Natural & Applied Sciences

Natural & Applied Sciences Research Papers/Topics

MICROPROPAGATION OF ENDOD (Phytolacca dodecandra) L ′Herit THROUGH LEAF CULTURE

Abstract: Endod (Phytolacca dodecandra L’Heit var. E-44 and E-17) is medicinal plant of high saponin content used as molluscicide to control Schistosomiasis. The aim of, this study was to develop a micropropagation protocol for P.dodecandra. The E17 and E44 variety leaf explant was sterilized and cultured on MS media supplemented with different types of concentration and combination of cytokinins and auxins for shoot initiation, multiplication and root initiation. Local bleach (Berekina) 3...

PHYTOCHEMICAL SCREENING FROM LEAVES OF DATURA STRAMONIUM L. AND JUSTICIA SCHIMPERIANA AND EVALUATION OF THEIR ANTIMICROBIAL PROPERTIES AGAINST SELECTED HUMAN ENTERIC BACTERIA

Abstract: The growing phenomenon of antibiotic resistance, particularly to pathogenic microorganisms, in current medicine, has directed the concern of scientists for finding novel antimicrobial agents from plant origin with negligible side effect. The aim of this study was to screen the major secondary compounds of leaves of D. stramoinum and J. schimperiana and to evaluate their antimicrobial properties against (S. aureus, S. typhi and S. boydii). Extraction was done by maceration of leaf p...

FREQUENCIES OF THE ABO BLOOD GROUP SYSTEM AND ITS RELATIONSHIP WITH Helicobacter pylori INFECTION AMONG PATIENTS WITH GASTROINTESTINAL COMPLAINTS IN ASELLA TEACHING AND REFERRAL HOSPITAL, ARS

Abstract: Helicobacter pylori are a small, spiral-shaped bacterium that lives on the surface of the stomach and duodenum. H. pylorus is closely associated with onset of diseases such as gastric ulcers, duodenal ulcers, gastritis and gastric cancers. The aim of this study was to determine the frequencies of ABO blood group system and its relationship with H. pylori infection among patients with gastrointestinal complaints at the Asella Teaching and Referral Hospital. A total of 422 patients o...

PHYTOTOXICITY OF Lantana camara L. EXTRACTS AND SOIL FROM BENEATH ITS CANOPY ON CABBAGE (Brassica carinata A.)

Abstract: Lantana camara is an invasive weed species that has allelopathic effects on crops and other indigenous associated plants. Allelochemicals from such plants may also have positive or negative impact on soils. Therefore, the research was carried out to investigate the allelopathic effect of leaf extracts of Lantana camara and soils invaded by it on Brassica carinata. Leaf extracts of Lantana camara were prepared with water, hexane and methanol, while distilled water alone was used as ...

ABO BLOOD GROUP DISTRIBUTION AND ITS RELATIONSHIP WITH SOME INTESTINAL PROTOZOAN PARASIC INFECTIONS IN WARA JARSO DISTRICT, NORTH SHEWA ZONE, OROMIA REGIONAL STATE,ETHIOPA

Abstract: The Frequencies of ABO blood groups system phenotypes,alleles, and genotypes vary worldwide and are not found in equal frequencies even among ethnic groups.Part of the immune response is the production of globulins called antibodies to combat the invader.ABO blood group system has multiple associations with different infectious diseases including intestinal protozoan parasitic infections (IPPIs). Therefore, the aim of this study was to assess the distribution of ABO blood group sys...

ETHANOL PRODUCTION FROM SORGHUM STRAW WITH ACID PRETREATMENT USING Saccharomyces cerevisiae

Abstract: Fermentation is a metabolic process of microorganisms to obtain energy by breaking down organic compound. Sorghum straw was fermented with different concentration substrate (15gm, 30gm , 45g and 60gm) with 0.5% ,1% yeast in different fermentation period (4th day, 8th day, 12th and 16th days) with the aim of ethanol production from Sorghum straw. Among the different substrate concentration 15 gram of substrate mixed with 1% yeast produced high quantity of ethanol on 4th day of ferme...

ISOLATION AND CHARACTERIZATION OF POTENTIAL PROBIOTIC LACTIC ACID BACTERIA FROM THE GASTROINTESTINAL TRACT (GIT) OF POULTRY

Abstract: Lactic acid bacteria are one of the most important groups of microorganisms useful in animal and human health as probiotics. Probiotics are the health promoting living microorganisms and produces antimicrobial compounds such as organic acids, H2O2 and bacteriocins. Now a day, the use of antibiotics as growth promoters is questionable. In this case, probiotics have a potential to solve this problem as one of the alternatives to antibiotic growth promoters. The aim of this study was ...

