Natural & Applied Sciences

Research Papers/Topics Natural & Applied Sciences

ALLELIC AND GENOTYPIC FREQUENCIES OF ABO AND Rh BLOOD GROUP AMONG STUDENTS ATTENDING TULU CHUKALA SECONDARY SCHOOL, ADULALA TOWN, EAST SHOA ZONE, OROMIA REGIONAL STATE, ETHIOPI

Abstract: The four blood types are known as A, B, AB, and O. Blood type A contains A antigen on the surface of red blood cells and B antibody on blood plasma. Type B blood contains the reverse combination. Serum of blood type AB contains neither antibody, but red cells in this type of blood contain both A and B substances. In type O blood, neither substance is present on the red cells, but the individual is capable of forming antibodies directed against red cells containing substance A nor B...

MICROBIAL CONTAMINATIONS OF MEAT AND ASSOCIATED RISK FACTORS IN BEKOJI TOWN, ARSI ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: The consumption of raw meat has associated with cultural practices and widespread raw beef consumption habit that can be a potential source for food borne illnesses in Ethiopia. Therefore, the present study was intended to assess microbiological contamination and associated risk factors in Bekoji town. A cross sectional study was employed whereby a simple random sampling of Butcher shops in Bekoji Town. The cattle (muscle, liver, and intestine) meat samples were collected aseptical...

EFFECTS OF NEEM LEAF EXTRACT DIPPING AND BEESWAX COATING ON SHELF LIFE AND QUALITY OF TOMATO (Lycopersicon esculentum Mill.) FRUITS

Abstract: Tomato fruit is highly perishable due to its climacteric ripening nature. The high postharvest loss of tomato in Ethiopia is also because of poor harvesting techniques, lack of packaging, inappropriate transportation, storage and absence of cooling facilities. Experiment was carried out to investigate the efficacy of beeswax (BW) emulsion and neem leaf extract (NLE) on elongation of shelf life and maintenance of quality of harvested tomato fruits. Freshly harvested mature green tom...

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC FUNGI FROM FENNEL (Foeniculum vulgare Mill.) LEAF AND SEED

Abstract: Antioxidant and antimicrobial properties are the biological activity of natural compoundsisolated from medicinal plants that are rich source of novel drugs. Endophytic fungi are a potential source of novel bioactive compounds. The present study investigates antimicrobial and antioxidant properties of endophytic fungi from fennel (Insilali) seeds and leaves. The antioxidant activity was conducted based on determination of phenolic content, 1, 1-diphenyl-2-picrylhydrazyl and hydrogen...

THE EFFECT OF SALT STRESS ON GROWTH PERFORMANCE AND FRUIT YIELD OF TOMATO (LYCOPERSICON ESCULENTUM MILL.) VARIETIES

Tomato leaf, shoot height and stem diameter reduced under salinity stress caused by photosynthesis reduction, tissues expansion reduction and cell divided inhibition. Salinity also reduces leaf chlorophyll content, stomatal resistance and photosynthetic activities

ETHNOBOTANICAL STUDY OF TRADITIONALMEDICINALPLANTS IN LUME DISTRICT EASTERN SHOA ZONE OROMIA REGIONAL STATE ETHIOPIA

Abstract: Ethnomedicinal research plays an important role for conservation and sustainable utilization of medicinal plants and associated knowledge. The objective of this study was to document medicinal plants and associated indigenous knowledge in Lume District. Ethnobotanical data was collected from February 2022 to September 2023 using semi-structured interviews, focus group discussion and direct field observation with 76 randomly selected non-key informants and 10 key informants. The col...

PREVALENCE AND INTENSITY OF HUMAN INTESTINAL PARASITES IN FECTIONS AND ASSOCIATED RISK FACTORS AMONG CHILDREN IN NICOLAS BAM PRIMARY SCHOOL, HARARI REGION EASTERN ETHIOPIA.

Abstract: inadequate personal hygiene. The objective of this study was to determine the prevalence, intensity and associated risk factors of human intestinal parasitic infections of school children of Nicolas Bam Primary School,Harari Region, Eastern Ethiopia. In this crossectional descriptive study direct wet mount method, formol ether concentration technique and Kato Katz technique were employed to examine 403 stool samples. In addition, questionnaire survey was carried out to obtain infor...

