Enzyme Assay, Purification and Cell Disruption
Hexokinase and glucose oxidase
Uricase and cholesterol esterase
NEED FOR ENZYME PURIFICATION
STEPS IN ENZYME PURIFICATION
Microbial source
Heterologous enzymes in microbes
Plant source
Animal source
Methods for initial recovery
Removal of cell debris and whole cells
Removal of nucleic acids and lipids
Concentration of enzymes
Final purification by column chromatography
Consults, E. (2022). BCH 303 - Introduction to Enzymology. Afribary. Retrieved from https://afribary.com/works/bch-303-introduction-to-enzymology
Consults, Education "BCH 303 - Introduction to Enzymology" Afribary. Afribary, 23 Sep. 2022, https://afribary.com/works/bch-303-introduction-to-enzymology. Accessed 18 May. 2024.
Consults, Education . "BCH 303 - Introduction to Enzymology". Afribary, Afribary, 23 Sep. 2022. Web. 18 May. 2024. < https://afribary.com/works/bch-303-introduction-to-enzymology >.
Consults, Education . "BCH 303 - Introduction to Enzymology" Afribary (2022). Accessed May 18, 2024. https://afribary.com/works/bch-303-introduction-to-enzymology