Research Papers/Topics in Biochemistry

In vitro antioxidant activity and nitric oxide activity on citrullus lanatus seeds

Citrullus lanatus (water melon) is a commonly consumed fruit worldwide. The seeds are often thrown away without considering its potential nutritional and medicinal benefits. In this study, a methanol extract of citrullus lanatus seeds was analyzed for the presence of bioactive secondary metabolites and to determine its ability to scavenge 2, 2‐ diphenyl ‐1‐ picrylhydrazyl (DPPH.) radical, superoxide anion radical (O2.‐), and nitric oxide radical (NO.). Quantitative phytochemical a...

Purification and characterization of rhodanese from sprouted maize

Rhodanese, a cyanide detoxifying enzyme, was isolated and partially purified from malted maize (Zea mays) using 85% ammonium sulphate precipitation and ion-exchange chromatography on CM-Sephadex C-25. The specific activity of the enzyme obtained was 5.07RU/mg protein with a yield of 9.6% and purification fold of 2.13. The Michaelis constant (Km) values of the substrates sodium thiolsulphate (Na2S2O3) and potassium cyanide (KCN) were 80 mM and 300mM while their Vmax were 4.86 RU/ml/min and...

EFFECT OF ISONIAZID ON THE METABOLISM OF PARACETAMOL IN RATS

ABSTRACTThe metabolism of Paracetamol has been studied in Isoniazid pretreated albino rats and comparedwith control. During this study, thirty-five (35) laboratory albino rats were used divided into five (5)groups of seven (7) rats each. Group I served as control and the rats were only administered with120mg/5ml/kg body weight dose of Paracetamol interperitoneally. However, Groups II-V servedas test groups and the members were pretreated with 10mg/kg body weight dose of Isoniazidinterperitone...

Technical Report on Students Industrial Work Experience Scheme

Students industrial work experience scheme SIWES is a skill learning programme designed to expose and prepare students of engineering, technology, environmental sciences, medical sciences, pure and applied sciences for the industrial work situation which they are likely to meet after graduation. Duration for SIWES is a six months programme for universities, four months for polytechnics end of ND 1, four months for colleges of Education end of NCE II. The students industrial work experience s...

REVIEW: BETA LACTAM ANTIBIOTIC DRUG RESISTANCE

The class of β-lactam antibiotics has  antibiotic agents that possess β-lactam ring as part of their molecular structure, these agents includes cephalosporins, monobactams, penicillins   and carbapenems. these are the most widely used antibiotics which act by inhibiting the synthesis of the bacterial cell wall such activity leads to the lysis and death of the bacteria. due to the wide applications of these antibiotics bacteria have developed resistance mechanism against these...

Goitrogenic content of some selected nigerian leafy vegetables

The goitrogenic potential of a plant or food depends upon the amount of active goitrogen present in it. Various procedures like soaking, washing, boiling and cooking can help in reducing the goitrogenic potency of these foods. These, along with the intake of iodide supplements are generally practiced in areas where goitrogenic foods are routinely consumed. In this experiment, goitrogenic contents of some commonly consumed vegetables were assayed and analysed. 

Ethanolic extract of Jatropha gossypifolia exacerbates Potassium Bromate-induced clastogenicity, hepatotoxicity, and lipid peroxidation in rats

Extracts  of  J.  gossypifolia  L.  have  been  reported  to  have  several  medicinal  values,  including potential  anti-cancer  and  anti-inflammatory  properties.  In  this  study,  we  investigated  the  anti-clastogenic and  hepatoprotective,  effects  of  the  ethanolic  leaf  extract  of  J.  gossypifolia  L.&nbs...

Brain, lung, and heart oxidative stress assessment of an overthe-counter pyrethroid insecticide product in Nigeria

We  evaluated  the  brain,  lung,  and  heart  oxidative  stress  in  rats  exposed  to  aerosol  of  an  over-thecounter  pyrethroid  insecticide  product  in  Nigeria.  The  experimental  animals  were  randomly  divided  into  four  groups: group  I  (control)  was  not  exposed  to  the  insecticide&nb...

Potential Genotoxicity, Hematotoxicity and Clastogenicity of Three Commonly Consumed Polyherbal Formulations in Abeokuta South-West Nigeria

Background:  The  current  upsurge  in  the  use  of  polyherbal  remedies  and  coupled  with  loose  regulation  on  public access  to  these  products  underscore  research  efforts  to  evaluate  their  biochemical  effect,  noting  also  that  many  of  the polyherbal  medicines  lack  scientific  evidence...

Extra-pulmonary oxidative stress investigations of an over-the-counter pyrethroid insecticide product in rats

Extra-pulmonary  oxidative  stress  investigations  of  exposure  to  aerosol,  of  an  over-the-counter pyrethroid  insecticide  product  in  Nigeria  in  Wistar  rats  were  carried  out.  Four  groups  of  five  animals each  were  used  in  this  study,  and  were  exposed  to  different  concentrations  of...

Edible camphor-induced histopathological changes in hippocampus and cerebral cortex following oral administration into rats

Introduction:  Raw edible camphor (EC), and as component of herbal infusions  are widely used to treat pile, back pain, erectile dysfunction, and as  an aphrodisiac especially in preparation  for sexual intercourse by men. It has been traced in umbilical cord, blood,  fetal, adipose,  and other tissues including brain, where it bioaccumulates.  Methods:  The study, therefore, investigated the possible histopathological changes  in brain, heart, and  spleen that may result following ...

Induction of micronuclei in bone marrow cells and hepatotoxicity of one of the most common over-the-counter pyrethroid insecticide products in Nigeria

Increasing environmental pollution in the tropics is creating a breeding ground for mosquitoes, leading to increasingly frequent use of insecticides to combat home infestation of mosquitoes, the vector of malarial parasites. Household members are therefore more prone to aerosol exposure and subsequent health effects. We assessed the hepatotoxic and clastogenic effects in rats exposed to different levels of aerosols of one of the insecticides most commonly in used in Nigeria. There were signif...

ANTICLASTOGENIC AND HEPATOPROTECTIVE PROPERTIES OF GINGER (ZINGIBER OFFICINALE) EXTRACT AGAINST NITROBENZENE-INDUCED TOXICITY IN RATS

Ginger  or  Zingiber  officinale  has  been  reported  to  possess  antioxidant  activities  that  can protect  against  free  radical  damage.  We  therefore  carried  out  this  study  to  investigate  the  possible ameliorative  effects  of  ginger  against  the  clastogenicity,  hepatotoxicity  and  oxidative  s...

ROLE OF CIRCADIAN CLOCK GENES IN THE REGULATION OF CELL CYCLE PROCESSES

One  interesting  branch  of  biochemistry  which  scientists  are  still  trying  to completely  unravel is the  area  of  chronobiochemistry.  The  history  of  chronobiology  is  dated  400  B.C.,  when  Androsthenes observes  that  leaves  of  certain  trees  open  during  the  day  and  close  at  night.&n...

Effect of edible camphor administrations on levels of steroid and thyroid hormones in male wistar rats_content_1

ABSTRACT Background:  Edible  camphor  (EC)  is  one  of  the  ingredients  in  the  preparation  of  local infusion  for  the  management  and  treatment  of  pile,  back  pain,  and  erectile  dysfunction, which  are  practices  commonly  in  the  south-western  part  of  Nigeria.  Methods:  Thirty  rats  were  used  for  the  study,  and  were  divided  into  six  groups  of  five rats  each.  Group  I  animals  served  as  normal  ...

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