PREVALENCE AND INTENSITY OF INTESTINAL PARASITE INFECTIONS AMONG CHILDERN AT MUKETURI PRIMARY SCHOOL, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: Intestinal parasitic infections (IPI) are among the major health problems in many countries including Ethiopia. The objective of the present study was to determine the prevalence and intensity of intestinal parasitic infections and to identify the major protozoan and helminthes parasitic species among primary school children in the study area .The design of the study was a ccross-sectional school based parasitological survey involving 400 sample student populations drawn from Muket...

CURRENT PREVALENCE AND TRENDS OF MYCOBACTERIUM TUBERCULOSIS AND HIV INFECTIONS AND CO-INFECTIONS AMONG PATIENTS VISITING KUYU HOSPITAL, GARBA GURACHA TOWN, NORTH SHOA, OROMIA, ETHIOPIA

Abstract: HIV greatly increase the number of TB patients, which in turn increases TB transmission from family member and community members. The study was conducted in Kuyu Hospital from February-May 2017 with the objectives of determining the prevalence and trends of HIV, TB and their co-infections and associated risk factor among patients visiting Kuyu Hospital. A total of 384 patients of all age were enrolled in the study and out of this, the number of males and females were 196 and 188, r...

EVALUATION OF CELLULASE PRODUCTION BY RUMEN BACTERIA USING LIGNOCELLULOSIC SUBSTRATES AS CARBON SOURCE

Abstract: The rumen is a special digestive vessel of ruminants, where digestion of cellulose and other plant polysaccharides occurs through microbial activities. On average, cellulose accounts for 50% of the dry weight of plant biomass. Cellulose and agricultural wastes were used as sole carbon sources for the production of cellulase by bacteria. This study focused on isolating and screening cellulase-producing rumen bacteria. The isolated rumen bacteria were identified using morphological a...

BUTTER PRESERVATIVE POTENTIALS OF SELECTED SPICES AND HERBS FROM OROMIA REGIONAL STATE, WEST ARSI ZONE, SHASHEMANIE WOREDA, ETHIOPIA

Abstract: In Ethiopia, it has been reported that there are no official quality control standards and cooling and other preservative facilities for most dairy products. Under such circumstances, fresh raw butter which has pleasant taste and odor will lose its quality because of microbial contamination. Hence, this study was conducted with the objective of investigating the butter preservation potential of selected spices/herbs commonly used in traditionally processed butter around Shashemenie...

THE EFFECT OF Aloe vera Mill. GEL COATING ON POSTHARVEST QUALITY OF AVOCADO (Persea americana MILL.) FRUITS

Abstract: Aloe vera gel has been proven to be one of the best edible and biologically safe preservative coatings for different types of foods because of its film-forming properties, antimicrobial actions, biodegradability and biochemical properties. This study was planned to investigate the role of aloe gel coating in improving post harvest quality, shelf life, sugar and antioxidant (carotenoid) content of avocado fruit. The experiment was laid in completely randomized design (CRD) in two re...

PREVALENCE OF INTESTINAL PARASITIC INFECTIONS AND THEIR ASSOCIATIONS WITH RISK FACTORS AND ANTHOROPOMETRIC MEASUREMENTS OF SCHOOLCHILDREN IN ABOKER MUTI PIMARY SCHOOL, HARARI REGIONAL STATE,

Abstract: Intestinal parasitic infections are the major public health problems in many developing countries including Ethiopia. The objective of the study was to determine the prevalence of intestinal parasitic infection among Aboker Muti Primary SchoolChildren. A descriptive cross-sectional survey was carried out involving 384 schoolchildren who were selected using stratified random sampling technique during November 2018 to January 2019. A structured and pre-tested questionnaire were admin...

ISOLATION OF Aspergillus oryzae AND SINGLE CELL PROTEIN PRODUCTION BY GROWN ON COFFEE CHERRY HUSK AND SAWDUST

Abstract: The rising global population pressure generates challenges to fulfill food requirements for the coming generations in adequate amounts. This suggests that the human population cannot continue to be entirely dependent on crops, animal husbandry and fisheries for food. Instead, efforts should be made to enhance the production of other potential alternative sources of food. To this end the current study has been undertaken to evaluate the production of single cell protein (SCP) from A...

Production and Characterization of Pectinase from Pectinolytic Fungi Cultivated on Mango peels and Pectin Subjected to Submerged Fermentation

Abstract: Pectinases are the group of enzymes that degrade pectin. This study was conducted with the aim of isolation of efficient pectinase producing pectinoloytic fungi from the decaying vegetable and fruit soil containing decomposing mango peels using extracted mango peels pectin as a growth substrate under submerged fermentation, determining optimum pectinase production conditions and characterizing of ammonium sulphate precipitated pectinase activity with regards to some physicochemical...


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