EVALUATION OF THE ANTIMICROBIAL ACTIVITIES AND SCREENING OF THE PHYTOCHEMICAL COMPOSITION OF CRUDE EXTRACTS FROM THE LEAF EXUDATES OF ENDEMIC Aloe SPECIES (A. pirottae Berger and A. mcloughli

Abstract: The over increasing antibiotic resistance of pathogens has driven a global initiative towards the search for new sources of antimicrobial substances mainly from medicinal plants. Based on the existing traditional claim, this study investigated the antimicrobial activities and phytochemical composition of crude extracts of Aloe pirottae and Aloe mcloughlinii. Antibacterial activities of the extracts was conducted on test organisms using the disc diffusion method. The minimum inhibit...

Analysis of Chemical Composition of Cactus Pear (Opuntia ficus indica L.) Cladode Extract

Abstract: The present study was aimed at analyzing the chemical composition of the crude extract of cactus pear (Opuntia ficus-indica L.) cladode and examine its suitability for fungal culture media. For this purpose cactus pear cladode samples were collected from Haramaya University campus Model School area. The chemical composition analysis was done according to standard procedures. The growth of five fungal species (R. stoloinfer ATCC 1052, P. digitatum ATCC 2560, A. niger ATCC 1232, T. a...

ANALYSIS OF CHEMICAL COMPOSITION OF CACTUS PEAR (OPUNTIA FICUS-INDICA L.) CLADODE EXTRACT AND ITS ROLES IN THE PREPARATION OF FUNGAL CULTURE MEDIA

Abstract: The present study was aimed at analyzing the chemical composition of the crude extract of cactus pear (Opuntia ficus-indica L.) cladode and examine its suitability for fungal culture media. For this purpose cactus pear cladode samples were collected from Haramaya University campus Model School area. The chemical composition analysis was done according to standard procedures. The growth of five fungal species (R. stoloinfer ATCC 1052, P. digitatum ATCC 2560, A. niger ATCC 1232, T. a...

ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY THEPEOPLE OF WADERA WOREDA, EAST GUJI ZONE, OROMIA REGION, ETHIOPIA

Abstract: The study of indigenous knowledge on utilization of native plants as source of Medicine is important to conserve and preserve indigenous knowledge for next generation. This study was conduct to document indigenous knowledge and medicinal plants used by the people of Wadera District. A total of 100 respondents (age≥20) were selected to collect information on medicinal plants selected Kebele. Of these 10 key informant were selected purposely based on recommendation by local elders ...

PREVALENCE OF INTESTINAL PROTOZOAN PARASITIC INFECTION AND ITS ASSOCIATED RISK FACTORS AMONG STUDENTS OF YEIDWUHA PRIMARY SCHOOL, AMHARA NATIONAL REGIONAL STATE, ETHIOPIA

Abstract: Intestinal parasitic infections are growing problems worldwide and have been described as constituting the greatest worldwide cause of illnesses and deaths. Entamoeba histolytica / dispar and Giardia lamblia are major causes of infection among protozoan parasites which infect most school children. However, there was no research conducted so far in this regard in the selected study area. Thus, the major objective of the present study was to assess the prevalence of parasitic intesti...

PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES OF OIL EXTRACTS FROM SEEDS AND LEAVES OF GARDEN CRESS (Lepidium sativum L.)

Abstract: Garden cress is a fast growing annual herb that is native to Ethiopia. Its seeds are rich source of proteins, dietary fiber, omega-3 fatty acids, iron, other essential nutrients and phytochemicals. The aim of the present study was to investigate the physicochemical properties and biological activities of the oils from garden cress seeds and leaves using solvent extraction methods. The oil extraction was done in Soxhelt apparatus using petroleum ether as a solvent. The physicochemic...

PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI FROM RED BEET (Beta vulgaris L. ) LEAF AND ROOT PEELS

Abstract: The present study was intended to investigate phytochemical analysis and antioxidant activities of endophytic fungi isolates from red beetroot (Beta vulgaris L. )Leaf and Root peels . The red beet leaf and root samples were as potential source of endophytic fungi. The surface of samples were disinfectant. Fungal culturing and identifications of genera were made according to fungal identification manual. The phytochemical screening of methanolic extracts of Endophytic fungal isolate...

ANTIOXIDANT AND ANTIMICROBIAL POTENTIALS OF ENDOPHYTIC FUNGI FROM BANANA (Musa spp) PEEL AND PULP

Abstract: Banana is an edible fruit and herbaceous flowering plant belonging to genus musa and family musaceae.Banana is a source of natural antioxidants and has good antimicrobial potential. Banana Peel is nutritional and rich in phytochemical compounds than its pulp. Characterization of the antioxidant and antimicrobial activities of endophytic fungi extracted from banana peel and pulp is the main purpose of this study.The banana fruit sample was collected from dingete locality Gemechis di...


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