Natural & Applied Sciences

Natural & Applied Sciences Research Papers/Topics

ISOLATION AND IDENTIFICATION OF BACTERIA FROM FOOD VENDORS AND SOME VEGETABLE AVAILABLE AT OGBETE MARKET ENUGU

ABSTRACTSafety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green), were randomly purchased from five different vendors in Ogbete main market Enugu. The samples were transported in ice to the laboratory. The samples were bacteriologically analyzed using pour plate technique and sub-culture. Pour plate techniques was done by carrying out serial dilution of the sample after which the first tube and the last tube...

HEPATITIS C VIRUS AMONG PREGNANT WOMEN PEOPLE LIVING WITH HIV AIDS ATTENDING CLINIC AT UNTH ITUKUOZALLA

ABSTRACTA total of 50 blood (30 pregnant and 20 HIV) samples were obtained from pregnant women and PLWHA attending clinic at UNTH Ituku-ozalla. Samples were screened for hepatitis c viral infection using the rapid one step hepatitis C virus test strip. Two (6.66%) pregnant women were positive and four (20%) HIV patients were positive for Hepatitis C, giving an overall prevalence rate of (26.7%). The infection was the same in male (3) and in female (3). Those aged 23-34 years recorded a higher...

EFFECT OF DIFFERENT CARBON SOURCES ON THE GROWTH OF ANTIMICROBIAL PRODUCING Bacillus Species IN Citrillus vugaris.

ABSTRACTEffect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. ...

COMPARATIVE ANALYSIS OF PIPEBORNE WATER AND OTHER SOURCES OF WATER WITHIN ENUGU METROPOLIS FOR HUMAN CONSUMPTION.

ABSTRACTComparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determine their levels of contamination of bacteria. The sources of water examined are tap water, well water and stream water. The pour plate method was used to examine the water samples. Bacteria isolated from the water samples include coliforms especially Escherichia coli. Stream water and well water were found to contain bacterial isolates unlike the tap water t...

BIOCONTROL POTENTIAL OF BACILLUS THURINGIENSIS ISOLATED FROM SOIL SAMPLES AGAINST LARVA OF MOSQUITO

ABSTRACTA major challenge for achieving successful mosquito control is overcoming insecticide resistance. Bacillus thuringiensis which is one of the most effective biolarvacide for control of species of mosquitoes and monitoring of larval susceptibility is essential to avoid resistance development. Mosquito larvacidal activity of Bacillus thuringiensis was assessed by isolating them from ecologically different soil habitats in and around Enugu metropolis. The isolate organisms were confirmed ...

ANTIBACTERIAL ACTIVITY OF HONEY ON Staphylococcus aureus Escherichia coli and Streptococcus pyogen ISOLATED FROM WOUND

ABSTRACTAntibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing hi...

BACTERIOLOGICAL ASSESSMENT OF DRINKING WATER IN ANKPA LOCAL GOVERNMENT, KOGI STATE

AbstractWater is colourless, tasteless, and odorless substance that is essential to all form of life. (The Anglo-Saxon and low German Word, Water (1993).According shittu etal, (2008) water is vital to our existence in life and its importance in our daily life makes it imperative that through microbiological and physiochemical examination be conducted on water.Water is essential to the continues existence of all living organism but it is increasely been threatened as human population grows and...

PROXIMATE AND MINERAL COMPOSITION OF THEOBROMA CACAO BEAN AND SHELL

ABSTRACTThe demand for cocoa beans is growing immensely in the world today due to the increasing consumption of cocoa butter, syrups, pastes and all kinds of chocolates, resulting in the rising of price of beans, which in term encouraging the farmers to increasing their productivity. The cocoa beans used in this project work were obtained from IPELE Farm Owo, in Ondo State, Nigeria. The cocoa beans were sundried after fermentation for 5days and dishelmed. The bean and shell were separately mi...

