Preventive and Therapeutic Activities of Methanolic Extract of Talinum triangulare Leaves against Ethanol-Induced Oxidative Stress in Wistar Rats

Preventive and therapeutic activities of methanolic
extract of Talinum triangulare leaves against
ethanol-induced oxidative stress in wistar rats is the
aim of this study. Fresh plants of T. triangulare were
purchased at a local market in Orita-Challenge,
Ibadan. The leaves were dried and extracted using
soxhlet apparatus and methanol as the solvent. The
methanol was evaporated in a rotary evaporator at
35 oC with a yield of 2.76 g which represents a
percentage yield of 11.04%. Twenty Wistar rats with
body weight between 120 and 150 g were used for
this study. They were randomly divided into four
groups of five rats each. Animals in groups A and B
were administered saline solution while those in
groups C and D were administered T. triangulare
extract for twenty-one days. The animals were
administered the extract and saline solution at a dose
of 4 mL per 100 g body weight 12 hourly via oral
route of administration. At the end of the treatment,
they were fasted overnight and animals in groups B
and D were exposed to a single dose of 70% ethanol
at 12 ml/kg body weight to induce oxidative stress.
After 12 hours of ethanol administration, the animals
were anaesthetized using diethyl ether and were
sacrificed. Liver was excised, weighed and
homogenized in 50 mmol/L Tris–HCl buffer (pH 7.4)
and then centrifuged at 5000 × g for 15 minutes for
biochemical analysis. Supernatants were immediately
kept frozen for further analysis. Ethanol-induced
oxidative stress significantly increased the activities
of AST, ALT, LDH, LPO, CAT, SOD and GPX but
decreased GSH. These effects were regulated by T.
triangulare administration. T. triangulare was able
to remedy the effect of ethanol by regulating the
oxidative stress biomarkers, thus possesses
preventive and therapeutic potency against ethanolinduced oxidative stress and can protect the body
against free radicals arising from oxidative stress.