Research Papers/Topics in Biochemistry

A systematic review on COVID-19 pandemic with special emphasis on curative potentials of Nigeria based medicinal plants

ABSTRACTDespite the frightening mortality rate associated with COVID-19, there is no known approved drug to effectively combat the pandemic. COVID-19 clinical manifestations include fever, fatigue, cough, shortness of breath, and other complications. At present, there is no known effective treatment or vaccine that can mitigate/inhibit SARSCoV-2. Available clinical intervention for COVID-19 is only palliative and limited to support. Thus, there is an exigent need for effective and non-inva...

Hepatoprotective Potential of Alkaloid Extracts from Vitex doniana and Ficus thonningii Leaves in Alloxan-induced Diabetic Rats

Aim: This study sought to evaluate the hepatoprotective potential of alkaloid extracts from V. doniana and F. thonningii leaves on alloxan-induced diabetic rats.Materials and Methods: Fresh leaves of V. doniana and F. thonningii were obtained from a local market Nkwagu in Abakaliki local government area of Ebonyi State. They were dried and pulverized to fine granules using manual grinder. Crude alkaloid was extracted using standard method. The acute oral toxicity of both plants was determined...

Evaluation of Methanolic Extract of Bambara Nut on Renal Indices of Wistar Rats

Background: Bambara nut is a common household food in Nigeria especially among the Igbos ofthe South Eastern part of the country.Aim: This study is aimed at investigating the effect of Bambara nut on renal indices of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powder using a coffee grinder and extracted using soxhlet apparatus and methanol as the so...

Effect of Bambara Nut on Hepatic Biomarkers of Wistar Rats

Aim: This study sought to investigate the effect of Bambara nut on hepatic biomarkers of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powderusing a coffee grinder and extracted using soxhlet apparatus and methanol as the solvent. Twentyfour adult male Wistar rats were acclimatized for seven days during which they were fed ad libitum with standar...

Hepatotoxic effect of Xylopia aethiopica Fruit in Wistar Rats

Background: Xylopia aethiopica fruit has been reported to possess high medicinal value. Thus, people use it without any regard for its toxicity.Aim: This study is therefore aimed at investigating its toxicity on the liver of Wistar rats.Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria and were authenticated. They were air-dried and extracted using Soxhlet apparatus and ethanol as solvent. The median lethal dose (LD50) of the extract was d...

Phytochemical Analysis, Radical Scavenging And Anti-Microbial Activity Of The Leaves Of Gomphocarpus Fruticosus

AbstractGomphocarpus fruticosus is a wild plant that belongs to the Asclepiadaceae family and has many traditional uses. The plant has demonstrated by this study a significant level of microbial and phytochemical activity; the drug-resistant strains of microbes which have emerged in the last decade will be eradicated by the compounds this indigenous plant carries. This particular study aimed to scientifically validate the traditional uses of the plant G. fruticosus. The objectives of this stu...

Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods

ABSTRACTThe phytochemical contents of some milled Nigerian softwood chips were carried out in a quest to evaluate their potentials as sources of alternative medicine as well as uses in other industrial applications. The qualitative and quantitative analysis were ascertained. Tannin was found in all the Nigerian softwoods examined with the highest quantities obtained in Sterculia oblonga (1240 mg/100 g) and Barteria nigritiana (1230 mg/100 g). Highest quantities of alkaloid were obtained in...

EFFECT OF FOOD CHAIN MEDIATED CHRONIC EXPOSURE TO ARSENIC AND CADMIUM ON REDUCED GLUTATHIONE (GSH) AND GLUTATHIONE S￾TRANSFERASE (GST) LEVELS IN THE BRAIN OF ALBINO RAT (WISTAR RAT)

ABSTRACTThe study aims to evaluate the effect of food chain mediated chronic exposure to arsenic and cadmium (singly and combined) on the level of reduced glutathione (GSH) and the activity of glutathione S-transferase (GST) in brains of rats. Fortymale Wistaralbino rats were fed with a diet containing fish fed with normal feed in water contaminated with 0.4 mg/100 ml cadmium (group B), arsenic (group C) and cadmium + arsenic (group D) respectively for three months. The control rats were...

Hypolipidemic Propensity of Ethanolic Extract of Xylopia

ABSTRACT  Aim:  This  study  is  aimed  at  assessing  the  effect  of  Xylopia  aethiopica  fruit  on  the  lipid  profile  of Wistar rats. Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria  and  were  authenticated.  They  were  air-dried  and  extracted  using  Soxhlet  apparatus  and etha...

Weight Gain Reduction and Hypoglycemic Effects of Xylopi

ABSTRACT  Aim: This study  is aimed  at determining the effect of Xylopia  aethiopica  fruit on weight gain and blood sugar level of Wistar rats. Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria  and  were  authenticated.  They  were  air-dried  and  extracted  using  Soxhlet  apparatus  and ethanol  as  solvent.  The  median  let...

Nephrotoxicity of Ethanol Extract of Xylopia aethiopica

  ABSTRACT  Background: The  use  of  Xylopia  aethiopica  fruit  in  folklore  medicine  is  on  the  increase  without regard to its toxicity. Aim: This study was aimed at examining its toxicity on the kidney of Wistar rats. Methodology:  The  fruits  of  Xylopia  aethiopica  were  air-dried  and  extracted  using  Soxhlet apparatus  and  ethanol  ...

Hybridoma Technology

  INTRODUCTION: Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies ). This process starts by injecting a mouse (or other mammal) with an antigen that provokes an immune response. A type of white blood cell, the B cell, produces antibodies that bind to the injected antigen. These antibody producing B-cells are then harvested from the mouse and, in turn, fused with immortal B cell cancer cells, a myeloma ,  to prod...

Garlic Bulb Prevents High Blood Pressure in Egg Yolk induced Hypertensive Rats

The aim of this study is to access the antihypertensivepotential of garlic juice in rats. To achieve this, 30 Wistar ratswere divided into three groups of 10 each. Rats in groups 1 and 2received aspecial prepared egg feed diet (24 egg yolkmixed with 1kg of normal rat diet) while those in group 3 received normalfeed diet and they served as the control group. Animals in group2 were administered 3 mL/100g body weight of undiluted garlicjuice simultaneously with the feeding. The animals were fedo...

Egg Yolk induces High Blood Pressure in Wistar Rats

Hypertension constitutes a major risk factor forseveral cardiovascular diseases such asatherosclerosis, heart failure, renal insufficiency,coronary artery disease and stroke. The risk factorincreases with age in both sexes. According to WHO,about one-third of the world’s population suffersfrom hypertension and the incidence has beenincreasing at a rapid rate due to life stylemodification. This study is aimed at investigating theeffect of egg yolk on blood pressure of Wistar rats. Toachieve ...

Effect of Consumption of Garri Processed by Traditional and Instant Mechanical Methods on Lipid Profile of Wistar Rats

Aim: This study focuses on the effect of consumption of garri processed by traditional and instantmechanical methods on the lipid profile of Wistar rats.Methods: Cassava samples were obtained from the International Institute of Tropical Agriculture(IITA), Ibadan and were processed using traditional and instant mechanical methods. Fifteen adultmale Wistar rats were purchased from the Animal Holding Unit of the Department of Physiology,University of Ibadan, Nigeria with body weight between 100 ...


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