Introduction Definitions of Some Terms Basic Principles of Experimental Design Objectives of an Experiment Analysis Of Variance (ANOVA) Completely Randomized Design Layout of Responses from CRD Model for CRD ANOVA TABLE Statement of Hypotheses Decision Rule UNEQUAL REPLICATIONS
Introduction Correlation Analysis Scatter Plot Correlation Coefficient Properties Pearson’s Product Moment Correlation (PPMC) Coefficient Spearman’s Rank Correlation (SRC) Coefficient Test of Significance of the Correlation Coefficient Procedure Regression Analysis
Determination of Sample Size Confidence Intervals for Small Sample Size with Unknown Population Variance Confidence Intervals for a Population Proportion Determination of Sample Size for Estimating a Proportion
Characteristcs of Viruses Classification of Viruses Stages in Viral Multiplication Methods of Studying Viruses Cultivation of Bacteriophage Cultivation of Animal Viruses Tissue/Cell Culture
STRUCTURE OF PROKARYOTIC CELL Cytoplasmic Membrane and Cytoplasm Nucleoid/Genetic material Ribosomes Mesosomes Plasmid Glycocalyx(Capsule and Slime Layer) Flagella Fimbriae and Pili Cell wall
Cytoplasmic Membrane Cytoplasm Cell Wall Cytoskeleton Nucleus Ribosome Endoplasmic Reticulum Golgi Apparatus Mitochondria Lysosomes Peroxisomes Chloroplasts Vacuoles
BACTERIAL CLASSIFICATION CLASSIFICATION BASED ON SHAPE CLASSIFICATION BASED ON GRAM REACTION CLASSIFICATION BASED ON GROWTH REQUIREMENT OXYGEN REQUIREMENT TEMPERATURE CARBON AND ENERGY SOURCE BACTERIAL STRUCTURE AND FUNCTION EXTERNAL CELL STRUCTURES CELL WALL Functions of the Cell Wall GLYCOCALYX Functions of the Glycocalyx FLAGELLA Functions of the Flagella FIMBRIAE AND PILI Functions AXIAL FILAMENTS INTERNAL STRUCTURES PLASMA MEMBRANE Functions of the Plasma Membrane CYTOPLASM NUCLEOID RIBO...
Microbiology Structure & Organisation of Prokaryotic & Eukaryotic Cell What is Cell Theory of Cell Fungi Characteristics of Fungi Nomenclature & Classification of Fungi Morphology of Fungi Types of Septum in a Septate Hyphal Modification of Mycelium Nutrition in Fungi Reproduction in Fungi Types of Reproduction Types of Asexual Spores Sex Organelles of Fungi Types of Sexual Spores Fruiting Bodies Comparative Physiology of Fungi & Bacteria General Methods of Studying Fungi Algae & Lichen Liche...
Introduction Purpose Materials required Experiment 2: Blood Agar Preparation Experiment 3: Preparation of Potato Dextrose Agar(PDA)
Isolation of Microorganisms Methods of Isolation Cultivation of Microorganisms
Major Tools for Identification of Micro-organisms 1. Microscope. 2. What is a bacterium? 3. Identification of Fungi Molecular method of identification of microorganisms -Isolation of DNA -polymerase chain reaction -purification of PCR product -Nucleotide sequencing -Blasting of the analysis extraction
PROCEDURES: Preparation of Solid Media PREPARATION OF CULTURES
1. What is pasteurization? Pasteurization is the partial sterilization of a product, to make it safe for consumption and improve it's sustainability e.g Milk or wine. b. What is tyndallization? Tyndallization is the process by which food or beverage is heated in order to kill heat resistant endospores.
Isolation of microorganisms Methods of isolation of bacteria Method of inoculating the solid culture media
Introduction Kinetics of Microbial Death Term in Quantifying Sterilization Process Sterilization with Heat Sterilization with Chemical Sterilization with Radiation Sterilization with Filtration