Biochemistry Research Papers/Topics

Isolation And Characterization Of Cellulolytic Bacteria From Soils Excavated From Geothermal Wells Along The Kenyan Rift Valley

Abstract: Cellulolytic microorganisms such as fungi and bacteria are responsible for much of the cellulose degradation in soils. Despite this vast number of cellulase producers, there is a deficiency of microorganisms that can produce significant amount of the three cellulase enzyme specifities i.e. endoglucanases, exoglucanases and cellobiases to efficiently degrade cellulose to fermentable products. Little emphasis has been given to cellulase production from bacteria despite their extremel...

Molecular characterization and genetic diversity of tomato root-knot nematodes (Meloidogyne spp.) from selected localities of Kenya

Abstract: Distinguishing pathogenic nematodes on the basis of morphological characters is difficult because differences in characters may be absent or difficult to observe. Hence identification to the species level is increasingly reliant on molecular based techniques. Polymerase Chain Reaction (PCR) based techniques offer possibilities for precision, sensitivity and quantification. Tomato root-knot nematodes from the three selected areas in Kenya were characterized by analysis of isozyme ph...

Prevalence and Genetic Diversity of Simian Immunodeficiency Virus Infecting free-ranging non-human Primates in Kenyan Urban Centres

Abstract: My foremost profound gratitude goes to God Almighty who has granted me good health,strength and ability to accomplish this study and more so during the challenging academic times. Second, I am grateful to my supervisors Dr. Maamun Jeneby and Dr. Vincent Owino Adung’a for their time, guidance, dedication and involvement in my academic pursuits. Special thanks to Dr. Maamun and Dr. Mercy Akinyi who were kind enough to offer an opportunity to conduct a research project for my thesis...

Prevalence and transmission of selected infectious disease-causing enzootic and zoonotic pathogens by haematophagous camel keds, Hippobosca camelina, in Laisamis, Marsabit county of northern

Abstract: Hippobosca camelina, also known as camel keds or camel louse flies, are the most common blood-sucking ectoparasites infesting camels in, Laisamis Ward, northern Kenya. The ability of these obligate haematophagous biting flies to transmit infectious agents is less understood. The aims of this study were to; I) screen for protozoan, bacterial and viral pathogens in camels and their biting keds, H. camelina, sampled from Laisamis Ward, Marsabit county II) assess the potential utility ...

Determination Of Ascorbic Acid In Vitamin C Syrups Sold In Gombe Metropolis

ABSTRACT    Titrimetric method was used to analyze ten different brands of each vitamin C syrups that are sold in Gombe metropolis. The percentage of ascorbic acid content in vitamin C syrups containing 100mg/5ml showed that samples (1,2,3 and 7) having (68.37%,54.10%, 52.10%, and 74.00%) were below 95-105 recommended by British pharmacopeia limit. While for A samples (4, 5 and 6)have percentage ascorbic acid content of (95.00%, 95.10% and 96.00% )respectively and were within the normal ran...

Characterization of Trypanosomes Isolated from Bovine and Canine Using Polymerase Chain Reaction.

ABSTRACT Trypanosomiasis is a vector-borne parasitic disease, transmitted to animals by tsetse fly (Glossina genus) bites. The human type causes sleeping sickness in man while animal type causes nagana in animals. The aim of this study is to characterize trypanosomes isolated from dogs and cattle in Jos South Abattoir. Isolated trypanosomes were subjected to polymerase chain reaction using kin primers targeting internal transcribed spacer 1 (ITS1) of ribosomal deoxyribonucleic acids. The PCR ...

Effect of a Mixture of Allium Cepa and Camellia Sinensis Extracts On Hyperlipidemia Male Wistar Rats

ABSTRACT The burden of hyperlipidemia is on the rise globally especially in many low income countries like Uganda. Management of this metabolic disorder mainly involves dietary and behavioral therapies, which are often met with poor results as they require time and discipline from the patients. The chemotherapeutic options available are expensive, are associated with many side effects and are not readily available to the average citizen. Thus, an alternative effective remedy which is readily ...

