ABSTRACT The seed of Nigella sativa was evaluated for its chemical composition, medicinal as well as its nutritional values. The powdered seed was extracted with methanol to give the crude extract. Phytochemical screening of the crude extract revealed the presence of the following classes of natural products; alkaloids, tannins, flavonoids, saponins and anthraquinones, sterols and terpenes. The proximate analysis showed 12.00% moisture, ash content 3.00%, ether soluble extract 33.30% crude p...
ABSTRACT This study investigated the effect of ethanol leaf extract of Mucuna utilis (EEMu) on lead acetate induced toxicity on Wistar albino rats. Acute toxicity analysis of the plant extract produced no lethality even at high doses of 3000 mg/kg body weight. Twenty albino rats weighing 115 – 235 g were divided into four (4) groups; the first groupof animals served as normal control and were not administered either lead acetate or EEMU. The second group was treated intraperitoneally with ...
ABSTRACT This study assessed the phytochemical and nutrient compositions of edible yam species Dioscorea rotundata 1, Dioscorea rotundata 2, Dioscorea rotundata 3, Dioscuri rotundata 4, Dioscorea Cayenensis, Dioscorea alata, Dioscorea dumentorum and Dioscorea bulbifera in Southeastern Nigeria. The phytochemical and nutrient (proximate, minerals, vitamins, fatty acids and amino acids) compositions of the yam tubers were investigated using standard analytical methods.
ABSTRACT Eukaryotic pathogens are responsible for several serious human diseases, however, most drugs used as treatment regimens are ineffective while others still in use are toxic. This study seeks to identify bioactive molecules from wood decay fungal (WDF) extracts with activity against eukaryotic pathogens (especially Candida albicans and Plasmodium falciparum). The Saccharomyces cerevisiae model organism for eukaryotic cell biology studies is extensively used to screen bioactive molecul...
ABSTRACT Plasmodium falciparum gametocytes are able to infect the mosquito even at levels below microscopic detection and are responsible for malaria transmission. Gametocytes are exposed to immune pressure from the host and as a result, the host is able to develop antibodies against these gametocytes. However, it has not been determined whether naturally acquired immune response against gametocytes have a direct correlation with the presence of sub-microscopic gametocytes in the peripheral ...
ABSTRACT The suitability o f five Schistosoma genus-specific monoclonal antibodies (MoAbs) (Sh3/34.10, Sh3/38.2, Sh4/14.3, Sh5/32.30 and Sh5/34.10) in detecting schistosome antigens in infected human and cattle was evaluated. These antibodies were employed in various diagnostic assays to diagnose human and animal schistosomiasis. Extant MoAb secreting hybridoma cells were first propagated in culture to produce the MoAbs. The culture supernatants containing secreted antibodies were analysed b...
ABSTRACT Current efforts to eliminate malaria worldwide are soaring and one of the approaches to this is the mass screening of medicinal plants for their possible potential anti-malarial property. The current study conducted was to determine the effect of one such plant, Cryptolepis sanguinolenta on gametocyte development. In vitro growth inhibition assays on early and late gametocytes stage using ethanolic and aqueous extract of the C. sanguinolenta roots was performed. Enzyme activity assa...
ABSTRACT Human systemic fungal infections have increased in the past decade due to the dramatic increase in immunocompromised patients hence the emergence of resistant fungal strains. It is therefore necessary to discover new antifungal compounds with novel mode of action. This study was aimed at isolating and characterizing antifungal agents from wood decaying fungi (WDF). The growth conditions for maximum production of bioactive agents from WDF were first tested. It was observed that miner...
Urinalysis is the physical, chemical, and microscopic examination of urine. It involves a number of tests to detect and measure various compounds that pass through the urine. This presentation will give you an insight on everything you need to know about urine. Urine Composition Urinalysis Practical Experiments Urinalysis: (Normal & Abnormal Constituents of Urine) Estimation of pH of Different Samples Qualitative Tests on Lipids: Cholesterol �...
ABSTRACT The trade in traditional aphrodisiacs on the street of Nigeria is at all-time high. Several types are on display or advertised by vendors. One of such is Calotropis procera. The aphrodisiac potential of Calotropis procera aqueous stem extract was investigated on some reproductive markers in matured sexually active male albino rats. Phytochemical screening was performed following standard protocols, the mineral composition determination was also carried out using standard method. A to...
ABSTRACT Despite the frightening mortality rate associated with COVID-19, there is no known approved drug to effectively combat the pandemic. COVID-19 clinical manifestations include fever, fatigue, cough, shortness of breath, and other complications. At present, there is no known effective treatment or vaccine that can mitigate/inhibit SARSCoV-2. Available clinical intervention for COVID-19 is only palliative and limited to support. Thus, there is an exigent need for effective and non-invasi...
ABSTRACT The impact of pollution due to gas flaring on individuals native to Ebocha in Niger Delta Area was investigated. One hundred and five apparently healthy individuals between 21-70years who consented for the study were screened, seventy five individuals from Ebocha and thirty from Nsukka-a control location with no history of petroleum hydrocarbon pollution. Standard analytical procedures were used in the determination of the concentration of ascorbic acid (AA) of the individuals (male...
Aim: This study sought to evaluate the hepatoprotective potential of alkaloid extracts from V. doniana and F. thonningii leaves on alloxan-induced diabetic rats. Materials and Methods: Fresh leaves of V. doniana and F. thonningii were obtained from a local market Nkwagu in Abakaliki local government area of Ebonyi State. They were dried and pulverized to fine granules using manual grinder. Crude alkaloid was extracted using standard method. The acute oral toxicity of both plants was determine...
Abstract The aim of this study was to determine the ameliorative potential of Bambara nuts extract against acute ethanol-induced oxidative stress in Wistar rats. Bambara nuts of the Songkhla 1 variety locally sourced in Obinze area of Owerri, Imo State, Nigeria were powdered using a coffee grinder and an extract obtained from a Soxhlet apparatus with methanol as solvent. Twenty-four adult male Wistar rats were acclimatized for seven days and were randomly divided into four groups of six rats ...
Background: Bambara nut is a common household food in Nigeria especially among the Igbos ofthe South Eastern part of the country.Aim: This study is aimed at investigating the effect of Bambara nut on renal indices of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powder using a coffee grinder and extracted using soxhlet apparatus and methanol as the so...