Natural & Applied Sciences

Research Papers/Topics Natural & Applied Sciences

ISOLATION AND IDENTIFICATION OF POTENTIAL INDIGENOUS BACTERIA USEFUL FOR BIOREMEDIATION OF BREWERY EFFLUENTS

Abstract: The brewery industry consumes daily a large volume of water and chemicals and generates a high amount of nutrient-rich wastewater. The discharge of this wastewater into the environment without proper treatment causes serious and prolonged ill consequences on the ecosystem. Thus, the objectives of this study were to assess the physicochemical characteristics of Harar Brewery effluent; to isolate, identify and determine the efficiency of the indigenous bacteria in removing or reducin...

DIVERSITY AND ABUNDANCE OF AVIFAUNA: IMPLICATIONS FOR CONSERVATION AND ECOTOURISM DEVELOPMENT IN SUBA FOREST, CENTRAL ETHIOPIA

Abstract: Birds are among essential taxa in various ecosystems where their presence/absence and status indicate conditions of the habitats. Hence, a detailed understanding of species biogeography, diversity, and abundance is critical ecological information to evaluate or develop conservation strategies. The present study was conducted during dry and wet seasons from March 2021 to April 2021 and June 2021 to July 2021, respectively, to assess species diversity and abundance of avifauna as pot...

ISOLATION AND ANTIMICROBIAL SUSCEPTIBILITY TEST OF NON TYPHOIDAL SALMONELLA FROM RAW COW MILK AND ASSESSMENT OF HYGIENIC PRACTICES IN GURSUM DISTRICT, EASTERN HARARGE, ETHIOPIA

Abstract: Non-Typhoidal Salmonella represents an important human and animal pathogen world-wide. Most human non-typhoidal Salmonella (NTS) cases are foodborne, but each year infections are also acquired through direct or indirect animal contact. Epidemiological information on the prevalence of Salmonella is a prerequisite to develop appropriate control strategies. A cross sectional study was conducted from July 2022 to January 2023 to isolate and perform Antimicrobial susceptibility test of ...

CLIMATE VARIABILITY AND DETERMINANTS OF HOUSEHOLD FOOD SECURITY: THE CASE OF MIRAB BADAWACHO WOREDA, HADIYA ZONE SNNPR, ETHIOPIA

Abstract: Ethiopia is one of the most vulnerable countries of the world to the impacts of climate change and variability. However, this study took Mirab Badawacho woreda as a case and analyzed changes in local climate; status of household food security; and household level determinants of food security. Qualitative and quantitative methodologies have been employed. Socio-economic data were collected from three rural Kebeles of Mirab Badawacho Woreda. A total of 281 sample households were sel...

EFFECTS OF DAIRY PROCESSING AND HYGIENIC PRACTICES ON MICROBIAL QUALITY OFMILK AND YOGHURT IN HARAMAYA WOREDA,OROMIA REGIONAL STATE, ETHIOPIA

Abstract: Milk is an intricate or a complex biological fluid and by its nature, a good grawthmedium for many microorganisms.Becouse of its physico-chemical properties, its needs strict hygienic condition to avoid contamination of milk with microorganisms.Therefore, the microbial content of milk is a major feature in determining its quality.Microbial contamination can generally occure from three sources such as, with in the udder, exterior of the udder and surface of milk handling and storage...

ETHNOBOTANICAL STUDY OF TRADITIONAL MEDICINAL PLANTS USED IN SHIRKA WOREDA, EAST ARSI ZONE, OROMIA, ETHIOPIA

Abstract: An ethnobotanical study of traditional medicinal plants was conducted in Shirka woreda to document the indigenous plant-based traditional medical knowledge of the people of Shirka, Eastern Arsi zone, Oromia Region, Ethiopia. The main objective of this study was to identify traditional medicinal plant species, recognize human and livestock ailments that can be treated by traditional medicinal plants. A total of 384 informants were selected to collect ethno botanical information from...

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC FUNGI FROM COFFEE (Coffea arabicaL.) HUSK

Abstract: This study was intended to investigate the antioxidant and antimicrobial activities of endophytic fungi isolates from coffee husks. The coffee husk sample was collected from the local processor in Nensebo district, Arsi Zone, Ethiopia. The samples were surface sterilized before used as endophytic fungal culture. The nutrient value was prepared as per manufacturer instructions. Fungal culturing and identifications of genera were made according to fungal identification manual. Then, ...

