INTRODUCTION There is no Health without “MENTAL HEALTH” but many people seldom talk and some only pay little attention to doing things to promote their mental health. Many people who have mental health challenges may not even know that they have such not to talk of seeking help! Hello, welcome to this course. We are happy to have you doing NSC 314 – Mental Health and Psychiatric Nursing I. You would have done some lectures in Mental Health and Psychiatric Nursing when you were in the ba...
This paper looks at a movement-based assay to analyze adaptability in Caenorhabditis elegans locomotion behavior. A series of sinusoidal microchannels of fixed wavelength and varying amplitude, with the width being similar to the body diameter of the organism, are used for this purpose. The worms are allowed to enter the channel from the input and transition towards the output port. In sections that are similar to the worms' undulations, the movement is fast and steady. However, as the channe...
This paper looks at a movement-based assay to analyze adaptability in Caenorhabditis elegans locomotion behavior. A series of sinusoidal microchannels of fixed wavelength and varying amplitude, with the width being similar to the body diameter of the organism, are used for this purpose. The worms are allowed to enter the channel from the input and transition towards the output port. In sections that are similar to the worms' undulations, the movement is fast and steady. However, as the channe...
This study uses the model organism, C. elegans, to investigate its sensitivity and response to static magnetic fields. Wild-type C. elegans are put into microfluidic channels and exposed to permanent magnets for five cycles of thirty-second time intervals at field strengths ranging from 5 milli Tesla to 120 milli Tesla. Recorded and analyzed with custom software, the results of the worm's movement - the average velocity, turning and curling percentage - were compared to control experiments...
To evaluate the level of infestation of the soybean cyst nematode (SCN), Heterodera glycines, in the field, egg population densities are determined from soil samples. Sucrose centrifugation is a common technique for separating debris from the extracted SCN eggs. We have developed a procedure, however, that employs OptiPrep as a density gradient medium, with improved extraction and recovery of the eggs compared to the sucrose centrifugation technique. Also, we have built computerized methods t...
The transmembrane proteins known as ion channels play a role in controlling and preserving the ionic concentrations across the cell membrane. Modeling the flux of ions in and out of these channels on an atomic level is essential for understanding several neurological diseases and related pharmaceutical discoveries. Recent experimental research has provided information on the channel's physical structure which can be used to create realistic ion transport models. Different trajectories exist f...
The transmembrane proteins known as ion channels play a role in controlling and preserving the ionic concentrations across the cell membrane. Modeling the flux of ions in and out of these channels on an atomic level is essential for understanding several neurological diseases and related pharmaceutical discoveries. Recent experimental research has provided information on the channel's physical structure which can be used to create realistic ion transport models. Different trajectories exist f...
Melanoma is one of the deadliest types of skin cancer and can be difficult to treat when it's advanced. To reduce mortality rates, early detection is key. In order to do this, computer-aided systems have been developed to help dermatologists diagnose the condition. To make it more accessible to the public, researchers are working on creating portable, at-home diagnostic systems. An Android-based smartphone application utilizing image capture, preprocessing, and segmentation was developed to e...
By utilizing a low-cost engineering tool, we have created a microfluidic platform to study bacteria at the single cell level, allowing us to unlock insights into microbial physiology and genetics that would otherwise not be possible. The platform is composed of 3D devices made of adhesive tapes, an agarose membrane as the resting substrate, a temperature-controlled environmental chamber, and an autofocusing module. With this technology, we have been able to observe Escherichia coli morphologi...
In this paper, we explore the use of microfluidic paper-based analytical devices (lPADs) to study the behavior of Caenorhabditis elegans. We show how these devices can be fabricated on paper and plastic substrates, as well as how to load, visualize, and transfer single and multiple nematodes. We also demonstrate the use of anthelmintic drug, levamisole, to perform chemical testing on C. elegans. Furthermore, we provide a custom program that is able to recognize individual worms on the lPADs i...
To evaluate the level of infestation of the soybean cyst nematode (SCN), Heterodera glycines, in the field, egg population densities are determined from soil samples. Sucrose centrifugation is a common technique for separating debris from the extracted SCN eggs. We have developed a procedure, however, that employs OptiPrep as a density gradient medium, with improved extraction and recovery of the eggs compared to the sucrose centrifugation technique. Also, we have built computerized methods t...
To evaluate the level of infestation of the soybean cyst nematode (SCN), Heterodera glycines, in the field, egg population densities are determined from soil samples. Sucrose centrifugation is a common technique for separating debris from the extracted SCN eggs. We have developed a procedure, however, that employs OptiPrep as a density gradient medium, with improved extraction and recovery of the eggs compared to the sucrose centrifugation technique. Also, we have built computerized methods t...
We address the current situation of population-wide and speedy testing for SARS-CoV-2 and review different technologies available, such as molecular and rapid antigen tests, serology and antibody tests, and tests for managing COVID-19 patients. We consider products authorized by the FDA to diagnose and detect COVID-19 infections. This review covers pooled testing, screening programs, and surveillance testing to enable quicker screening. We also discuss the potential challenges in terms of det...
We address the current situation of population-wide and speedy testing for SARS-CoV-2 and review different technologies available, such as molecular and rapid antigen tests, serology and antibody tests, and tests for managing COVID-19 patients. We consider products authorized by the FDA to diagnose and detect COVID-19 infections. This review covers pooled testing, screening programs, and surveillance testing to enable quicker screening. We also discuss the potential challenges in terms of det...
Bacterial populations grown in a lab environment are typically seen as homogeneous in terms of growth rate, size, and content. However, recent findings have shown that even during exponential growth phase, these cells can be heterogeneous. This has important consequences for how bacteria respond to stressors such as antibiotics. For example, the concept of antimicrobial persistence is linked to a small subpopulation of cells that enter a dormant state where antibiotics are no longer effective...