By utilizing a low-cost engineering tool, we have created a microfluidic platform to study bacteria at the single cell level, allowing us to unlock insights into microbial physiology and genetics that would otherwise not be possible. The platform is composed of 3D devices made of adhesive tapes, an agarose membrane as the resting substrate, a temperature-controlled environmental chamber, and an autofocusing module. With this technology, we have been able to observe Escherichia coli morphologi...
In this paper, we explore the use of microfluidic paper-based analytical devices (lPADs) to study the behavior of Caenorhabditis elegans. We show how these devices can be fabricated on paper and plastic substrates, as well as how to load, visualize, and transfer single and multiple nematodes. We also demonstrate the use of anthelmintic drug, levamisole, to perform chemical testing on C. elegans. Furthermore, we provide a custom program that is able to recognize individual worms on the lPADs i...
The effects of the workplace on the safety, health, and productivity of personnel can be seen at various levels. To protect and boost general worker health, innovative hardware and software tools have been developed for the detection, elimination, substitution, and regulation of occupational hazards. Wearable technologies make it possible for constant tracking of workers and their environment, whereas connected worker solutions provide contextual information and support for decision-making. H...
The effects of the workplace on the safety, health, and productivity of personnel can be seen at various levels. To protect and boost general worker health, innovative hardware and software tools have been developed for the detection, elimination, substitution, and regulation of occupational hazards. Wearable technologies make it possible for constant tracking of workers and their environment, whereas connected worker solutions provide contextual information and support for decision-making. H...
Bacterial populations grown in a lab environment are typically seen as homogeneous in terms of growth rate, size, and content. However, recent findings have shown that even during exponential growth phase, these cells can be heterogeneous. This has important consequences for how bacteria respond to stressors such as antibiotics. For example, the concept of antimicrobial persistence is linked to a small subpopulation of cells that enter a dormant state where antibiotics are no longer effective...
In this review, we focus on the hardware and software technologies used for the purpose of gastrointestinal tract monitoring in a safe and comfortable manner. We review the FDA guidelines for ingestible wireless telemetric medical devices, and the features incorporated in capsule systems such as microrobotics, closed-loop feedback, physiological sensing, nerve stimulation, sampling and delivery, panoramic imaging and rapid reading software. Both experimental and commercialized capsule systems...
We propose a remote monitoring device for measuring behavioral indicators like posture, gait, vocalization, and external temperature which can help in evaluating the health and welfare of pigs. The multiparameter electronic sensor board was tested in a laboratory and on animals. Machine learning algorithms and decision support tools can be used to detect lameness, lethargy, pain, injury, and distress. The roadmap for technology adoption, potential benefits, and further challenges are discusse...
This paper presents a remote monitoring tool for the objective measurement of behavioral indicators that can help in assessing the health and welfare of pigs in precision swine production. The multiparameter electronic sensor board can measure posture, gait, vocalization, and external temperature, and has been characterized through laboratory measurements and animal tests. Machine learning algorithms and decision support tools can be implemented to detect animal lameness, lethargy, pain, inju...
Electronic sensors to monitor pig behavior in farms.
We present the NERV, a nonmechanical, unidirectional valve, to control the locomotion of Caenorhabditis elegant (C. elegans) in microfluidic devices. This valve is created by establishing a region of lateral electric field which can be toggled between on and off states. We observed that C. elegans do not prefer to advance into this region when the field lines are facing their movement, so when they reach the boundary of the NERV, they partway enter the field, retreat, and switch direction. We...
It is becoming more essential to identify and recognize the phenotypes of anti-parasitic drug-resistant isolates. Current molecular methods of doing so are restricted. In this paper, we showcase a microfluidic bioassay to measure phenotype using parameters of nematode locomotion, using larvae of the animal parasite Oesophagostomum dentatum. Parameters of sinusoidal movement, including propagation speed, wavelength, wave amplitude, and oscillation frequency, were dependent on the levamisole-se...
ABSTRACT The Conventional Levenspiel and Smith fixed point continuous monitoring method is used in the evaluation of the dispersion number and the dispersion coefficient, I? of river Ebm under study. Comparisons were made with Agunwamba’s method and both methods for different results. The leverspiel and Smith method gave higher values than those obtained with the Agunwamba's method except in me station. This is due to experimental errors. The Agunwamba's method though more tedious gave mor...
ABSTRACT The objective of this research is to investigate the effect of addition of carbide on sewage degradation. To provide answers to the above objective, some laboratory tests were conducted which include Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total coliform, pH and Suspended Solids (SS) tests. Six experimental set ups of 5liters of sewage were constructed in the laboratory under anaerobic condition. Each of the sewage contained 0g (which served as control), 1g, ...
This research work, which presents a simplified technique of modelling steel fibers, uses python algorithm that can be directly run in ABAQUS to automatically generate the location and orientation of steel fibers in a rectangular ultra-high performance concrete specimen, so that the effect of steel fibers on the properties of such a specimen can be directly investigated through numerical simulation. This method is fast, can be applied to structural members of bigger dimensions (like beams,...
Linear analysis mostly applies to the problems that satisfy linear assumptions, materially linear problems that will have less displacements, less deformations, and boundary conditions which is constant. If any one of the above assumptions is not satisfied, a nonlinear analysis will have to be performed. ADINA Structures will provide powerful solving capacity and formulations which allows the solution of extremely complicated models with small CPU time and other memory requirements.