Influence of Different Nitrogen Sources on Growth and Phb Production of Bacterial Isolates

ABSTRACT

Polyhydroxybutyrate (PHB) are biodegradable materials, which are accumulated to store carbon and energy in various microorganisms.                                   

Soil samples used for the study were collected from groundnut farm garden. The isolates were screened for PHB production using sudan III stain as well as submerged fermentation. Four (4) of the best PHB producing bacteria were selected for further study. A total of twenty one (21) bacteria were isolated form the soil sample in which Bacillus sp had the highest percentage frequency of occurrence (54.5%). Follow in order by Enterococcus sp  (13.63%), Veillonella sp  (9.1%),  Micrococcus sp. (4.5 %), Corynebacterium sp.(4.5%) Yersinia Pestis (4.5%), Lactobacillus fermenti (4.5%) and Citrobacter sp. (4.5%).

         The fermentation pattern of PHB production was studied using Nitrogen source. NH4Cl was found to be the best nitrogen source (1.560a) for growth of PHB producing bacteria  (Citrobacter sp) while maximum PHB production  (1.899a) was observed in Citrobacter sp when KNO3 was used as nitrogen source. The study carried out shown that nitrogen sources significantly influence the production of PHB .

TABLE OF CONTENT

Title page

Certification

Dedication

Acknowledgement

Abstract

Table of content

CHAPTER ONE

1.0    Introduction

1.1    Aims and objectives

1.2    Statement of problem

1.3    Justification

CHAPTER TWO

2.0    Literature review

CHAPTER THREE

3.0    Materials and method

3.1    Preparation of media

3.2    Isolation of bacterial from soil samples

3.3    Maintenance of culture

3.4    Identification of bacterial isolates

3.4.1 Morphological characterization

3.4.2 Biochemical characterization

3.5    Screening o isolate for PHB production using staining techniques.

3.6    PHB production

3.7    PHB extraction

CHAPTER FOUR

4.0    Result and discussion

CHAPTER FIVE

Conclusion and appendix