Background: Bambara nut is a common household food in Nigeria especially among the Igbos ofthe South Eastern part of the country.Aim: This study is aimed at investigating the effect of Bambara nut on renal indices of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powder using a coffee grinder and extracted using soxhlet apparatus and methanol as the so...
Aim: This study sought to investigate the effect of Bambara nut on hepatic biomarkers of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powderusing a coffee grinder and extracted using soxhlet apparatus and methanol as the solvent. Twentyfour adult male Wistar rats were acclimatized for seven days during which they were fed ad libitum with standard fe...
Background: Xylopia aethiopica fruit has been reported to possess high medicinal value. Thus, people use it without any regard for its toxicity.Aim: This study is therefore aimed at investigating its toxicity on the liver of Wistar rats.Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria and were authenticated. They were air-dried and extracted using Soxhlet apparatus and ethanol as solvent. The median lethal dose (LD50) of the extract was d...
Abstract Gomphocarpus fruticosus is a wild plant that belongs to the Asclepiadaceae family and has many traditional uses. The plant has demonstrated by this study a significant level of microbial and phytochemical activity; the drug-resistant strains of microbes which have emerged in the last decade will be eradicated by the compounds this indigenous plant carries. This particular study aimed to scientifically validate the traditional uses of the plant G. fruticosus. The objectives of this st...
ABSTRACT The phytochemical contents of some milled Nigerian softwood chips were carried out in a quest to evaluate their potentials as sources of alternative medicine as well as uses in other industrial applications. The qualitative and quantitative analysis were ascertained. Tannin was found in all the Nigerian softwoods examined with the highest quantities obtained in Sterculia oblonga (1240 mg/100 g) and Barteria nigritiana (1230 mg/100 g). Highest quantities of alkaloid were obtained in C...
ABSTRACT The study aims to evaluate the effect of food chain mediated chronic exposure to arsenic and cadmium (singly and combined) on the level of reduced glutathione (GSH) and the activity of glutathione S-transferase (GST) in brains of rats. Fortymale Wistaralbino rats were fed with a diet containing fish fed with normal feed in water contaminated with 0.4 mg/100 ml cadmium (group B), arsenic (group C) and cadmium + arsenic (group D) respectively for three months. The control rats�...
ABSTRACT Aim: This study is aimed at assessing the effect of Xylopia aethiopica fruit on the lipid profile of Wistar rats. Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria and were authenticated. They were air-dried and extracted using Soxhlet apparatus and ethanol as solvent. The median lethal dose (LD50) of the extract was determined using standard met...
ABSTRACT Aim: This study is aimed at determining the effect of Xylopia aethiopica fruit on weight gain and blood sugar level of Wistar rats. Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria and were authenticated. They were air-dried and extracted using Soxhlet apparatus and ethanol as solvent. The median lethal dose (LD50) of the extract was determined using standard method. ...
ABSTRACT Background: The use of Xylopia aethiopica fruit in folklore medicine is on the increase without regard to its toxicity. Aim: This study was aimed at examining its toxicity on the kidney of Wistar rats. Methodology: The fruits of Xylopia aethiopica were air-dried and extracted using Soxhlet apparatus and ethanol as solvent. The median lethal dose (LD50) of the extract was determined using standard method....
INTRODUCTION: Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies ). This process starts by injecting a mouse (or other mammal) with an antigen that provokes an immune response. A type of white blood cell, the B cell, produces antibodies that bind to the injected antigen. These antibody producing B-cells are then harvested from the mouse and, in turn, fused with immortal B cell cancer cells, a myeloma , to produce a hy...
The aim of this study is to access the antihypertensivepotential of garlic juice in rats. To achieve this, 30 Wistar ratswere divided into three groups of 10 each. Rats in groups 1 and 2received aspecial prepared egg feed diet (24 egg yolkmixed with 1kg of normal rat diet) while those in group 3 received normalfeed diet and they served as the control group. Animals in group2 were administered 3 mL/100g body weight of undiluted garlicjuice simultaneously with the feeding. The animals were fedo...
Hypertension constitutes a major risk factor forseveral cardiovascular diseases such asatherosclerosis, heart failure, renal insufficiency,coronary artery disease and stroke. The risk factorincreases with age in both sexes. According to WHO,about one-third of the world’s population suffersfrom hypertension and the incidence has beenincreasing at a rapid rate due to life stylemodification. This study is aimed at investigating theeffect of egg yolk on blood pressure of Wistar rats. Toachieve ...
Aim: This study focuses on the effect of consumption of garri processed by traditional and instantmechanical methods on the lipid profile of Wistar rats.Methods: Cassava samples were obtained from the International Institute of Tropical Agriculture(IITA), Ibadan and were processed using traditional and instant mechanical methods. Fifteen adultmale Wistar rats were purchased from the Animal Holding Unit of the Department of Physiology,University of Ibadan, Nigeria with body weight between 100 ...
Recently, the use of ‘sunlight’ air freshener in Nigeria is on the increase. Some people use airfreshener in the office, car as well as in their homes, thereby constantly being exposed to it withoutconsideration of its adverse effect on human health. This study is therefore aimed at assessing theeffect of “sunlight” air freshener on oxidative stress biomarkers. Thirty (30) Wistar rats were dividedinto three groups of ten each and kept in different rooms. Rats in group 1 were not expos...
Background: The use of household insecticides for the eradication of insects especially mosquitoesin Nigeria is increasing. These insecticides are used without consideration of their adverse effect onhuman health.Aim: This study was aimed at accessing the effect of common household insecticides used inNigeria on male reproductive hormones of Wistar rats.Methodology: Thirty male Wistar rats were divided into five groups of six each and kept in differentrooms. Rats in group 1 were exposed to Ra...