecticides Used in Nigeria Induced Oxidative Stress in Wistar Rats

Background: The use of household insecticides for the eradication of insects especiallymosquitoes in Nigeria is increasing. These insecticides are used without consideration of theiradverse effect on human health.Aim: This study is therefore sought to investigate the effect of common household insecticidesused in Nigeria on oxidative stress biomarkers.Methodology: Thirty (30) male Wistar rats were divided into five groups of six (6) each. Rats ingroup 1 were exposed to Rambo, those in group 2...

Chemical Content and Antioxidant Potential of Aqueous Extract of Irish Potato Tubers Traditionally Used for Ulcer Treatment in Nigeria

Aim: The study evaluated the chemical contents and antioxidant potential of aqueous extract of Irishpotato tubers traditionally used for ulcer treatment in Nigeria.Methods: Freshly harvested Irish potato tubers were purchased from Aba grocery market, AbiaState. They were thoroughly washed in running water, peeled, sliced and dried in an oven at 60ºC toa constant weight. The dried potatoes were milled into fine powder (flour) and extracted using wateras the solvent. The proximate, mineral, ph...

Bigi Soft Drinks might Induce Hyperglycemia and Hyperlipidemia in Wistar Rats

Background: Bigi soft drinks are carbonated drinks produced by Rite Foods Limited. Thecompany is an indigenous company in Nigeria. Before 2016, Coca-cola bottling company and 7upbottling company products were the dominant soft drinks in Nigeria. Rite Foods Limited introducedcarbonated soft drinks into the Nigerian market in 2016 and have favourably competed with theexisting products. As at today, Bigi soft drinks are the dominant soft drinks in Nigeria because oftheir palatable taste, large v...

Antihypertensive Potential of Coconut (Cocos nucifera L.) Water in Wistar Rats

Background: Hypertension has become a common sickness in Nigeria especially amongpopulation above 50 years of age. It constitutes a major riskfactor for several cardiovasculardiseases suchas atherosclerosis, heart failure, renal insufficiency, coronary artery disease andstroke.Aim: This study was aimed at evaluating the antihypertensive potential of coconut water in Wistarrats.Materials and Methods: Thirty Wistar rats were divided into three groups of ten each. Rats ingroups 1 and 2 received ...

Antifertility Effect of Ethanolic Leaf Extract of Carica papaya in Male Wistar Rats

The use of Carica papaya leaves in folklore medicine for its antimalarial andantidiabetic activities without reference to its adverse effect is on theincrease globally. This study therefore sought to investigate the antifertilityeffect of C. papaya leaves in male Wistar rats. Fresh and healthy leaves of C.papaya disease were harvested from the Institute of Agricultural Researchand Training, Moor Plantation, Ibadan. They were dried and extracted usingsoxhlet apparatus and ethanol as the solven...

Antidiabetic Potential of Alkaloid Extracts from Vitex doniana and Ficus thonningii Leaves on Alloxan-induced Diabetic Rats

Background: The growing number of diabetes coupled with the harsh side effects of somesynthetic drugs has led to the increasing search for alternatives which are relatively cheap withminimal side effects.Aim: This study sought to investigate the antidiabetic potential of alkaloid extracts from Vitexdoniana and Ficus thonningii leaves on alloxan-induced diabetic rats.Methods: Fresh leaves of V. doniana and F. thonningii were obtained from a local market Nkwaguin Abakaliki local government area...

Antidiabetic Effect of Ethanolic Extract of Carica papaya Leaves in Alloxan-Induced Diabetic Rats

The growing number of diabetes coupled with the harsh side effects of some synthetic drugs has led to the increasing search for alternativeswhich are relatively cheap with minimal side effects. This study sought to investigate the antidiabetic effect of ethanolic extract of Carica papaya leavesin alloxan-induced diabetic rats. Thirty adult male albino rats were induced intraperitoneally with alloxan. The rats were grouped into six groups offive animals per group: Group A was not induced with ...

Antibacterial Potential of Ethanolic and Aqueous Extracts of Carica papaya Leaves

Background: The search for newer sources of antibiotics is a global challenge pre-occupyingresearch institutions, pharmaceutical companies and academia, since many infectious agents arebecoming resistant to synthetic drugs.Aim: This present study sought to investigate the antibacterial potential of ethanolic and aqueousextracts of Carica papaya leaves.Materials and Methods: Fresh and healthy leaves of C. papaya were harvested, air dried andmilled into powder. The powder was extracted using et...

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