Research Papers/Topics in Applied Microbiology

IN VITRO EVALUATION OF THE ANTIMICROBIAL POTENCY

 The search for potent antimicrobial agent is unending. The use of mouthwashes to improve oral hygiene is being favoured for its presumed antimicrobial activity. This study therefore evaluated the antibacterial activity of common mouthwashes against relevant clinical isolates.  Five mouthwash samples (coded; LIS; COL; TOT; FLU; BRE) bought from major super markets in Ondo State, Nigeria, were examined for antimicrobial activity using agar well diffusion method. The kinetic of activity and M...

Evaluation of the viability and survival rates of some selected pathogens

Artificially inoculated fruit juice is a very viable profit making sole business in Nigeria, the product is locally available in all market in different parts of the country, especially in Ondo state, using Ikare and Iwaro Akoko market, as the focal point of this present study. Fifty (50) samples of the inoculated fruit juice were purchase from the market in Iwaro and Ikare Akoko, Ondo State. The test organisms used for this research work are Staphylococcus aureus (ATCC) 25923, Salmonella spp...

Comparative Studies of the Efficiency of Sun Drying and Hot Air Oven

The drying efficacy and product quality of onion and pepper dried in the oven and sun were compared. The drying process took 1-4 days to accomplish. The proximate analysis, bacterial load and identification of microorganisms, sugar fermentation, catalyses test and the bacteria count were determined. The mean moisture content of the product at the end of the drying was 6.4% (sun) and 4.5% (oven). The oven dried product were significantly (P

Bioactivity and Phytochemical Screening of Nigerian Medicinal

The Aqueous, ethanol, hexane and ethyl acetate extracts of the leaves, bark and roots of Acacia albida, Anchomanes difformis, Boscia senegalensis, Bridelia ferruginea, Ficus ingens, Indigofera arrecta and Moringa oleifera were tested for possible source of antibacterial activities against selected clinical organisms found in the pediatrics hospital in Ondo and Ekiti State, Nigeria. The selected clinical organisms used for this research work are Salmonella typhi, Staphylococcus aureus, Klebsie...

Phytochemical Screening and Evaluation of Antioxidant and Proximate Properties of Morinda lucida Ethanolic Extract

Morinda lucida is a tropical West African evergreen shrub or small to medium sized rainforest tree, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. This study was designed to carry out the preliminary phytochemical screening and evaluation antioxidant and proximate properties of ethanol extracts prepared from the bark, leaves and root of M. lucida . It revealed the presence of saponin, tannins, a...

Phytochemical screening and antimicrobial properties of partially purified ethyl acetate

Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms used for the study are Escherichia coli, staphylococcus aureus, Candida albicans and Klebsiella pneumonia. The leaf and bark were extracted using one e...

Phytochemical Composition and Antimicrobial Activity of Daniella oliveri Extracts

Daniella oliveri (Caesalpiniacea) is a plant found in the Amazon region and other parts of South America and Africa. The tree may reach a height of 100 feet and trunk diameter of 4 feet. Daniella oliveri (Rolfe) Fabiaceae is commonly known as Ilorin balsam (eepo-iya) or Copaihu africana . It is an indigenous African tree, found extensively in Benin, Cameroon, Gambia and Nigeria. The basic objective of this project is to determine the antimicrobial activity and phytochemical composition of Dan...

Evaluation of Antibacterial Potency of Crude Ethyl Acetate and Ethanol Extracts of Morinda lucida

The plant Morinda lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in colour, flowers are white in colour, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The purpose of this work is to determine the antimicrobial properties of      Morinda lucida against Mycobacterium species, a very virulent and infectious organism, isolated ...

Elemental Composition, Evaluation of Anti-nutrients, and Antioxidant Potentials of Morinda lucida

Morinda lucida is a tropical West African tree of medium-size, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. The preliminary phytochemical screening of different extracts prepared from bark, leaves and seeds of M. lucida revealed the presence of Saponin, Tannins, Alkaloids, Flavonoids, and Cardiac glucosides. Furthermore, it also reveals presence of Minerals like Sodium, Potassium, Calcium, Iro...

Use of the Biomerieux id 32c Fungal Identification System for Fungal Isolated from Mother and Child Hospital Dump Site in Akure, Ondo State Nigeria

The purpose of this research work is to isolate and identify Using ID 32C API kit and to determine the antifungal sensitivity profile of fungi isolated from the mother and child dump site Akure, Ondo state, Nigeria.   Ten isolates (Yeast, Aspergiliusflavus, Aspergiliusniger, Fusariumsolani, Candida albucan, Candida kruise, Phytoptera, Rhizopusstolonifer, Trichodermhorizianum,Fusariumvortezelium) were used for this research work.Potato Dextrose Agar was used to isolate and prepared for enume...

Bio-prospective Screening of Antibacterial and Phytochemical Activity of Caesalpinia pulcherrima.Linn (Pride of Barbados) on Selected Clinical Isolates

This work was carried out to evaluate the antibacterial and Phytochemical activities of Caesalpiniapulcherrima  leaf and stem bark on some clinical isolates. The plant parts were collected from St. Benedict Catholic Church premises, Owena Barracks, Akure, Ondo State. Crude ethyl acetate extracts of Caesalpinia pulcherrima leaves and stem bark was assayed for its antibacterial activity against Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Bacillus subtilis, Escheric...

Prospects of Essential Oils in Drug Discovery

Abstract Essential oils (EO), according to the International Standard Organization (ISO)  defines essential oil as the product obtained from parts of plants by distillation with water steam and the product obtained by expression from pericarp of medicinal plant. The rapid growth and increase of the knowledge of identification, extraction, purification and characterization of essential oils in most local plant species is fast becoming an important aspect of the drug production process. ...

Evaluation of Antimicrobial Activity, Synergistic Efficacy, Qualitative and Quantitative Phytochemical Determination of Alstonia Boonei Leaf and Stem Bark on Selected Clinical Isolates

Abstract This study evaluated the antimicrobial activity, of Alstonia boonei leaf, stem bark and the synergistic efficacy of A. boonei leaf plus stem bark on seven bacteria (Gram positive and Gram negative bacteria) which are strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Proteus viridans, Salmonella typhi and Klebsiella pneumoniae, and three fungal isolates which are Candida albicans, Aspergillus flavus and Trichophyton rubrum. This study also ...

Evaluation of Antimicrobial Activity, Phytochemical Screening and Gas Chromatography-Mass/ Spectrophotometric Profile of Essential Oil from Persea americana & Citrus sinensis

Abstract This study aims to determine the phytochemical characteristics, structural determination of bioactive component from the leaf extract  using GCMs and experimentally investigation of the antibacterial activity of essential oil from Persea americana and Citrus sinensis leaf against seven bacteria (Gram-positive and Gram-negative) which are Staphylococcus aureus , Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia, Escherichia coli, Proteus mirabilis and Pseudomonasaeruginosa kn...

Efficacy of Essential Oils from Persea Americana stem bark and Seed extracts

 The aim of the study is to evaluate the Antibacterial, Phytochemical analysis of Essential Oil, From the Stem Bark and seed extracts of Persea americana (Avocado). This was carried out by the crude extraction of the seeds and stem bark extracts with n-hexane. The extracts were used to determine the presence of Phytochemicals. Stock cultures of test organism such as Staphylococcus aereus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Proteus mirabilis were...


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