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Laboratory Investigations on the Potential of Entomopathogenic Fungi for Biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) Larvae and Pupae

Abstract: The susceptibility of third instar Helicoverpa armigera to seven strains of three entomopathogenic fungal species, i.e. Metarhizium anisopliae, Beauveria bassiana and Paecilomyces fumosoroseus , was tested under laboratory conditions using the larval immersion method. High efficacies ranging from 68 to 100% corrected mortality were recorded with more profound effects in treatments with B. bassiana and P. fumosoroseus strains. The median lethal concentration (LC50) for L3 was 6.0 10...

Comparative Analysis And Prediction Of Remediation Rates Of Microbes In Simulated Polluted Soil Samples

ABSTRACT    The study compared and predicted the remediation rates of microbes using the rate removal of Total Petroleum Hydrocarbon Content (TPHC) of a crude oil polluted land. This  was done using field experimental data generated from the soil of the Federal University of Technology Owerri Research Farm in Imo State, Nigeria. The soil was artificially polluted in the laboratory with crude oil - Bonny light - with specific gravity of 0.8323. Petroleum contaminant present in the soil was ...

Comparative Analysis And Prediction Of Remediation Rates Of Microbes In Simulated Polluted Soil Samples

ABSTRACT The study compared and predicted the remediation rates of microbes using the rate removal of Total Petroleum Hydrocarbon Content (TPHC) of a crude oil polluted land. This was done using field experimental data generated from the soil of the Federal University of Technology Owerri Research Farm in Imo State, Nigeria. The soil was artificially polluted in the laboratory with crude oil - Bonny light - with specific gravity of 0.8323. Petroleum contaminant present in the soil was 230mg ...

Rapid Bioconversion Of Rice Straw Using Cellulolytic Cultures For Improved And Sustainable Crop Productivity And Soil Fertility In Mwea, Kenya

ABSTRACT  Proper management strategies for huge amounts of crop residue generated from agricultural farms need to be obtained. Composting is a good strategy in rice straw management. Traditional methods of composting rice straw are limited in timely production of good quality compost due to its recalcitrant nature. There is need to develop and formulate microbial starter cultures that are adapted to local climatic conditions to ensure efficiency in using microorganisms in composting. This st...

STUDIES ON THE MYCOFLORA AND SOME PHYSICAL CHARACTERISTICS OF GHANAIAN MAIZE (Zea mays L) VARIETIES AND THE EFFECT OF EXTRACTS OF ZANTHOXYLUM XANTHOXYLOIDES LAM AND KIGELIA AFRICANA BENTH ON

ABSTRACT Mycoflora of mixed maize grains and the newly developed Abeleehi and Obaatanpa varieties and airspora in the Warehouse o f the Ghana Food Distribution, GFDC, at Balduzzi, Kumasi have been studied under normal and simulated ambient Environmental Relative Humidities (ERH ’s) representative of the Ghanaian conditions. Some important physical and processing characteristics of £. and normal stackbumed white and yellow maize (seed water absorption, swelling index, 1000-seed weight, ferm...

Effects of Botanicals and Biocontrol Agents on Growth and Aflatoxin Production by Aspergillus Flavus Infecting Maize in Some Parts of Nigeria

ABSTRACT In a comprehensive study to assess the effects of botanicals and biocontrol agents on growth and aflatoxin (AF) production by Aspergillus flavus (A. flavus) infecting maize, a total of 1143 maize samples, collected in eighteen batches of five maize varieties (yellow, white, pop, variegated and mixed) from northern and southern parts of Nigeria were investigated between June 2011 to December 2013. Samples collected from field, 414 (36.2%) and stored batches, 729 (63.8%) were cultured...

Enumeration of Most Common Microbes Responsible for Tomatoes Spoilage

Abstract This study investigated the microorganisms associated with the spoilage of tomato (Lycopersicum esculentum) obtained from two markets in Jahun town, Jahun Local Government in Jigawa State, Nigeria. A total of five species of bacteria were isolated and identified viz: Bacillus subtilis, B.aureus, Escherichia coli, Klebsiella aerogenes and Staphylococcus aureus. The most prevalent bacterial isolate was Bacillus subtilis with 24% and was found in all samples from the two markets. Klebsi...

