Natural & Applied Sciences

Natural & Applied Sciences Research Papers/Topics

Beyond sperm and male accessory gland proteins: Exploring insect reproductive metabolomes

Abstract: Insect seminal fluid, the non-sperm component of the ejaculate, comprises a variegated set of molecules, including, but not limited to, lipids, proteins, carbohydrates, salts,hormones, nucleic acids, and vitamins. The identity and functional role of seminal fluid proteins (SFPs) have been widely investigated, in multiple species. However, most of the other small molecules in insect ejaculates remain uncharacterized. Metabolomics is currently adopted to deepen our understanding of c...

Exposure to Salinity and Light Spectra Regulates Glucosinolates, Phenolics, and Antioxidant Capacity of Brassica carinata L. Microgreens

Abstract: The effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate ...

Immunotoxic effects of force-fed ethephon on model organism Galleria mellonella (Lepidoptera: Pyralidae)

Abstract: Cytogenotoxic effects of a commercial formulation of ethephon (ETF) and a commercially formulated mixture of ethephon+cyclanilide (ETF+CYC), which are used as plant growth regulators (PGRs) in modern agriculture, were investigated by using the anaphase-telophase chromosome aberration (CA) assay in root meristem cells of Allium cepa L. A. cepa roots were treated with 25 (EC50/2), 50 (EC50), and 100 ppm (EC50×2) of ETF, and with the same concentrations of ETF+CYC, for 24, 48, and 72...

Chemical Cues From Honeydew and Cuticular Extracts of Trialeurodes Vaporariorum Serve as Kairomones for The Parasitoid Encarsia Formosa

Abstract: Kairomones are semiochemicals that are emitted by an organism and which mediate interspecific interaction that is of benefit to an organism of another species that receives these chemical substances. Parasitoids find and recognize their hosts through eavesdropping on the kairomones emitted from the by-products or the body of the host. Hemipteran insect pests feed on plant sap and excrete the digested plant materials as honeydew. Honeydew serves as a nutritional food source for para...

Comparative microbiome analysis of Diaphorina citri and its associated parasitoids Tamarixia radiata and Diaphorencyrtus aligarhensis reveals Wolbachia as a dominant endosymbiont

Abstract: Microbiome analysis in a host–parasitoid interaction network was conducted to compare the taxonomic composition of bacterial communities of Diaphornia citri, Tamarixia radiata, and Diaphorencyrtus aligarhensis. The comparative analysis revealed differences in the composition and diversity of the symbiont populations across the host and its associated parasitoids. Proteobacteria was the most dominant phylum, representing 67.80% of the total bacterial community, while Candidatus Pr...

Disentangling thermal effects using life cycle simulation modelling on the biology and demographic parameters of Dolichogenidea gelechiidivoris, a parasitoid of Tuta absoluta

Abstract: Dolichogenidea gelechiidivoris (Marsh) (Syn. Apanteles gelechiidivoris) (Hymenoptera: Braconidae), a konoibiont larval endoparasitoid of the South American tomato pinworm Tuta absoluta (Meyrick), was imported into Kenya in 2017 for the first classical biological control of T. absoluta in Africa. We determined the thermal thresholds of D. gelechiidivoris, with T. absoluta as host, using life cycle simulation modelling. Life-table data of D. gelechiidivoris were generated at six cons...

The parasitoid Dolichogenidea gelechiidivoris eavesdrops on semiochemicals from its host Tuta absoluta and tomato

Abstract: Semiochemicals such as herbivore-induced plant volatiles (HIPVs) and host chemicals serve as communication signals for parasitoids searching for oviposition sites. The braconid koinobiont endoparasitoid Dolichogenidea gelechiidivoris (Hymenoptera: Braconidae) efficiently parasitises larvae of Tuta absoluta (Lepidoptera: Gelechiidae), a major pest of tomato (Solanum lycopersicum). However, the attractive compounds used by the parasitoid to locate T. absoluta on host plants are not k...

