CHARACTERISATION OF ESSENTIAL OIL FROM LEAVES OF Euphorbia heterophylla AND ITS INSECT REPELLENT POTENTIAL

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This study aimed at characterizing essential oil from leaves of E.heterophylla and its insect repellent potential. Essential oil from fresh leaves of E.heterophylla was extracted by hydro distillation method, using a Clevenger apparatus for 4 hours and the yield was 0.1816%w/w. Repellence test was conducted by using 5 cockroaches in number which were placed in a modified Y-tube Olfactometer and observations recorded for each and every odour introduced after 5 minutes in a relatively dark room. Repellency activity was computed in percentage form and it showed 100%. Analysis of the chemical constituents of the essential oil was done by Gas Chromatography coupled with a Mass Selective Detector, Ethyl benzene(4.58%), p-Xylene (2.22%),o-Xylene(2.73%), Nonane(1.70%), Benzene,1-ethyl-3-methyl-(1.09%), Benzene ,1,3,5trimethyl(1.09%),Decane (0.82%), Limonene (3.39%), Undecane (0.44%), 3-cyclohexen-1-ol, 4methyl-1-(1-methylethyl)-(0.37%) and Dodecane (6.07%) as the constituents of the essential oil from E.heterophylla leaves. All these chemical constituents are active ingredients for insect repellants, thus this essential oil can be used a natural based insect repellant t replace the synthetic insect repellants.

Key words: Essential oil, Insect repellent potential, Insect repellant, DEET, GC-MS and Euphorbia heterophylla.

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CHARACTERISATION OF ESSENTIAL OIL FROM LEAVES OF Euphorbia heterophylla AND ITS INSECT REPELLENT POTENTIAL

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