TABLE OF CONTENTS
DECLARATION ............................................................................................................................ i
DEDICATION ............................................................................................................................... ii
APPROVAL ................................................................................................................................. iii
ACKNOWLEDGMENT ......................................... , ................................................................... iv
LIST OFT ABLES ...................................................................................................................... vii
ABBREVIATIONS .................................................................................................................... viii
CHAPTER ONE ........................................................................................................................... 1
1.0 INTRODUCTION ................................................................................................................. 1
1.1 BACKGROUND INFORMATION ....................................................................................... I
1.2 Statement of the pro b !em ...................................................................................................... 2
1.3 Justification for the study ...................................................................................................... 2
1.4 Research questions ............................................................................................................ 3
1.5.2 Specific objectives .......................................................................................................... 3
CHAPTER TWO .......................................................................................................................... 4
2.0 LITERATURE REVIEW ...................................................................................................... 4
2.5 Methods for antibacterial susceptibility ................................................................................ 8
2.6 Minimum Inhibitory Concentration .................................................................................... l 0
2. 7 Minimum Bactericidal Concentration ................................................................................. I 0
CHAPTER THREE ...................................................................................................................... 11
3.0 MATERIALS AND METHODS ......................................................................................... 11
3.1 Study Design .. . . . .. .. . . .. ll
3.2 Study area ............................................................................................................................ ll
3.3 Plant collection and identification ....................................................................................... 11
3.4 Storage, drying and pulverization ....................................................................................... 11
3.5 Plant extraction .................................................................................................................... 12
3.6 Determination of extract yield(% yield) ............................................................................. 12
3.8 Determination of Minimum Inhibitory Concentration (MIC) using Broth dilution method ·······································15
3.9 Determination of Minimum Bactericidal Concentration (MBC) ........................................ 16
3.10 Quality control ................................................................................................................... 17
3.14 Data analysis ..................................................................................................................... 18
3.15 Ethical considerations ....................................................................................................... 18
CHAPTER FOUR ....................................................................................................................... 19
4.0 RESULTS ............................................................................................................................ 19
4.1 The results for susceptibility test of Shigella dysenteriae to ethanol whole herb extract of Phyllanthus amarus ........................................... 19
CHAPTER FIVE ........................................................................................................................ 23
5.0 DISCUSSION, CONCLUSION AND RECOMMENDATIONS ...................................... 23
5.1 DISCUSSION ..................................................................................................................... 23
5.2 CONCLUSION ................................................................................................................... 25
5.3 RECOMENDATIONS ........................................................................................................ 26
REFERENCES ............................................................................................................................ 27
APPENDICES ............................................................................................................................. 30
APPENDIX 1: TIME FRAMEWORK AND WORK PLAN ................................................. 30
APPENDIX 2 ............................................................................................................................... 31
Figure I: susceptablity testing agar well diffusion.( zones of inhibition) ................................. 3 I
Consults, E. (2022). Determination of Antibacterial Activity of Whole Plant Extract of Phyllantltus Amarus Schum and Thonn (Kabamba Maliba) against Shigella Dysenteriae.. Afribary. Retrieved from https://afribary.com/works/determination-of-antibacterial-activity-of-whole-plant-extract-of-phyllantltus-amarus-schum-and-thonn-kabamba-maliba-against-shigella-dysenteriae
Consults, Education "Determination of Antibacterial Activity of Whole Plant Extract of Phyllantltus Amarus Schum and Thonn (Kabamba Maliba) against Shigella Dysenteriae." Afribary. Afribary, 15 Sep. 2022, https://afribary.com/works/determination-of-antibacterial-activity-of-whole-plant-extract-of-phyllantltus-amarus-schum-and-thonn-kabamba-maliba-against-shigella-dysenteriae. Accessed 02 Nov. 2024.
Consults, Education . "Determination of Antibacterial Activity of Whole Plant Extract of Phyllantltus Amarus Schum and Thonn (Kabamba Maliba) against Shigella Dysenteriae.". Afribary, Afribary, 15 Sep. 2022. Web. 02 Nov. 2024. < https://afribary.com/works/determination-of-antibacterial-activity-of-whole-plant-extract-of-phyllantltus-amarus-schum-and-thonn-kabamba-maliba-against-shigella-dysenteriae >.
Consults, Education . "Determination of Antibacterial Activity of Whole Plant Extract of Phyllantltus Amarus Schum and Thonn (Kabamba Maliba) against Shigella Dysenteriae." Afribary (2022). Accessed November 02, 2024. https://afribary.com/works/determination-of-antibacterial-activity-of-whole-plant-extract-of-phyllantltus-amarus-schum-and-thonn-kabamba-maliba-against-shigella-dysenteriae