Abstract: We investigated the effect of bee species identity and harvesting methods on the chemical composition and antiradical activity of 53 honey samples, produced by six stingless bee species in western Kenya (Kakamega forest). Our results illustrate that none of the assayed parameters significantly varied between the honey samples harvested by “punching holes” (n = 25) and “squeezing” (n = 28) methods. By contrast, species identity drove significant differences in the assayed pa...
Abstract: The African citrus triozid (ACT), Trioza erytreae (Del Guercio), is the primary vector of Candidatus Liberibacter africanus (CLaf), the causative agent of Africa citrus greening disease (ACGD). This study evaluates the diversity of ACT parasitoids and further characterizes endosymbionts associated with both T. er y treae and its parasitoids that could be used as biological control agents of T. er y treae and management of ACGD. Mitochondrial cytochrome oxidase I gene was used to re...
Abstract: Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide. It can produce a range of debilitating infections, have a propensity for developing antimicrobial resistance, and present with a variety of potent virulence factors. This study investigated the sequence types (ST), phenotypic antimicrobial susceptibility profiles, and resistance and virulence genes among clinical isolates from urinary tract and skin and soft tissue infections. Fifty-six P. aeruginosa clin...
Abstract: Desert locust outbreak in East Africa is threatening livelihoods, food security, environment, and economic development in the region. The current magnitude of the desert locust invasion in East Africa is unprecedented and has not been witnessed for more than 70 years. Identifying the potential breeding sites of the pest is essential to carry out cost-effective and timely preventive measures before it inflicts significant damage. We accessed 9,134 desert locust occurrence records an...
Abstract: This study analyzed the protein content of ten edible insect species (using the Dumas method), then focused on the amino acid (AA) profiles of the six major commercially relevant species using HPLC (high-pressure (or performance) liquid chromatography). The protein contents varied significantly from 46.1% to 52.9% (dry matter); the Orthoptera representative yielding both the highest protein content and the highest values in three essential amino acids (EAAs). Regarding Lepidoptera ...
Abstract: Background Stingless bee honey (SBH) is a natural remedy and therapeutic agent traditionally used by local communities across the (sub-)tropics. Forest SBH represents a prime non-timber forest product (NTFP) with a potential to revitalize indigenous foodways and to generate income in rural areas, yet it is also used in a variety of non-food contexts that are poorly documented in sub-Saharan Africa and that collectively represent a significant part of the local traditional ecologica...
Abstract: Wheat bread is among stable foods that are nutritionally imbalanced, thus enrichment is crucial. We evaluated the nutritional impact of high-valued wheat bread enriched with varying levels of meat powder from hen fed diet with insect (Hermetia illucens)-based meal. Crude protein and ash in bread increased with increasing inclusion of meat powder. Limiting amino acids like lysine and threonine in enriched bread products increased by 3.0–4.5 and 1.8–3.1-folds, respectively. Omega...
Abstract: Tungiasis, a neglected tropical parasitosis, disproportionately afects children. Few empirical studies have reported neurocognitive and mental health outcomes of children with ectoparasitic skin diseases like tungiasis. Pathophysiology of tungiasis suggests it could detrimentally afect cognition and behaviour. This study pioneered the investigation of neurocognitive and mental health outcomes in children with tungiasis
Abstract: Tomato, Solanum lycopersicum L. (Solanaceae) is severely damaged by the South American tomato moth, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Surveys among producers confirmed that the use of synthetic insecticides increased since the invasion of T. absoluta. The risks associated with the residual effect of insecticides in edible food and the negative effects it may have on ecosystem services,also increased. The aim of this project was to investigate the management of T. ...
Abstract: Malaria continues to be among the top leading causes of illness and deaths in Africa. Its elimination is challenging in high transmission areas using insecticide-based intervention tools alone. Understanding the ecology and behaviour of disease vectors, including the olfactory cues, are essential for the development of novel, insecticide-free control tools. Recent work has highlighted that selected graminoid plants release volatile chemicals that attract malaria vectors. However, t...
Description: A thesis submitted to the Faculty of Science in fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Zoology, Kenyatta University.
Abstract: Anopheles mosquitoes are colonized by diverse microorganisms that may influence hosts‟ biology and vectorial capacity. Resident Anopheles bacterial symbionts isolated from these hosts are known to influence traits such as generational fitness, survival, host seeking,and infectivity of pathogens such asPlasmodium falciparum.Eukaryotic symbionts such as fungi residing in the gut of Anopheles gambiaecould confer similar phenotypes, but are undexplored. It was hypothesized that fungi...
Abstract: Dengue (DENV) and Yellow fever (YFV) viruses are medically important flaviviruses that are transmitted by Aedes mosquito vectors of the subgenus Stegomyia especially Aedes aegypti and Aedes bromeliae, respectively, in East Africa.Urbanization has been identified as a major driver in the emergence of these diseases because of the permissive environment it creates promoting the convergence of susceptible human hosts and local vectors. However, while dengue outbreaks have mainly been ...
Abstract: The African wild silkmoth, Gonometa postica Walker produces silk of high quality. A study on the spatial distribution and biology of G. postica on host and non-host plants and the parasitism rates and reproductive strategy of its parasitoids was conducted during the long and short rainy seasons in 2006 and 2007. Three sites, each in the Imba and Mumoni forests of Mwingi eastern Kenya, were selected for sampling. One hundred trees of the host plants of G. postica were sampled at eac...
Abstract: The livestock tropical bont tick, Amblyomma variegatum Fabricius, is a pest of major economic importance in Africa where it is the vector of Cowdria ruminantium that causes Heartwater, disease in cattle. Its control has relied mainly on commercial acaricides, which have many problems associated with, environmental pollution, development of resistant strains of ticks against acaricides and escalating costs. It has, therefore, become necessary to look for alternative methods of tick ...