ABSTRACT Trypanosomiasis is a vector-borne parasitic disease, transmitted to animals by tsetse fly (Glossina genus) bites. The human type causes sleeping sickness in man while animal type causes nagana in animals. The aim of this study is to characterize trypanosomes isolated from dogs and cattle in Jos South Abattoir. Isolated trypanosomes were subjected to polymerase chain reaction using kin primers targeting internal transcribed spacer 1 (ITS1) of ribosomal deoxyribonucleic acids. The PCR ...
ABSTRACT The burden of hyperlipidemia is on the rise globally especially in many low income countries like Uganda. Management of this metabolic disorder mainly involves dietary and behavioral therapies, which are often met with poor results as they require time and discipline from the patients. The chemotherapeutic options available are expensive, are associated with many side effects and are not readily available to the average citizen. Thus, an alternative effective remedy which is readily ...
ABSTRACT The burden of hyperlipidemia is on the rise globally especially in many low income countries like Uganda. Management of this metabolic disorder mainly involves dietary and behavioral therapies, which are often met with poor results as they require time and discipline from the patients. The chemotherapeutic options available are expensive, are associated with many side effects and are not readily available to the average citizen. Thus, an alternative effective remedy which is readily ...
ABSTRACT Background: Studies to find the association between male infertility and mtDNA mutations have been carried out by many researchers but reports from these studies have been varied and contradictory at times. However studies of this nature are scarce in Ghana. The present study has therefore provided a baseline data on mtDNA mutations in relation to male infertility for further studies to be carried out in this area. Main Objective: The overall objective is to ascertain the common mtD...
ABSTRACT The impact of pollution due to gas flaring on individuals native to Ebocha in Niger Delta Area was investigated. One hundred and five apparently healthy individuals between 21-70years who consented for the study were screened, seventy five individuals from Ebocha and thirty from Nsukka-a control location with no history of petroleum hydrocarbon pollution. Standard analytical procedures were used in the determination of the concentration of ascorbic acid (AA) of the individuals (males...
ABSTRACT Medicinal plants continue to provide valuable therapeutic recipes, in orthodox and traditional healing systems, for the amelioration of diseases and disorders. The current study sought to evaluate the capacities of single herbal formulations (SHFs), double herbal formulations (DHFs), triple herbal formulations (this) and quadruple herbal formulation (QHF) of leaf extracts of Acanthus Montanus, Asystasia gangetica, Gongronema latifolium and Solanum melongena to ameliorate hyperglycem...
ABSTRACT Among medicinal plants, Picralima nitida is well investigated and has been shown to possess renal and hepatoprotective activities. The present study was conducted to investigate the chemoprotective effect of methanol extract of Picralima nitida leaves on carbon tetrachloride-induced oxidative stress in albino rats. Thirty (30) Wistar albino rats of six (6) rats per group were used for this study and were divided into 5 groups. Group 1 rats were normal control; group 2 (negative cont...
ABSTRACT The seed of Nigella sativa was evaluated for its chemical composition, medicinal as well as its nutritional values. The powdered seed was extracted with methanol to give the crude extract. Phytochemical screening of the crude extract revealed the presence of the following classes of natural products; alkaloids, tannins, flavonoids, saponins and anthraquinones, sterols and terpenes. The proximate analysis showed 12.00% moisture, ash content 3.00%, ether soluble extract 33.30% crude p...
ABSTRACT This study investigated the effect of ethanol leaf extract of Mucuna utilis (EEMu) on lead acetate induced toxicity on Wistar albino rats. Acute toxicity analysis of the plant extract produced no lethality even at high doses of 3000 mg/kg body weight. Twenty albino rats weighing 115 – 235 g were divided into four (4) groups; the first groupof animals served as normal control and were not administered either lead acetate or EEMU. The second group was treated intraperitoneally with ...
ABSTRACT This study assessed the phytochemical and nutrient compositions of edible yam species Dioscorea rotundata 1, Dioscorea rotundata 2, Dioscorea rotundata 3, Dioscuri rotundata 4, Dioscorea Cayenensis, Dioscorea alata, Dioscorea dumentorum and Dioscorea bulbifera in Southeastern Nigeria. The phytochemical and nutrient (proximate, minerals, vitamins, fatty acids and amino acids) compositions of the yam tubers were investigated using standard analytical methods.
ABSTRACT Eukaryotic pathogens are responsible for several serious human diseases, however, most drugs used as treatment regimens are ineffective while others still in use are toxic. This study seeks to identify bioactive molecules from wood decay fungal (WDF) extracts with activity against eukaryotic pathogens (especially Candida albicans and Plasmodium falciparum). The Saccharomyces cerevisiae model organism for eukaryotic cell biology studies is extensively used to screen bioactive molecul...
ABSTRACT Plasmodium falciparum gametocytes are able to infect the mosquito even at levels below microscopic detection and are responsible for malaria transmission. Gametocytes are exposed to immune pressure from the host and as a result, the host is able to develop antibodies against these gametocytes. However, it has not been determined whether naturally acquired immune response against gametocytes have a direct correlation with the presence of sub-microscopic gametocytes in the peripheral ...
ABSTRACT The suitability o f five Schistosoma genus-specific monoclonal antibodies (MoAbs) (Sh3/34.10, Sh3/38.2, Sh4/14.3, Sh5/32.30 and Sh5/34.10) in detecting schistosome antigens in infected human and cattle was evaluated. These antibodies were employed in various diagnostic assays to diagnose human and animal schistosomiasis. Extant MoAb secreting hybridoma cells were first propagated in culture to produce the MoAbs. The culture supernatants containing secreted antibodies were analysed b...
ABSTRACT Current efforts to eliminate malaria worldwide are soaring and one of the approaches to this is the mass screening of medicinal plants for their possible potential anti-malarial property. The current study conducted was to determine the effect of one such plant, Cryptolepis sanguinolenta on gametocyte development. In vitro growth inhibition assays on early and late gametocytes stage using ethanolic and aqueous extract of the C. sanguinolenta roots was performed. Enzyme activity assa...
ABSTRACT Human systemic fungal infections have increased in the past decade due to the dramatic increase in immunocompromised patients hence the emergence of resistant fungal strains. It is therefore necessary to discover new antifungal compounds with novel mode of action. This study was aimed at isolating and characterizing antifungal agents from wood decaying fungi (WDF). The growth conditions for maximum production of bioactive agents from WDF were first tested. It was observed that miner...