Plasmid Profile Of Streptococcus lactis And Lactobacillus Plantarum Isolated From Ogi Encoding For Acetaldehyde In Yoghurt

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Abstract

An investigation was carried out on the plasmid profile of Streptococcus lactis and Lactobacillus plantanum isolated from a total of 120 samples collected from Oyingbo, a local market in Lagos State. Morphological, cultural, physiological characterization and API kits were employed to isolate and identify and treptococcus lactis and Lactobacillus plantanum. All the isolates harboured plasmids ranging from 1-7 in numbers and 3kb – 21.5 kb in sizes. Distinct plasmid patterns were seen using the electrophoresis of plasmid DNA method. In this study, single strains and mixed cultures of the isolates were investigated for acetaldehyde production in yoghurt (fermented milk product) with the isolates using column trapping gas liquid chromatography technique. The results show that acetaldehyde production of the isolates were somewhat variable from strain to strain and hence could be used as starter cultures for yoghurt production with market acceptability.

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