Microbiology Research Papers/Topics

Prevalence Ofci-Ilamydia Trachoma Tis Igm In Students Of Federal University Oye-Ekiti, Ek.It! State, Nigeria

ABSTRACT Chlamydia trachomatis infections are the most common bacterial cause of sexually transmitted diseases (STDs) in the world. This study was to investigate the prevalence of Chlamydia trachomatis infection amongst students of Federal university Oye-Ekiti, Ekiti State, Nigeria. Urine samples were collected from ninety four participants and were screened using the microwell ELISA Chlamydia trachomatis kit - Swab/Urine . (Golden Bio Technologies Corp, USA). Out of 94 patients screened, 26...

Production Of Alpha Amylase Using Fungus From.The Rhizosphere Of Moringa Oleif'era .

ABSTRACT Alpha amylase enzyme (EC 3.2..1. 1) (a-1, 4 glucan-glucanohydrolase) is widely distributed in nature such as plants, animals and microorganisms. Production of extracellu Jar alpha-amylase from fungi was isolated from rhizosphere of Moringa oleifera. Three (3) fungi isolates were selected after serial dilution of the soil samples, four optimization parameters was employed to determine the enzyme activity and they include effect of inoculum size, pH, nitrogen source and surfactant·on...

Antibacterial Effect Of Zingiber Officinale On Staphylococcus Aureus

.ABSTRACT The growing population concern about health has recently led to the development of natural antimicrobials to control diseases. Medicinal plants and spices are one of the most commonly used natural antimicrobial agents in food and have been used traditionally for thousands of years by many cultures for controlling common health. Zingiber ojjicinale has long been used as naturopathy due to their potential antimicrobial activity against different microbial pathogens. This study was co...

Prey Alence Of Dengue Virus In Mosquitoes Caught In Oye Ekiti, Ekiti State, Nigeria

ABSTRACT Dengue Virus is the most prevalent arthropods borne viral infection in tropical and sub-tropical regions worldwide and they are transmitted by Aedes aegypti and Aedes albopictus. This experiment was conducted with the aim of detecting and establishing possible sylvatic transmission of Dengue Virus in mosquitoes caught in Oye Ekiti. Mosquito life traps were used to trap 200 Aedes mosquitoes which were then knocked down by the use of insecticides. RNA were extracted from the mosquitoe...

Assay Of Antibiotic Producing Microorganisms From Different Soils In The Premises Of Federal University Oye-Ekiti, Ekiti St A Te. (Fuoye)

ABSTRACT A total of eight soil samples were collected from different sites within FUOYE environment and were analyzed to determine the presence of antibiotic producing bacteria, fungi and actinomycete Using nutrient agar, sabauraud dextrose agar and glycerol yeast extract agar as culture media. Seven bacteria: Escherichia coli, Bacillus spp, Pseudomonas spp, Azomonas spp, Gluconobacter spp, Micrococcus spp, and Staphylococcus aureus; five fungi: Rhizopus spp, Mucor spp, Rhodotorula spp, ...

Detection Of Bioactive Compounds By Bioautography Of Diodia Scandens On Staphylococcus Aureus And Coagulase Negative Staphylococcus

ABSTRACT Plant samples collected within and around Federal University Oye-Ekiti was air dried, pulverized and subjected to hot (soxhlet) extraction and cold extraction using three (3) extractive solvent (ethyl acetate, methanol, n-hexane).' The extracts were seperated from the solvent using rotary evaporator while the crude extracts were subjected to thin layer chromatography (TLC) using different solvent ratio mixtures on a silica gel coated TLC plates. The antibacterial activity of act...

Detection Of Bioactive Constituents In Phyllanthus AmarUs Using Thin Layer Chromatography-Bioautographic Method Against Staphylococcus Aureus And Coagulase Negative Staphylococcus AUreUs

ABSTRACT Medicinal plants have been used for centuries as remedies for human diseases because they contain components of therapeutic value. Phyllanthus amarus has shown to work as an antifungal, antibacterial and antiviral agent. Bioautography is a technique used to isolate compounds by employing a suitable chromatographic process followed by a biological detection system. Referenced pure culture antibiotics resistant Coagulase Negative Staphylococcus strain (Co NS I Ob: Ojo) and Staphyl...