ASSESSMENT OF MICROBIAL ANTAGONISM IN THE RHIZOSPHEREAND NON-RHIZOSPHERE OF POTATO (Solanum tuberosum L.) AT GADAB HASSASA DISTRICT, WEST ARSI ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: Potato is one of the most staple food sources for many people worldwide. The inoculation of antagonism with potatoes plays a very important role in the worled due to its contribution for human consumption. The rhizosphere is an important source of microbial antagonistic against soilborne fungal pathogens Rhizoctonia solani.This study focuse on the assessment of microbial antagonism in rhizosphere and non-rhizosphere of potato through investigation of potential microbial antagonism ...

ISOLATION OF Aspergillus oryzae AND SINGLE CELL PROTEIN PRODUCTION BY GROWN ON COFFEE CHERRY HUSK AND SAWDUST

Abstract: The rising global population pressure generates challenges to fulfill food requirements for the coming generations in adequate amounts. This suggests that the human population cannot continue to be entirely dependent on crops, animal husbandry and fisheries for food. Instead, efforts should be made to enhance the production of other potential alternative sources of food. To this end the current study has been undertaken to evaluate the production of single cell protein (SCP) from A...

PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICOBIAL ACTIVITIES OF (Plectranthus amboinicus (Lour)) OF LEAF AND STEM EXTRACTS

Abstract: Plectranthus amboinicus is knowns us traditional plant medicine in dif erent rural and urban community. However, production and usage of this traditional medicines require a scientific techniques and supports for the appropriate development of antibacterial drugs. The current study was intended to investigate phytochemical screening and antibacterial activities of Plectranthus amboinicus crude extracts, against pathogenic bacteria using the disc dif usion method. The minimum inhibi...

Production of Amylase from Thermophilic Bacteria from Harmukale Natural Hot Spring in Somali Region, Ethiopia

Abstract: Enzymes are biological catalysts, which initiate and speed up thousands of biochemical reactions in living cells. Among industrially important enzymes, amylases, protease and cellulases are getting more attention and importance because these enzymes have wide applications in many fields. For instance, amylases are used in various industries such as food, detergent, paper, textile, beverage, pharmaceutical and fine-chemical industries. Thermophiles are a potent source of thermos enz...

BIOSYNTHESIS OF CITRIC ACID FROM AVOCADO (Persea americana) FRUIT PEELS USING Aspergillus niger

Abstract: Due to its extensive use in the food and pharmaceutical industries, citric acid is an essential organic acid that is in high demand around the world. To meet this increasing demand, an effort has been made to use inexpensive agro-industrial waste products as carbohydrate sources for the production of citric acid using Aspergillus niger. Therefore, the present study was performed to produce citric acid from avocado (Persea americana) peels as a novel substrate through solid state fe...

PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICOBIAL ACTIVITIES OF (Plectranthus amboinicus (Lour)) OF LEAF AND STEM EXTR

Abstract: Plectranthus amboinicus is knowns us traditional plant medicine in dif erent rural and urban community. However, production and usage of this traditional medicines require a scientific techniques and supports for the appropriate development of antibacterial drugs. The current study was intended to investigate phytochemical screening and antibacterial activities of Plectranthus amboinicus crude extracts, against pathogenic bacteria using the disc dif usion method. The minimum inhibi...

Ethnobotanical Study of Traditional Medicinal Plants in DigelunaTijo District, Arsi Zone Oromia Regional State, Ethiopia

Abstract: Ethiopia has rich flora with different plant species having use in health care system based on local indigenous knowledge. However, documented ethnobotanical information was lacking in DigelunaTijo district. Hence, the aim of this study was to identify and document the use and conservation status of medicinal plants in Digeluna Tijo district. Ethnobotanical information on plants of local medicine was gathered through group discussion, semi structure interviews involving 129 informa...

ENZYME CHARACTERIZATION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF PROTEASE FROM CHICKPEA(Cicerarietinum L.) SEED AND LEAF EXTRACTS

Abstract: Chickpea proteins are appreciated due to their high biological values, well balanced amino acid content and low content of anti-nutritional factors. The present study was undertaken to investigate enzyme Characterization, antioxidant and antimicrobial activities of protease extract from chickpea (Cicerarietinum L.). The chickpea seed and leaf samples collected from farmer’s field were used for protease enzyme extraction. The protein concentration was determined by Lowry method. P...


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