Effects of seedling age on colonization patterns of Citrus limon plants by endophytic Beauveria bassiana and Metarhizium anisopliae and their influence on seedlings growth.

Abstract: The inoculation methods, the fungal strains, and several other factors are known to influent the success of fungal entomopathogens colonization in plants. The physiological status of the plant could also be another determinant. In the present study, the ability of three strains of Beauveria bassiana and one strain of Metarhizium anisopliae to successfully colonize Citrus limon plants and the influence of seedling age on endophytic colonization success was examined. Three, 4, and 6 ...

Isolation and characterization of Streptomyces species with antifungal activity from selected national parks in Kenya

Abstract: Microorganisms and their natural products are potentially important for the biological control of crop diseases without detrimental effects to the environment. In this study, acetonitrile-methanol extracts of 361 actinobacterial isolates obtained from Aberdares, Arabuko Sokoke, Lake Bogoria, Mt Kenya,Kakamega, Ruma, Shimba Hills and Imenti forest national parks in Kenya were screened for antagonism against Fusarium oxysporum, Fusarium spp and Colletotrichum kahawae, which are impor...

Post-Harvest Fungi Diversity And Level Of Aflatoxin Contamination In Stored Maize: Cases Of Kitui, Nakuru And Trans-Nzoia Counties In Kenya

ABSTRACT Aflatoxin contamination of maize in Africa poses a major threat to food security and the health of many African people. In Kenya, aflatoxin contamination of maize is high due to the environmental, agricultural and socio-economic factors. Many studies have been conducted to understand the scope of the problem especially at pre-harvest level, but few of them have been concentrated to the post-harvest level. This research was carried out to gather scientific information on the fungi pop...

Performance Characteristics Of Fungal Degraded Rice Bran And Palm Kernel Cake Substituted In Clarias Gariepinus Feeds

ABSTRACT Fish farming enterprise has increased in recent times due to public demand for fish protein. However, rising costs of fish feed ingredients have greatly reduced the profit margin of local fish farmers. Cheap, readily available and locally sourced ingredients may be better substitutes to the costly fish feeds. The aim of this study was to evaluate the performance of fermented Palm Kernel Cake (PKC) and Rice Bran (RB) as substitutes in the diet of Clarias gariepinus. Fungi were isolate...

Development of formulations and delivery systems to control economically important ticks with entomopathogenic fungi

Abstract: Due to the rapid development of tick resistance to synthetic chemical acaricides and the potential risk posed by these chemicals to non-target species, efforts are intensifying towards the development of entomopathogenic fungi as an environmentally benign alternative tick control strategy. The main objective of this study is the development of formulations and delivery systems for control of ticks with entomopathogenic fungi. Screening of entomopathogenic fungal isolates is the fir...

Diversity And Antibiogram of Microbial Contaminants Isolated From Foods Hawked in Nsukka Metropolis : Consideration For Public Health

ABSTRACT Hawked foods are cheap and easily accessible and have met the food needs of people in many cities and towns of developing countries. This study was aimed at ascertaining the diversity of microbial contaminants in foods hawked in Nsukka town. Seven different food samples of cooked foods hawked in Nsukka were examined for the presence of bacterial and fungal contaminants using the streak plate method on sterile nutrient agar plates and SDA plates respectively. The foods included; pige...

Isolation, Identification And Characterization Of Microbial Contaminants In Selected Biosafety Laboratories In Kenya

ABSTRACT Microbial contamination in laboratories and hospitals is becoming serious problem worldwide and characterization of such contaminants offer hope for treating some hospital and laboratory acquired infections (LAI). Microbial contaminants on benches, floors, media and in equipment can be influenced by temperature, humidity, nutrient media used in the laboratories as well as storage conditions of the media. Therefore there is need to determine the microbial sources, isolate and identify...

Evaluation of the phytochemical composition and antimicrobial activity of lemongrass (Cymbopogon citratus) against some human pathogenic microorganisms

ABSTRACT Antibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus (lemon grass) against selected microbial pathogens using chloroform and methanol as extracting solvents were assessed. The minimum inhibitory, bacteri...


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