This study uses the model organism, C. elegans, to investigate its sensitivity and response to static magnetic fields. Wild-type C. elegans are put into microfluidic channels and exposed to permanent magnets for five cycles of thirty-second time intervals at field strengths ranging from 5 milli Tesla to 120 milli Tesla. Recorded and analyzed with custom software, the results of the worm's movement - the average velocity, turning and curling percentage - were compared to control experiments...
We present the NERV, a nonmechanical, unidirectional valve, to control the locomotion of Caenorhabditis elegant (C. elegans) in microfluidic devices. This valve is created by establishing a region of lateral electric field which can be toggled between on and off states. We observed that C. elegans do not prefer to advance into this region when the field lines are facing their movement, so when they reach the boundary of the NERV, they partway enter the field, retreat, and switch direction. We...
A nonmechanical, unidirectional valve, the Nematode Electrotactic-Response Valve (NERV), has been developed to control the motion of Caenorhabditis elegant (C. elegans) in microfluidic devices. By forming a region of lateral electric field, which can be switched or reversed, the NERV prevents worms from advancing through it if the field lines are angled against the movement of the worms. Upon arriving at the boundary of the NERV, the worms penetrate the field area briefly and then retreat and...
This research paper presents a microfluidic platform for analyzing the effects of drugs and their dosage on the locomotion behavior of both free-living and parasitic nematodes. The chip provides a higher level of sensitivity for drug screening and can monitor four different nematode parameters in real time. It is a cross-species and cross-drug platform that is far more sophisticated than existing nematode motility and migration assays. The device consists of two parts: behavioral microchannel...
Filipinos infected with HIV-AIDS continued to balloon, notwithstanding the intervention through comprehensive prevention, dissemination, and control programs. In Eastern Visayas, 69 cases were coming from the most risk population, primarily Men Having Sex with Men (MSM), either homosexual, heterosexual, or bisexual modes of transmission. Bearing this epidemic, sexual networking, using the internet for sex served as cruising sites for casual sex, and promiscuity may be accounted for this surge...
What is an anxiety disorder? Anxiety disorders are a group of conditions marked by extreme or pathological anxiety or fear. In Australia anxiety disorders are the most common of the psychiatric disorders, with one in four people experiencing an anxiety disorder at some time in their life. Anxiety disorders have the potential to interfere with a persons work, family and social life. They tend to be persistent and can be disabling. Anxiety is a normal response to a threatening situation and can...
Normal anatomy and physiology Water comprises 60% of the body weight of an average adult, the total body water is divided functionally into the extracellular (ECF = 20% of body weight) and the intracellular fluid spaces (ICF = 40% of body weight) separated by the cell membrane. The ECF is further divided into the intravascular (within the circulation) and the interstitial (extravascular fluid surrounding the cells) fluid space.
This course focuses on building on knowledge of psychosocial development from childhood to adulthood and the understanding of human behavior in health and illness and the knowledge acquired from NSC 314 (Mental Health Nursing and Psychiatric Nursing I) and NSC 412 (mental health and psychiatric nursing II) It is designed to equip the students to completely employ nursing process and evidence base nursing practice in the development of nursing care of Psychiatric clients. The course will expos...
Abstract Both research and clinical decision making rely on measurement scales. These scales vary with regard to their psychometric properties, ease of administration, dimensions covered by the scale, and other properties. This article reviews the main psychometric characteristics of scales and assesses their utility. © 2010 Elsevier Inc. All rights reserved.
INTRODUCTION There is no Health without “MENTAL HEALTH” but many people seldom talk and some only pay little attention to doing things to promote their mental health. Many people who have mental health challenges may not even know that they have such not to talk of seeking help! Hello, welcome to this course. We are happy to have you doing NSC 314 – Mental Health and Psychiatric Nursing I. You would have done some lectures in Mental Health and Psychiatric Nursing when you were in the ba...
This paper looks at a movement-based assay to analyze adaptability in Caenorhabditis elegans locomotion behavior. A series of sinusoidal microchannels of fixed wavelength and varying amplitude, with the width being similar to the body diameter of the organism, are used for this purpose. The worms are allowed to enter the channel from the input and transition towards the output port. In sections that are similar to the worms' undulations, the movement is fast and steady. However, as the channe...
This paper looks at a movement-based assay to analyze adaptability in Caenorhabditis elegans locomotion behavior. A series of sinusoidal microchannels of fixed wavelength and varying amplitude, with the width being similar to the body diameter of the organism, are used for this purpose. The worms are allowed to enter the channel from the input and transition towards the output port. In sections that are similar to the worms' undulations, the movement is fast and steady. However, as the channe...
This study uses the model organism, C. elegans, to investigate its sensitivity and response to static magnetic fields. Wild-type C. elegans are put into microfluidic channels and exposed to permanent magnets for five cycles of thirty-second time intervals at field strengths ranging from 5 milli Tesla to 120 milli Tesla. Recorded and analyzed with custom software, the results of the worm's movement - the average velocity, turning and curling percentage - were compared to control experiments...
To evaluate the level of infestation of the soybean cyst nematode (SCN), Heterodera glycines, in the field, egg population densities are determined from soil samples. Sucrose centrifugation is a common technique for separating debris from the extracted SCN eggs. We have developed a procedure, however, that employs OptiPrep as a density gradient medium, with improved extraction and recovery of the eggs compared to the sucrose centrifugation technique. Also, we have built computerized methods t...
The transmembrane proteins known as ion channels play a role in controlling and preserving the ionic concentrations across the cell membrane. Modeling the flux of ions in and out of these channels on an atomic level is essential for understanding several neurological diseases and related pharmaceutical discoveries. Recent experimental research has provided information on the channel's physical structure which can be used to create realistic ion transport models. Different trajectories exist f...