THE ANTI-INFLAMMATORY ACTIVITY OF Crateva adansonii DICHLOROMETHANE FRACTION

ABSTRACTCrateva adansonii is a medicinal herb commonly used in parts of Africa because of the side effects of Non-Steroidal Anti-inflammatory Drugs (NSAID), like heart diseases and kidney failure. Inflammation is a major public heart issue in the world but treatment is becoming complex because of the side effects of anti-inflammatory pharmaceutical drugs. Hence the need for alternative drug is highly required. This research work investigated the anti-inflammatory activity of dichloromethane f...

THE EFFECT OF N-HEXANE EXTRACT OF KOLA NITIDA BARK ON LIVER FUNCTION TEST OF ALBINO WISTAR RATS FED WITH HIGH FAT FROM COW‘S BRAIN.

ABSTRACTKola bark extract have been widely used in tradition medicine for thousand of year, it improves liver functions and provides protection against high fat fed metabolic rats. Present investigations were carried out on the hepatoprotective role of Kola bark extract meal treatment to high fat fed wistar rat. Healthy adult male wistar rats were divided into four groups Group I: rats were fed a standard Laboratory diet (groups match) (20g/rat/day), Group II: rats were fed a high-fat diet al...

PRELIMINARY INVESTIGATION ON EFFECTS OF BURANTASHI EXTRACT ON LIVER ENZYMES OF AIBINO MALE AND FEMALE WHISTAR RATS.

ABSTRACTThis work was carried out to investigate the effects of Burantashi extract on liver enzymes of albino male and female whistar rats. Burantashi is a popular seasoning agent to barbecued meat (suya) in Nigeria,mostly found in the northern part of the Nigeria. Liver Enzymes are those enzymes that plays important role in the liver both in function and regulation. Erectile dysfunction (ED) is defined as the consistent or recurrent inability of a man to attain or maintain penile erection, s...

THE EFFECT OF ETHANOL EXTRACT OF DESMODIUM VELUTINUM STEM ON SOME MARKER EMZYME IN LIVER OF ALBINO WISTAR RATS.

AbstractPresent investigations were carried out on the effect of ethanol extract of Desmodium velutinum stem on some liver enzymes of albino wistar rats fed with high fat from cow‟s brain. Twelve healthy albino wistar rats were divided into four groups. Group I rats were fed with balanced diet (normal feed, that is grower‟s mash) and tap water. Group II rats were fed with fat diet (cow‟s brain) (3.6g/day) alone. Group III rats received combined mixture of 70% fat diet (cow‟s brain) wi...

PRELIMINARY INVESTIGATION ON EFFECTS OF BURANTASHI EXTRACT ON LIPOPROTEINS OF ALBINO MALE AND FEMALE WHISTAR RATS.

ABSTRACTThis work was carried out to investigate the effects of Burrantashi extracts on the lipoproteins Burantashi is a popular seasoning agent to barbecued meat (Suya) in Nigeria. Found in the northern parts of the country. Lipoproteins are the principal steroid or fat that is synthesized in the liver or intestines of animals. Erectile dysfunction (ED) is defined as the consistent or recurrent inability of a man to attain or maintain penile erection, sufficient for sexual activity (2nd inte...

THE EFFECT OF PTEROCARPUS MILDBREADII SEED ON PLASMA HDL CHOLESTEROL OF ALBINO RAT.

ABSRACTThis work was carried out to investigate the effects of pterocarpus mildbreadii seed on the HDL- level of albino rats.High density lipoprotein(HDL) is a fraction of cholesterol,which helps to transport cholesterol from peripheral of cell to the liver.High density lipoprotein is carried out to investigate cardio vascular diseases.Fresh oha seed (pterocarpus mildbreadii seed) was collected from Amokwe in udi local government Area Enugu state. They were dried at room temperature for about...

PHYTOCHEMICAL AND ANTI-INFLAMMATORY PROPERTIES OF METHANOL EXTRACT OF CRATEVA ADANSONII STEM BARK.

ABSTRACTThis research investigated the phytochemical and anti-inflammatory properties of methanol extract of Crateva adansonii stem bark. Although several edible and non-edible plants parts are used in inflammatory treatment, many record has been found of the use of Crateva adansonii stem bark. For this research, fresh stem bark of Crateva adansonii were collected from Asata village in Enugu State. The cuttings were authenticated at the Bioresource development centre. They were then dried at ...


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