Biochemistry Research Papers/Topics

STIP1/HOP Regulates the Actin Cytoskeleton through Interactions with Actin and Changes in Actin-Binding Proteins Cofilin and Profilin

Abstract Cell migration plays a vital role in both health and disease. It is driven by reorganization of the actin cytoskeleton, which is regulated by actin-binding proteins cofilin and profilin. Stress-inducible phosphoprotein 1 (STIP1) is a well-described co-chaperone of the Hsp90 chaperone system, and our findings identify a potential regulatory role of STIP1 in actin dynamics. We show that STIP1 can be isolated in complex with actin and Hsp90 from HEK293T cells and directly interacts wit...

PHYTOCHEMICAL CONSTITUENTS OF SOME MEDICINAL PLANTS FOUND IN KAPTUMO DIVISION IN NANDI COUNTY, KENYA

Abstract Medicinal plants are widely used by the traditional healers to prevent or treat various human diseases since time immemorial due to the chemical substances found in them. The phytochemical constituents of most of these plants has not been determined hence the present study conducted at Kaptumo Division in Nandi County seeks to profile the phytochemicals found in medicinal plants namely: Kigelia africana (Lam.) and Benth, Ekebergia capensis Sparrm and Fagaropsis angolensis (Engl.) Da...

Molecular Characterization of Indigenous Rhizobia from Kenyan Soils Nodulating with Common Beans

Abstract : Kenya is the seventh most prominent producer of common beans globally and the second leading producer in East Africa. However, the annual national productivity is low due to insufficient quantities of vital nutrients and nitrogen in the soils. Rhizobia are symbiotic bacteria that fix nitrogen through their interaction with leguminous plants. Nevertheless, inoculating beans with commercial rhizobia inoculants results in sparse nodulation and low nitrogen supply to the host plants b...

Detection of trypanosomes in small ruminants and pigs in western Kenya: important reservoirs in the epidemiology of sleeping sickness?

Abstract Background: Trypanosomosis is a major impediment to livestock farming in sub-Saharan Africa and limits the full potential of agricultural development in the 36 countries where it is endemic. In man, sleeping sickness is fatal if untreated and causes severe morbidity. This study was undertaken in western Kenya, an area that is endemic for both human and livestock trypanosomosis. While trypanosomosis in livestock is present at high levels of endemicity, sleeping sickness occurs at low...

Characterization of algae oil (oilgae) and its potential as biofuel in Kenya

Abstract A survey of microalgae biodiversity in three Kenyan Rift valley lakes identified high oil yielding species abundantly distributed naturally. The species were cultured in BBM and BG-11 media to obtain pure clones and lipids (oil) extracted by the Bligh and Dyer method. The peak lipid content ranged from 1.5 – 10.5% of algal biomass with Chlorella species showing the highest yields (10.5%), followed by Euglena acus (5.88), Nitzschia (3.68%), Ankistrodesmus falcatus (1.58%) and Scene...

Analysis of genetic diversity in Eucalyptus grandis (Hill ex Maiden) seed sources using inter simple sequence repeats (ISSR) molecular markers

Abstract Eucalyptus grandis is an economically important tree species that is native to the Australian continent and its northern neighbours, where it is grown primarily for its hard wood timber and pulp for paper industries. It is widely grown in tropical countries such as South Africa, Kenya, Angola, Ghana, and Zimbabwe. Five ISSR primers generated 41 scorable polymorphic bands which were used to analyse genetic diversity between and within the seed sources and for construction of neighbou...

Synthesis, characterization of nitro or amino substituted pyridyl ligands bridged by an ester or ether bond, and their antibacterial assessment against drug resistant bacteria.

Abstract: A series of ester and ether bridged compounds bearing the nitro and amino moieties were synthesized and characterized, in order to assess their ability to potentially serve as antibacterial agents against drug resistant bacteria. All the compounds were obtained at considerable yields while characterization techniques confirmed their successful synthesis. Furthermore, their antimicrobial assessments showed that all the compounds exhibited antibacterial activity against the Gram-nega...