Amplicon-based assessment of bacterial diversity and community structure in three tropical forest soils in Kenya

Abstract: Forest soils provide a multitude of habitats for diverse communities of bacteria. In this study, we selected three tropical forests in Kenya to determine the diversity and community structure of soil bacteria inhabiting these regions. Kakamega and Irangi are rainforests, whereas Gazi Bay harbors mangrove forests. The three natural forests occupy different altitudinal zones and differ in their environmental characteristics. Soil samples were collected from a total of 12 sites and so...

Mosquito Larval Habitats in Semiarid Ecosystem in Eritrea: Impact of Larval Habitat Management on Anopheles arabiensis Population.

Abstract: This study investigated the impact of larval management and the temporal variation in larval productivity in Eritrea, a semiarid ecosystem. Results of this study show that mosquito breeding persists throughout the year mainly in stream bed pools. Anopheles arabiensis production in the ephemeral natural aquatic habitats such the streambed pools was high throughout the year and negatively associated with rainfall (r −0.288, P 0.047). High densities of An. arabiensis larvae were als...

Convergent Evolution of eye Ultrastructure and Divergent Evolution of Vision-mediated Predatory Behaviour in Jumping Spiders

Abstract: All jumping spiders have unique, complex eyes with exceptional spatial acuity and some of the most elaborate vision-guided predatory strategies ever documented for any animal of their size. However, it is only recently that phylogenetic techniques have been used to reconstruct the relationships and key evolutionary events within the Salticidae. Here, we used data for 35 species and six genes (4.8 kb) for reconstructing the phylogenetic relationships between Spartaeinae, Lyssomanina...

Assessment of Resistance Mechanisms to Fall Armyworm, Spodoptera frugiperda in Tropical Maize Inbred Lines

Abstract: The fall armyworm (FAW), Spodoptera frugiperda, a pest of maize native to the Americas first reported in West and Central Africa in 2016, severely threatens maize production and food security in Sub-Saharan Africa. Native genetic resistance is one of the best methods of control of insect pests as it is contained in the seed making it more amenable for use by farmers compared to other interventions and it is also compatible with other integrated pest management (IPM) options. An int...

Decision support system for fitting and mapping nonlinear functions with application to insect pest management in the biological control context.

Abstract: The process of moving from experimental data to modeling and characterizing the dynamics and interactions in natural processes is a challenging task. This paper proposes an interactive platform for fitting data derived from experiments to mathematical expressions and carrying out spatial visualization. The platform is designed using a component-based software architectural approach, implemented in R and the Java programming languages. It uses experimental data as input for model fi...

Risk assessment of urban yellow fever virus transmission in Kenya: is Aedes aegypti an efficient vector?

Abstract: The absence of urban yellow fever epidemics in East Africa remains a mystery amidst the proliferation of Aedes aegypti in this region. To understand the transmission dynamics of the disease, we tested urban (Mombasa, Kisumu, and Nairobi) Aedes mosquito populations in Kenya for their susceptibility to an East African yellow fever virus (YFV) genotype. Overall, 22% (n = 805) of the Ae. aegypti that were orally challenged with an infectious dose of YFV had a midgut infection, with...

Multitrophic Interaction Facilitates Parasite–host Relationship Between an Invasive Beetle and the Honey Bee.

Abstract: Colony defense by honey bees, Apis mellifera, is associated with stinging and mass attack, fueled by the release of alarm pheromones. Thus, alarm pheromones are critically important to survival of honey bee colonies. Here we report that in the parasitic relationship between the European honey bee and the small hive beetle, Aethina tumida, the honey bee’s alarm pheromones serve a negative function because they are potent attractants for the beetle. Furthermore, we discovered that ...

Disconcerting trends in populations of the endangered Sokoke Scops Owl Otus ireneae in the Arabuko-Sokoke Forest, Kenya

Abstract: The Sokoke Scops Owl Otus ireneae is an East African endemic that occurs mainly in the Cynometra woodland of the Arabuko-Sokoke Forest (ASF) in coastal Kenya. Since its discovery in 1965 (Ripley 1966), two additional subpopulations were discovered; in 1992 a small population was discovered in the foothill forests of the east Usambara mountains in Tanzania (Evans et al. 1994),and in 2002 another small population was discovered in the Dakatcha woodlands, 30 km north of ASF (C Jackson...


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