Isolation, identification and antibiotic resistance pattern of Pseudomonas species isolated from poultry feaces in owo metropolis

Pseudomonas species are most available pathogenic Gram negative bacteria in environmental samples. They are resistant to several drugs, opportunistic pathogen and their presence in the environment poses threat to the public health. The ability of microorganisms to survive in the presence of antimicrobial agents at a situation that can kill or enhance their growth is known as antimicrobial resistance. This has become a serious threat globally, which makes the treatment of infections in humans and animals expensive and difficult. This study aims at isolating, identifying and investigating the antibiotic resistance pattern of Pseudomonas species isolated from poultry feaces in Owo metropolis. Fresh feaces of 6 healthy boilers were collected separately into properly labeled and sterile capped bottles from two different privately owned poultry farms in Owo, Ondo state Nigeria. Pseudomonas species was isolated using Centrimide Agar, therefore, their morphological and cultural characteristics on MacConkey Agar, Nutrient Agar, and Eosin Methylene Blue Agar were equally observed, and the isolates were therefore characterized. Kirby Bauer disc (KBD) diffusion method was used for antibiogram of the isolates. The isolates presented different morphological and cultural characteristics on the saveral types of media used. At poultry farm A (43.3%) the total prevalence of the Pseudomonas species in the samples was low compared with (56.7%) at poultry farm B. The isolates indicated different resistant level to the antibiotics tested with resistance (3.3%) to the carbapenem (imipenem) and one of the tested cephem (cefotaxime) being the lowest and resistance (70.0%) to the nitrofuran (nitrofurantoin) being the highest; while 66.7% were multidrug resistant. This necessitates the implementation of mitigating strategies to limit the transfer of antibiotic-resistant Pseudomonas species from animals to humans.