Abstract: Fermentation is a metabolic process of microorganisms to obtain energy by breaking down organic compound. Potato peel waste was fermented with different concentration substrate (1gm, 2.5gm, 5gm, 10gm, 15gm, 20gm and 25gm) with 1% yeast and pH4.5 in different fermentation period (4th day, 8th day, 12th and 16 th days) with the aim of optimizing the substrate concentration for ethanol production. Among the different substrate concentration 10 gram of substrate produced more quantity ...
Abstract: Banana is an edible fruit and herbaceous flowering plant belonging to genus musa and family musaceae.Banana is a source of natural antioxidants and has good antimicrobial potential. Banana Peel is nutritional and rich in phytochemical compounds than its pulp. Characterization of the antioxidant and antimicrobial activities of endophytic fungi extracted from banana peel and pulp is the main purpose of this study.The banana fruit sample was collected from dingete locality Gemechis di...
Abstract: Fruit are very important for human being which contribute vital nutrients especially Vitamin C. However Fruit which are contaminated during harvesting and transporting. From different area. poor hygienic conditions, using of tap water for cleaning and unsafe environmental conditions of fruit resulting in different food borne illnesses. The study was aimed to assess bacteriological load of fruit served in cafes and restaurants of Chiro town. A cross sectional study was conducted fro...
Abstract: The effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate ...
Abstract: Monitoring of destructive invasive weeds such as those from the genus Striga requires accurate, near real-time predictions and integrated assessment techniques to enable better surveillance and consistent assessment initiatives. Thus, in this study, we predicted the potential ecological niche of Striga (Striga asiatica) weed in Zimbabwe, to identify and understand its propagation and map potentially vulnerable cropping areas. Vegetation phenology from remote sensing, bioclimatic an...
Escherichia coli which synthesize extended spectrum beta-lactamases (ESBL) have been implicated in severe human diseases. There is substantial evidence that cattle faeces have a role in developing and spread of multidrug-resistant pathogens, raising public health concerns. The study is aimed at detecting ESBL-producing E. coli associated with cattle faeces within Owo metropolis. Freshly passed faecal samples were taken aseptically from 9 apparently healthy cattle that were about to be sl...
ABSTRACT Microbiological and physico-chemical analyses and sensory evaluation were carried out on commercially and freshly prepared orange juice (100%) in the laboratory and its shelf- life after the storage period of 90 days using various storage methods. At the end of the study, the result showed that the laboratory processed orange juice in terms of the nutritional composition when compared with the commercially processed orange juice had a better quality considering the parameters assess...
The emergence of new drugs is often driven by the escalating resistance of parasites to existing drugs and the accessibility of more advanced technology platforms. Microfluidic devices have allowed for quicker testing of compounds, regulated sampling/sorting of entire animals, and automated behavioral pattern detection. In the majority of microfluidic devices, the effects of drugs on small animals (e.g. Caenorhabditis elegans)elegant are quantified by an endpoint, dose response curve that sho...
This new microfluidic platform offers a more sensitive method for screening drugs and their effects on the locomotion of free living and parasitic nematodes. The chip is capable of monitoring four nematode parameters (number of worms responsive, number of worms leaving the drug well, average worm velocity and time until unresponsiveness) to provide a more detailed analysis and resolution of drug resistance. The drug screening experiment is divided into three parts: pre-exposure study, dose ex...
An open microfluidic system has been developed to dispense and manipulate individual droplets on plastic sheets. Superhydrophobic material is applied to the surface of obtainable plastic sheets by spraying, then hydrophilic symbols are printed with an inkjet printer. The plastic sheets are connected to a two-axis tilting platform driven by stepper motors, which produces mechanical agitation enabling droplet transport. The system has been demonstrated to carry out various droplet operations, s...
ABSTRACT Broiler chicken meat is now considered as a major fast-growing source of meat in many parts of Tanzania especially Dar es salaam. The consumers demand safe and hygienic products without contamination with pathogenic microorganisms when the production and consumption of broiler chicken meat is gradually increasing. The present study was conducted to assess the bacterial contamination of broiler chicken meat collected from different areas around Dar es salaam especially Temeke and...
ABSTRACT This project is basically concerned with the extraction of DNA in tomatoes and garden egg fruit. An experiment on how to extract DNA on tomatoes and garden egg, isolation on it to see what DNA actually look like as well as importance of DNA extraction are consid3ered in this project. TABLE OF CONTENT Title page ……………………………………………………… i. Certification……………………………………………………..ii Dedication………………...
Abstract The antimicrobial resistance or otherwise of Coagulase-negative Staphylococci (CoNS) isolates from medical students of some South Eastern Nigerian Universities were determined. Swab samples were obtained from the students and were cultured for CoNS using standard microbiological methods. Isolates obtained were assayed for antibiotic resistance using disc diffusion method. MecA and blaZ genes on 533bp and 510bp respectively, of the isolates were amplify using the Polymerase Chain Reac...
ABSTRACTNigeria has energy crisis, hence, there is urgent need to diversify the country’s energy resources towards renewable alternatives for economic and social transformation. Biogas generated from the anaerobic digestion of local biomass resources, will increase national energy security as well as reduce dependence on fossil fuels. Optimization of methane production by codigestion of poultry feather and sawdust using microbial consortia were investigated. The results of the molecular ide...
Abstract: The negative impacts of hydrocarbon pollutants on our environments and economic resources cannot be overemphasized. The need to harness the bioremediation potentials of microorganisms associated with hydrocarbon pollution becomes very imperative in order to mitigate the environmental impacts of petroleum hydrocarbons in our environments. The molecular identification of the microorganisms isolated from four automobile workshops at Ugbene 2 Abakpa Nike, Enugu, Nigeria based on 1...