Abstract: Edible insects are increasingly gaining popularity as research reveals multiple benefits. However, the rediscovery of natural products from insects as medicinal agents has received limited attention. This study aimed at evaluating the diversity of sterols in extracts of nine edible insects and potential antibacterial activities. Dichloromethane extracts of these insects were analyzed using gas chromatography–mass spectrometry to identify important sterols, followed by evaluation ...
Abstract: Reliable metrics to monitor human impacts on biodiversity are essential for informing conservation policy. As insects are indicators of global change, whose declines profoundly affect ecosystems, insect diversity may predict biodiversity status. Here we present an unbiased and straightforward biodiversity status metric based on insect diversity (richness) and landscape naturalness. Insect diversity was estimated using spatially explicit earth observation data and insect species ass...
Abstract: Analysis of landmark-based morphometric measurements taken on body parts of insects have been a useful taxonomic approach alongside DNA barcoding in insect identification. Statistical analysis of morphometrics have largely been dominated by traditional methods and approaches such as principal component analysis (PCA), canonical variate analysis (CVA) and discriminant analysis (DA). However, advancement in computing power creates a paradigm shift to apply modern tools such as machin...
Abstract: The Anopheles symbiont, Microsporidia MB, is maternally inherited and has a strong malaria transmission-blocking phenotype in Anopheles arabiensis. Microsporidia MB is also vertically transmitted, sexually transmitted, and avirulent. These characteristics are expected to promote its spread through mosquito populations, enhancing the potential of Microsporidia MB as a candidate for the development of a symbiont-mediated malaria transmission-blocking strategy. We found that the patte...
Abstract: The utilization of insect protein in poultry feed is globally gaining momentum. However, the nutritional quality of meat from hen fed diet with black soldier fly larvae meal (BSFLM) as fishmeal (FM) substitute has received limited research attention. Our results revealed that feed substitution did not affect the proximate compositions of the meat products. Omega 3 fatty acids were uninfluenced (P < 0.05) whilst the total monounsaturated fatty acids progressively increased with incr...
Abstract: Background: PermaNet® 3.0 is a deltamethrin-treated combination long-lasting insecticidal net with the addition of synergist piperonylbutoxide (PBO) on its roof section. It is designed to overcome the challenge posed by pyrethroid resistant vector populations against mainstream long-lasting insecticidal nets impregnated with pyrethroids only. The objective of this study was to determine insecticide resistance status of Anopheline and Culicine mosquitoes, to evaluate the bio-efcacy...
Abstract: : This study was carried out to assess individuals’ willingness to pay (WTP) for UZIMAX,a novel plant-based biopesticide developed for malaria vector control. The biopesticide is estimated to kill up to 100% of Anopheles larvae within 48 h of application and poses no risks to human health and the environment. However, scaling-up of its adoption requires clear evidence of its acceptance by individuals in malaria-prone areas. We conducted Becker-DeGroot-Marschak (BDM) revealed pref...
Abstract: Background: In Kenya, malaria remains a major public health menace equally affecting the semi-arid to arid ecologies. However, entomologic knowledge of malaria vectors in such areas remains poor.Methods: Morphologically-identified wild-caught Anopheles funestus (s.l.) specimens trapped outdoors from the semi-arid to arid area of Kacheliba, West Pokot County, Kenya, were analysed by PCR and sequencing for species identification, malaria parasite infection and host blood-meal sources...
Abstract: Background: The search for a standard human surrogate in the form of a synthetic mosquito attractant has been the goal of many laboratories around the world. Besides alleviating the occupational risk subjected to volunteers participating in vector surveillance and control, discovery of potent attractants underpins the development and deployment of mass trapping devices for controlling mosquito-borne diseases. Methods: A dual-port olfactometer was used to assess behavioural response...
Abstract: Background & objectives: A study was conducted to characterise larval habitats and to determine spatial heterogeneity of the Anopheles mosquito larvae. The study was conducted from May to June 1999 in nine villages along the Kenyan coast. Methods: Aquatic habitats were sampled by use of standard dipping technique. The habitats were characterised based on size, pH, distance to the nearest house, coverage of canopy, surface debris, algae and emergent plants, turbidity, substrate, and...
Abstract: What does ‘mosquito specialist’mean? A mosquito specialist is a predator that actively prefers mosquitoes, by which we mean that it is differentially motivated to capture mosquitoes among possible prey. This would make it extraordinarily useful, as it would be targeting an insect that matters to people. At best, the mosquito is a nuisance and, at worst,it is a notorious disease vector. There may be many predators that eat alot of mosquitoes, but experimental evidence is needed ...
Abstract: Novel insect-specific viruses (ISVs) are being discovered in many important vectors due to advances in sequencing technology and a growing awareness of the virome. Several in vitro and in vivo studies indicate that ISVs are capable of modulating pathogenic arboviruses. In addition, there is growing evidence that both vertical and horizonal transmission strategies maintain ISVs in vector populations. As such there is potential to exploit ISVs for stand-alone vector control strategie...
Abstract: Jumping spiders (Salticidae) typically prey on a variety of arthropods of similar size to themselves, but rarely on ants. Using 28 salticid species from East Africa, we frst investigated vision-based aversion to ants by recording latency to enter a transparent sealed chamber fanked by chambers containing living army ants (Dorylus sp.) or tsetse fies (Glossina pallidipes) of comparable size. For all species, entry latency was signifcantly longer when the stimuli were ants. In anothe...
Abstract: Field observations have demonstrated that gravid Anopheles gambiae Giles s.s. (Diptera: Culicidae) are selective in their choice of oviposition sites. For example, immature stages of An. gambiae s.s. are rarely found in water that contains Culex quinquefasciatus Say immatures. The possibility that this may, in part at least,reflect a response by ovipositing An. gambiae s.s. females to volatile signals associated with Culex juveniles was evaluated by testing the response of An. gamb...
Abstract: At six sites in western Kenya, we explored the presence of Anopheles immature stages in treeholes. An. gambiae larvae were found in 19 species, 13 of which are exotic. The most common exotic species were Delonix regia, Jacaranda mimosipholia, and Eucalyptus citrodora. In Kisumu city, longitudinal assessments of 10 flamboyant trees showed repeated presence of An. gambiae s.s. in treeholes with water. Production of Anopheles larvae did not correlate with habitat volume but with habit...