Polyphenols as Potential Indicators for Drought Tolerance in Tea (Camellia sinensis L.)

Abstract: Plant polyphenols have gained prominence in quality of plant products and in human health. an experiment...(continue from the attached pdf)

Polypyrrole Based Biofunctional Composite Layer for Bioelectrocatalytic Device System

The involvement and development of intelligent polymers in enzymes as a catalyst have been interesting to develop processes that are environmentally benign, energy efficient, and selective towards their specific molecular applications. Polypyrrole based enzyme nanocomposites represents a continuous considerable redox organic polymer  that have been increased in electromechanical devices in advance  for  surface  functionalization. This review addresses the fundamental concepts of poly...

Sex-moderated relationship between the 2D:4D ratio and circulating hormones in an adult Ghanaian population

The second-to-fourth digit ratio (2D:4D) is the putative marker of prenatal hormone exposure. The 2D:4D ratio or the right–left difference (Dr-l) are said to be negative and positive correlates, respectively, of circulating testosterone and estrogen in both adult males and females. However, previous studies on the subject have reported mixed results. This study aimed to determine the sex-moderated relationship between the 2D:4D ratio and adult circulating testosterone, estradiol, testostero...

Interactions between sex and the age at disease onset on cardiometabolic risk factors in a Ghanaian population with type 2 diabetes mellitus: A cross‐sectional study

Background and Aim: The are sex differences in cardiometabolic risk factors in type 2 diabetes mellitus (T2DM) as well as the age at disease onset. However, the impact of these risk factors on the age at onset of T2DM is less known in the Ghanaian population. An understanding of the differential impact of cardiometabolic risk factors on the age at onset on T2DM may lead to sex‐specific interventions in preventive and management strategies for T2DM. Methods: The study was cross‐sectional f...

The Impact of Disease Duration on Sex Differences in Cardiometabolic Risk Factors in Type 2 Diabetic Patients on Metformin-glimepiride Oral Therapy

Aims: Patients with type 2 diabetes mellitus (T2DM) are commonly treated with metformin-glimepiride combination therapy. The relationship between disease duration and cardiometabolic risk factors among T2DM patients on metformin-glimepiride combination therapy may be moderated by sex. This, however, has not been investigated in the Ghanaian population. Study Design: This was a cross-sectional study. Place and Duration of Study: The study was conducted from January to December 2019 at the Bol...

Insulin Resistance in relation to Hypertension and Dyslipidaemia among Men Clinically Diagnosed with Type 2 Diabetes

Pathophysiologically, type 2 diabetes can result from insulin resistance or insulin insufficiency alone. It is unclear whether relative insulin shortage or pronounced insulin resistance is linked to poor cardiometabolic problems like obesity. Therefore, the objective of this study was to evaluate the relationship between insulin resistance (IR), hypertension, and dyslipidaemia, in men with type 2 diabetes mellitus. One hundred and twenty-one (121) type 2 diabetic men participated in this cros...

Phytomedical assessment of two Cymbopogon species found in Nkonkobe Municipality: toxicological effect on human Chang liver cell line

Abstract Background: Cymbopogon species are widely used as herbal remedies by the traditional healers living in Nkonkobe Municipality for the treatment and management of skin and respiratory infections. According to our survey, the plants seem to be very important because of the higher demands. Methods: The leaves of C. validis and C. plurinodis were hydro-distilled and the resulted extracted oils were analyzed by GC/MS. Minimum inhibitory concentrations (MICs) ranging from 7.8 to 500.0 μg/m...

Comparative Diagnostic Techniques for Cryptosporidium Infection

Abstract: Diarrhoea caused by Cryptosporidium is usually mild in immune competent individuals but severe in the young and those with underlying disease leading to compromised immunity. The conventional diagnosis of Cryptosporidium requires observation of the infective oocysts however, their tiny size yields indistinct results, thus limiting the effectiveness of the conventional diagnostic technique, modified Ziehl-Neelsen (ZN) differential staining. Consequent to the abovementioned limitatio...


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