Prevalence Of Human Papilloma Virus (Lgm) Antibody Among Fuoye Student

ABSTRACT Human papilloma virus (HPV) infections are the most common sexually transmitted infections(STDs) in the world. This study was to document the prevalence. of Human papilloma virus infection in Federal university Oye-Ekiti, Ekiti State, Nigeria. Urine samples were collected from ninety four participants and were screened for HPV (lgM) antibody using the HPV ELISA. Out of 94 samples analysed, 2 (2.1 %) was positive for Human papilloma virus IgM, and higher prevalence were recorded i...

Detection of Cna, Ica, Hlg and SdrE Virulent genes from Staphylococcus aureus Isolates in Khartoum State

ABSTRACT S. aureus is an extraordinarily versatile pathogen, and it can cause a large spectrum of infections, from mild to severe and fatal. It is important in humans and also economically important when infecting animals, able to cause superficial lesions and systemic infections. The aim of this study is to detect Can, Ica, Hlg and SdrE virulent genes of Staphylococcus aureus associated with different types of infections among Sudanese in Khartoum State. The Samples were collected from dif...

Isolation And Identification Of Microorganisms Associated With Grounded Palm Kernel Shells Using Chicken Droppings As Innoculum At . Ratio 2:1.

ABSTRACT This research study is designed to determine the microbial count of Chicken Droppings and Ground Palm kernel shell in which the chicken droppings is used as inoculum on Ground Palm Kernel Shell at Ratio 2: l in which the inoculum size is half of the Ground Palm Kernel Shell. Pollution of the environment due to oil production can be reduced through this study because is aimed at examining the microorganism responsible for the biodegradation that can be achieved in Palm kernel she...

PROPHYLACTIC ACTIVITY OF Phyllantlms Amarus And Diodia Scandens IN SYNERGISM O SWI S ALBINO RATS

ABSTRACT This study is aimed at evaluating the proP,hylactic activity of Phy/lanthus amarus and Diodia scandens in synergism on the Swiss albino rats. The two plants were obtained from Federal University Oye-Ekiti and its environs, thoroughly washed with distilled water and air dried at room temperature for two weeks. The air dried plant materials were pulverized into powdery form using house-hold electric blender. Ethyl-acetate extracts of the two plants combined was administered orally in ...

Prevalence Of Malaria Among Patients With Sickle Cell Trait In Ekiti State

ABSTRACT This study was carried out to determine the prevalence of malaria parasite among patients with sickle cell trait in Ekiti state. A total of 20 samples were collected from different hospitals and clinics. The samples were collected randomly and sodium metabilsulphite was employed to confirm the sickling trait in the samples and thereafter the malaria parasite kit was used to test for the prevalence of malaria in the samples. Eight (8) persons who tested positive for sickle cell trait...

Antibiotic Susceptibility Pattern Of Aerobic Mesophilic Bacteria Isolated From Kunu Samples Sold In Ile-Ife, Osun State, Nigeria.

ABSTRACT The antibiotic susceptibility pattern of aerobic mesophilic bacteria isolated from kunu samples sold in Ile-lfe, Osun State, Nigeria was studied following standard microbiological methods. Seven ready-to-drink kunu samples were purchased from different hawkers at Ile Ife. The samples were transported immediately in ice bucket to the laboratory for analysis. The aerobic mesophilic bacteria associated with kunu samples were isolated, labeled and identified by phenotypic and biochemica...

In Vitro Antimicrobial Activity Of Methanolic Extract Of Cymbopogon Schoenanthus Against Bacteria Isolated From Urinary Tract Infected Patients In Khartoum

Abstract Medicinal plants have been widely used in folk medicine. They are considered today as an interesting source of new drug due to their bioactive components. The genus, Cymbopogon schenanthus (Poaceae). is an aromatic plant seems important source of several bioactive compound use in traditional medicine . This was descriptive and cross sectional study conducted during the period from June to October 2018, to determine the antimicrobial activity of Cymbopogon schoenanthus methanolic ext...

Antimicrobial And Antioxidant Activity Of Rosmarinus Officinalis L. Flowers Pods And Thymus Vulgaris L. Leaves Ethanolic Extract On Escherichia Coli Urinary Isolates

ABSTRACT The indiscriminate use of antibacterial agents has led to one of the largest recent global health problems which is the emergence of bacterial resistance. The present study was aimed to investigate the antimicrobial and antioxidant activity of ethanolic extracts of the two medicinal plants; Rosmarinus officinalis L. and Thymus vulgaris L.on Escherichia coli (E.coli). Antibiotic susceptibility test was applied against E.coli urinary isolates using Chloramphenicol, Gentamycin, Amoxici...


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