Effect of a Mixture of Allium Cepa and Camellia Sinensis Extracts On Hyperlipidemic Male Wistar Rats

ABSTRACT The burden of hyperlipidemia is on the rise globally especially in many low income countries like Uganda. Management of this metabolic disorder mainly involves dietary and behavioral therapies, which are often met with poor results as they require time and discipline from the patients. The chemotherapeutic options available are expensive, are associated with many side effects and are not readily available to the average citizen. Thus, an alternative effective remedy which is readily ...

Common Mitochondrial DNA Mutations Among Infertile Males On Referral to The Korle-Bu Teaching Hospital

ABSTRACT Background: Studies to find the association between male infertility and mtDNA mutations have been carried out by many researchers but reports from these studies have been varied and contradictory at times. However studies of this nature are scarce in Ghana. The present study has therefore provided a baseline data on mtDNA mutations in relation to male infertility for further studies to be carried out in this area. Main Objective: The overall objective is to ascertain the common mtD...

Effect of Chronic Exposure to Petroleum Hydrocarbon Pollution On Some Biochemical Parameters of Individuals Native to Ebocha, Niger Delta

ABSTRACT The impact of pollution due to gas flaring on individuals native to Ebocha in Niger Delta Area was investigated. One hundred and five apparently healthy individuals between 21-70years who consented for the study were screened, seventy five individuals from Ebocha and thirty from Nsukka-a control location with no history of petroleum hydrocarbon pollution. Standard analytical procedures were used in the determination of the concentration of ascorbic acid (AA) of the individuals (males...

Studies On Possible Beneficial Biochemical Outcomes of Single and Combinatorial Herbal Formulations On Diabetic Wistar Rats

ABSTRACT Medicinal plants continue to provide valuable therapeutic recipes, in orthodox and traditional healing systems, for the amelioration of diseases and disorders. The current study sought to evaluate the capacities of single herbal formulations (SHFs), double herbal formulations (DHFs), triple herbal formulations (this) and quadruple herbal formulation (QHF) of leaf extracts of Acanthus Montanus, Asystasia gangetica, Gongronema latifolium and Solanum melongena to ameliorate hyperglycem...

Chemo protective Effect of Methanol Leaf Extract of Picralima Nitida On Carbon Tetrachloride-Induced Oxidative Stress in Albino Rats

ABSTRACT Among medicinal plants, Picralima nitida is well investigated and has been shown to possess renal and hepatoprotective activities. The present study was conducted to investigate the chemoprotective effect of methanol extract of Picralima nitida leaves on carbon tetrachloride-induced oxidative stress in albino rats. Thirty (30) Wistar albino rats of six (6) rats per group were used for this study and were divided into 5 groups. Group 1 rats were normal control; group 2 (negative cont...

Chemical Contents and Characterization of the Biologically Active Compounds of Nigella Sativa Linn. (Ranunculaceae) Seeds

ABSTRACT The seed of Nigella sativa was evaluated for its chemical composition, medicinal as well as its nutritional values. The powdered seed was extracted with methanol to give the crude extract. Phytochemical screening of the crude extract revealed the presence of the following classes of natural products; alkaloids, tannins, flavonoids, saponins and anthraquinones, sterols and terpenes. The proximate analysis showed 12.00% moisture, ash content 3.00%, ether soluble extract 33.30% crude p...

Biochemical Effect of Ethanol Leaf Extract of Mucuna utilis on Lead Acetate Induced Toxicity on Wistar Albino Rats

ABSTRACT This study investigated the effect of ethanol leaf extract of Mucuna utilis (EEMu) on lead acetate induced toxicity on Wistar albino rats. Acute toxicity analysis of the plant extract produced no lethality even at high doses of 3000 mg/kg body weight. Twenty albino rats weighing 115 – 235 g were divided into four (4) groups; the first groupof animals served as normal control and were not administered either lead acetate or EEMU. The second group was treated intraperitoneally with ...

Assessment of Phytochemical and Nutrient Compositions of Edible Yam Species in Southeastern Nigeria

ABSTRACT This study assessed the phytochemical and nutrient compositions of edible yam species Dioscorea rotundata 1, Dioscorea rotundata 2, Dioscorea rotundata 3, Dioscuri rotundata 4, Dioscorea Cayenensis, Dioscorea alata, Dioscorea dumentorum and Dioscorea bulbifera in Southeastern Nigeria. The phytochemical and nutrient (proximate, minerals, vitamins, fatty acids and amino acids) compositions of the yam tubers were investigated using standard analytical methods. 


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